PTP1B Antibody - #AF6205

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Product: PTP1B Antibody
Catalog: AF6205
Description: Rabbit polyclonal antibody to PTP1B
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 49kDa; 50kD(Calculated).
Uniprot: P18031
RRID: AB_2835086

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PTP1B Antibody detects endogenous levels of total PTP1B.
RRID:
AB_2835086
Cite Format: Affinity Biosciences Cat# AF6205, RRID:AB_2835086.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

PTP1B; Non receptor tyrosine phosphatase 1; Protein phosphotyrosylphosphatase 1B; Protein tyrosine phosphatase 1B; Protein tyrosine phosphatase non receptor type 1; Protein tyrosine phosphatase placental; Protein-tyrosine phosphatase 1B; PTN1_HUMAN; PTP 1B; PTP-1B; PTPN 1; PTPN1; Tyrosine protein phosphatase non receptor type 1; Tyrosine-protein phosphatase non-receptor type 1;

Immunogens

Immunogen:
Uniprot:

P18031(PTN1_HUMAN) >>Visit HPA database.

Gene(ID):
PTPN1(5770)
Expression:
P18031 PTN1_HUMAN:

Expressed in keratinocytes (at protein level).

Description:
The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues.
Sequence:
MEMEKEFEQIDKSGSWAAIYQDIRHEASDFPCRVAKLPKNKNRNRYRDVSPFDHSRIKLHQEDNDYINASLIKMEEAQRSYILTQGPLPNTCGHFWEMVWEQKSRGVVMLNRVMEKGSLKCAQYWPQKEEKEMIFEDTNLKLTLISEDIKSYYTVRQLELENLTTQETREILHFHYTTWPDFGVPESPASFLNFLFKVRESGSLSPEHGPVVVHCSAGIGRSGTFCLADTCLLLMDKRKDPSSVDIKKVLLEMRKFRMGLIQTADQLRFSYLAVIEGAKFIMGDSSVQDQWKELSHEDLEPPPEHIPPPPRPPKRILEPHNGKCREFFPNHQWVKEETQEDKDCPIKEEKGSPLNAAPYGIESMSQDTEVRSRVVGGSLRGAQAASPAKGEPSLPEKDEDHALSYWKPFLVNMCVATVLTAGAYLCYRFLFNSNT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P18031 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K5 Ubiquitination
K12 Ubiquitination
S13 Phosphorylation
S15 Phosphorylation
Y20 Phosphorylation
C32 S-Nitrosylation
Y46 Phosphorylation
S50 Phosphorylation P49759 (CLK1) , P31749 (AKT1) , P49760 (CLK2)
S55 Phosphorylation
K58 Ubiquitination
Y66 Phosphorylation P00533 (EGFR) , P06213 (INSR)
S70 Phosphorylation
K73 Ubiquitination
C92 S-Nitrosylation
K116 Ubiquitination
S118 Phosphorylation
K120 Ubiquitination
K128 Ubiquitination
K131 Acetylation
K131 Ubiquitination
K141 Acetylation
S146 Phosphorylation
K150 Ubiquitination
Y152 Phosphorylation P06213 (INSR)
Y153 Phosphorylation P06213 (INSR)
S201 Phosphorylation
S205 Phosphorylation
C215 S-Nitrosylation
S222 Phosphorylation
T224 Phosphorylation
K239 Ubiquitination
S242 Phosphorylation P49759 (CLK1) , P49760 (CLK2)
S243 Phosphorylation P49759 (CLK1) , P49760 (CLK2)
K247 Ubiquitination
S286 Phosphorylation P53350 (PLK1)
S295 Phosphorylation
K314 Ubiquitination
K323 Ubiquitination
K335 Sumoylation
K335 Ubiquitination
T338 Phosphorylation
K347 Sumoylation
K350 Ubiquitination
S352 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
Y359 Phosphorylation
S363 Phosphorylation
S365 Phosphorylation
T368 Phosphorylation
S378 Phosphorylation P68400 (CSNK2A1) , P17252 (PRKCA) , P19784 (CSNK2A2)
R380 Methylation
S386 Phosphorylation P06493 (CDK1) , P68400 (CSNK2A1) , P19784 (CSNK2A2)
S393 Phosphorylation P53350 (PLK1)

Research Backgrounds

Function:

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.

PTMs:

Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.

Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50.

S-nitrosylation of Cys-215 inactivates the enzyme activity.

Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.

Subcellular Location:

Endoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Interacts with EPHA3 at the cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in keratinocytes (at protein level).

Subunit Structure:

Interacts with EPHA3 (phosphorylated); dephosphorylates EPHA3 and may regulate its trafficking and function. Interacts with MET.

Family&Domains:

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

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