PDE6 delta Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Rat lung, using PDE6 delta Antibody.

Product:	PDE6 delta Antibody
Catalog:	DF13962
Description:	Rabbit polyclonal antibody to PDE6 delta
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	17kD; 17kD(Calculated).
Uniprot:	O43924

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

PDE6 delta Antibody detects endogenous levels of total PDE6 delta.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

5''-cyclic phosphodiesterase subunit delta; GMP PDE delta; GMP-PDE delta; p17 protein; PDE 6; PDE 6D; PDE D; PDE6; PDE6D; PDE6D_HUMAN; PDED; Phosphodiesterase 6D cGMP specific; Phosphodiesterase 6D cGMP specific rod delta; Protein p17; Retinal rod photoreceptor cGMP phosphodiesterase delta; Retinal rod rhodopsin sensitive cGMP 3' 5' cyclic phosphodiesterase subunit delta; Retinal rod rhodopsin-sensitive cGMP 3'';

Immunogens

Immunogen:

A synthesized peptide derived from Human PDE6 delta.

Uniprot:

O43924(PDE6D_HUMAN) >>Visit HPA database.

Gene(ID):

PDE6D(5147)

Expression:

O43924 PDE6D_HUMAN:

Widely expressed. Detected in various tissues including spleen, prostate gland, testis, ovary, small intestine, colon, retina, and peripheral blood.

Sequence:

O43924 PDE6D_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSAKDERAREILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVSRELNFSSTEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPASVLTGNVIIETKFFDDDLLVSTSRVRLFYV

Research Backgrounds

O43924_PDE6D_HUMAN

Function:

Promotes the release of prenylated target proteins from cellular membranes. Modulates the activity of prenylated or palmitoylated Ras family members by regulating their subcellular location. Required for normal ciliary targeting of farnesylated target proteins, such as INPP5E. Modulates the subcellular location of target proteins by acting as a GTP specific dissociation inhibitor (GDI) (By similarity). Increases the affinity of ARL3 for GTP by several orders of magnitude. Stabilizes ARL3-GTP by decreasing the nucleotide dissociation rate (By similarity).

Subcellular Location:

Cytoplasm>Cytosol. Cytoplasmic vesicle membrane>Peripheral membrane protein. Cytoplasm>Cytoskeleton>Cilium basal body.

Tissue Specificity:

Widely expressed. Detected in various tissues including spleen, prostate gland, testis, ovary, small intestine, colon, retina, and peripheral blood.

Subunit Structure:

Interacts with the prenylated catalytic subunits of PDE6, an oligomer composed of two catalytic chains (PDE6A and PDE6B) and two inhibitory chains (gamma); has no effect on enzyme activity but promotes the release of the prenylated enzyme from cell membrane (By similarity). Interacts with prenylated GRK1 and GRK7 (By similarity). Interacts with prenylated Ras family members, including RAP2A and RAP2C (By similarity). Interacts with prenylated RHEB and NRAS. Interacts with prenylated HRAS and KRAS. Interacts with RAB13 (prenylated form); dissociates RAB13 from membranes. Interacts with prenylated INPP5E. Interacts with RPGR. Interacts with ARL2. Interacts with ARL3; the interaction occurs specifically with the GTP-bound form of ARL3. Interaction with ARL2 and ARL3 promotes release of farnesylated cargo proteins.

Family&Domains:

Belongs to the PDE6D/unc-119 family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

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PDE6 delta Antibody - #DF13962