GTF2IRD1 Antibody - #DF14018

Product: GTF2IRD1 Antibody
Catalog: DF14018
Description: Rabbit polyclonal antibody to GTF2IRD1
Application: ELISA(peptide)
Reactivity: Human
Mol.Wt.: 106kD; 106kD(Calculated).
Uniprot: Q9UHL9

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
GTF2IRD1 Antibody detects endogenous levels of total GTF2IRD1.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

A synthesized peptide derived from GTF2IRD1.

Uniprot:

Q9UHL9(GT2D1_HUMAN) >>Visit HPA database.

Gene(ID):
GTF2IRD1(9569)
Expression:
Q9UHL9 GT2D1_HUMAN:

Highly expressed in adult skeletal muscle, heart, fibroblast, bone and fetal tissues. Expressed at lower levels in all other tissues tested.

Sequence:
MALLGKRCDVPTNGCGPDRWNSAFTRKDEIITSLVSALDSMCSALSKLNAEVACVAVHDESAFVVGTEKGRMFLNARKELQSDFLRFCRGPPWKDPEAEHPKKVQRGEGGGRSLPRSSLEHGSDVYLLRKMVEEVFDVLYSEALGRASVVPLPYERLLREPGLLAVQGLPEGLAFRRPAEYDPKALMAILEHSHRIRFKLKRPLEDGGRDSKALVELNGVSLIPKGSRDCGLHGQAPKVPPQDLPPTATSSSMASFLYSTALPNHAIRELKQEAPSCPLAPSDLGLSRPMPEPKATGAQDFSDCCGQKPTGPGGPLIQNVHASKRILFSIVHDKSEKWDAFIKETEDINTLRECVQILFNSRYAEALGLDHMVPVPYRKIACDPEAVEIVGIPDKIPFKRPCTYGVPKLKRILEERHSIHFIIKRMFDERIFTGNKFTKDTTKLEPASPPEDTSAEVSRATVLDLAGNARSDKGSMSEDCGPGTSGELGGLRPIKIEPEDLDIIQVTVPDPSPTSEEMTDSMPGHLPSEDSGYGMEMLTDKGLSEDARPEERPVEDSHGDVIRPLRKQVELLFNTRYAKAIGISEPVKVPYSKFLMHPEELFVVGLPEGISLRRPNCFGIAKLRKILEASNSIQFVIKRPELLTEGVKEPIMDSQGTASSLGFSPPALPPERDSGDPLVDESLKRQGFQENYDARLSRIDIANTLREQVQDLFNKKYGEALGIKYPVQVPYKRIKSNPGSVIIEGLPPGIPFRKPCTFGSQNLERILAVADKIKFTVTRPFQGLIPKPDEDDANRLGEKVILREQVKELFNEKYGEALGLNRPVLVPYKLIRDSPDAVEVTGLPDDIPFRNPNTYDIHRLEKILKAREHVRMVIINQLQPFAEICNDAKVPAKDSSIPKRKRKRVSEGNSVSSSSSSSSSSSSNPDSVASANQISLVQWPMYMVDYAGLNVQLPGPLNY

PTMs - Q9UHL9 As Substrate

Site PTM Type Enzyme
K94 Ubiquitination
S113 Phosphorylation
K130 Ubiquitination
S193 Phosphorylation
K201 Ubiquitination
K212 Sumoylation
K225 Sumoylation
K271 Sumoylation
K271 Ubiquitination
K308 Sumoylation
K308 Ubiquitination
S329 Phosphorylation
K337 Sumoylation
K337 Ubiquitination
K379 Sumoylation
K379 Ubiquitination
K395 Ubiquitination
T403 Phosphorylation
Y404 Phosphorylation
S418 Phosphorylation
K436 Sumoylation
K436 Ubiquitination
K443 Sumoylation
K443 Ubiquitination
S448 Phosphorylation
T461 Phosphorylation
S471 Phosphorylation
S475 Phosphorylation
S477 Phosphorylation
K495 Sumoylation
K567 Sumoylation
Y577 Phosphorylation
K579 Sumoylation
K579 Ubiquitination
S584 Phosphorylation
K638 Sumoylation
S654 Phosphorylation
K684 Sumoylation
K684 Ubiquitination
T704 Phosphorylation
K715 Ubiquitination
K716 Ubiquitination
Y717 Phosphorylation
K724 Sumoylation
K724 Ubiquitination
K754 Ubiquitination
K787 Sumoylation
K799 Ubiquitination
K807 Ubiquitination
Y814 Phosphorylation
Y828 Phosphorylation
K829 Sumoylation
K829 Ubiquitination
S834 Phosphorylation
S906 Phosphorylation
S914 Phosphorylation
S915 Phosphorylation
S916 Phosphorylation
S917 Phosphorylation

Research Backgrounds

Function:

May be a transcription regulator involved in cell-cycle progression and skeletal muscle differentiation. May repress GTF2I transcriptional functions, by preventing its nuclear residency, or by inhibiting its transcriptional activation. May contribute to slow-twitch fiber type specificity during myogenesis and in regenerating muscles. Binds troponin I slow-muscle fiber enhancer (USE B1). Binds specifically and with high affinity to the EFG sequences derived from the early enhancer of HOXC8 (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in adult skeletal muscle, heart, fibroblast, bone and fetal tissues. Expressed at lower levels in all other tissues tested.

Subunit Structure:

Interacts with the retinoblastoma protein (RB1) via its C-terminus.

Family&Domains:

The N-terminal half may have an activating activity.

Belongs to the TFII-I family.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Genetic Information Processing > Transcription > Basal transcription factors.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

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