CRB3 Antibody - #DF13932

(2)  
Product: CRB3 Antibody
Catalog: DF13932
Description: Rabbit polyclonal antibody to CRB3
Application: IHC IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 13~30kD; 13kD(Calculated).
Uniprot: Q9BUF7

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
CRB3 Antibody detects endogenous levels of total CRB3.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CRB3; CRUM3_HUMAN; crumbs homolog 3 (Drosophila); Crumbs protein homolog 3; crumbs, Drosophila, homolog of, 3; PRO1158; protein crumbs homolog 3; UNQ588;

Immunogens

Immunogen:

A synthesized peptide derived from Human CRB3.

Uniprot:

Q9BUF7(CRUM3_HUMAN) >>Visit HPA database.

Gene(ID):
CRB3(92359)
Expression:
Q9BUF7 CRUM3_HUMAN:

Preferentially expressed in epithelial tissues. Expressed at high levels in lung, kidney, retina, colon and mammary glands. Expressed at moderate levels in liver, spleen, pancreas, placenta and prostate.

Sequence:
MANPGLGLLLALGLPFLLARWGRAWGQIQTTSANENSTVLPSSTSSSSDGNLRPEAITAIIVVFSLLAALLLAVGLALLVRKLREKRQTEGTYRPSSEEQVGARVPPTPNLKLPPEERLI

Research Backgrounds

Function:

Involved in the establishment of cell polarity in mammalian epithelial cells. Regulates the morphogenesis of tight junctions.

Subcellular Location:

Apical cell membrane>Single-pass type I membrane protein. Cell junction>Tight junction.
Note: Localizes primarily to the apical membrane with a small fraction in the upper part of tight junctions of epithelial cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Preferentially expressed in epithelial tissues. Expressed at high levels in lung, kidney, retina, colon and mammary glands. Expressed at moderate levels in liver, spleen, pancreas, placenta and prostate.

Subunit Structure:

Interacts with MPP5, PARD6A and PATJ.

Family&Domains:

The PDZ-binding motif is involved in the interactions with PARD6A and MPP5.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

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