EDD Antibody - #AF0268

(2)  
Product: EDD Antibody
Catalog: AF0268
Description: Rabbit polyclonal antibody to EDD
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Zebrafish, Horse, Sheep, Rabbit, Xenopus
Mol.Wt.: 309kDa; 309kD(Calculated).
Uniprot: O95071
RRID: AB_2833442

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Zebrafish(100%), Horse(100%), Sheep(88%), Rabbit(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
EDD Antibody detects endogenous levels of total EDD.
RRID:
AB_2833442
Cite Format: Affinity Biosciences Cat# AF0268, RRID:AB_2833442.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

4432411E13Rik; AW549941; C77315; D030042K14; DD5; E3 identified by differential display; E3 ubiquitin protein ligase, HECT domain containing, 1; E3 ubiquitin-protein ligase; E3 ubiquitin-protein ligase UBR5; EDD 1; EDD; EDD1; FLJ11310; HECT domain-containing 1; hHYD; HYD; Hyperplastic discs protein homolog; Hyperplastic discs, Drosophila, homolog of; KIAA0896; MGC57263; mKIAA0896; Progestin induced protein; Progestin-induced protein; Rat100; Ubiquitin protein ligase; Ubiquitin protein ligase E3 component n recognin 5; UBR5; UBR5_HUMAN;

Immunogens

Immunogen:
Uniprot:

O95071(UBR5_HUMAN) >>Visit HPA database.

Gene(ID):
UBR5(51366)
Expression:
O95071 UBR5_HUMAN:

Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.

Description:
UBR5 E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.
Sequence:
MTSIHFVVHPLPGTEDQLNDRLREVSEKLNKYNLNSHPPLNVLEQATIKQCVVGPNHAAFLLEDGRVCRIGFSVQPDRLELGKPDNNDGSKLNSNSGAGRTSRPGRTSDSPWFLSGSETLGRLAGNTLGSRWSSGVGGSGGGSSGRSSAGARDSRRQTRVIRTGRDRGSGLLGSQPQPVIPASVIPEELISQAQVVLQGKSRSVIIRELQRTNLDVNLAVNNLLSRDDEDGDDGDDTASESYLPGEDLMSLLDADIHSAHPSVIIDADAMFSEDISYFGYPSFRRSSLSRLGSSRVLLLPLERDSELLRERESVLRLRERRWLDGASFDNERGSTSKEGEPNLDKKNTPVQSPVSLGEDLQWWPDKDGTKFICIGALYSELLAVSSKGELYQWKWSESEPYRNAQNPSLHHPRATFLGLTNEKIVLLSANSIRATVATENNKVATWVDETLSSVASKLEHTAQTYSELQGERIVSLHCCALYTCAQLENSLYWWGVVPFSQRKKMLEKARAKNKKPKSSAGISSMPNITVGTQVCLRNNPLYHAGAVAFSISAGIPKVGVLMESVWNMNDSCRFQLRSPESLKNMEKASKTTEAKPESKQEPVKTEMGPPPSPASTCSDASSIASSASMPYKRRRSTPAPKEEEKVNEEQWSLREVVFVEDVKNVPVGKVLKVDGAYVAVKFPGTSSNTNCQNSSGPDADPSSLLQDCRLLRIDELQVVKTGGTPKVPDCFQRTPKKLCIPEKTEILAVNVDSKGVHAVLKTGNWVRYCIFDLATGKAEQENNFPTSSIAFLGQNERNVAIFTAGQESPIILRDGNGTIYPMAKDCMGGIRDPDWLDLPPISSLGMGVHSLINLPANSTIKKKAAVIIMAVEKQTLMQHILRCDYEACRQYLMNLEQAVVLEQNLQMLQTFISHRCDGNRNILHACVSVCFPTSNKETKEEEEAERSERNTFAERLSAVEAIANAISVVSSNGPGNRAGSSSSRSLRLREMMRRSLRAAGLGRHEAGASSSDHQDPVSPPIAPPSWVPDPPAMDPDGDIDFILAPAVGSLTTAATGTGQGPSTSTIPGPSTEPSVVESKDRKANAHFILKLLCDSVVLQPYLRELLSAKDARGMTPFMSAVSGRAYPAAITILETAQKIAKAEISSSEKEEDVFMGMVCPSGTNPDDSPLYVLCCNDTCSFTWTGAEHINQDIFECRTCGLLESLCCCTECARVCHKGHDCKLKRTSPTAYCDCWEKCKCKTLIAGQKSARLDLLYRLLTATNLVTLPNSRGEHLLLFLVQTVARQTVEHCQYRPPRIREDRNRKTASPEDSDMPDHDLEPPRFAQLALERVLQDWNALKSMIMFGSQENKDPLSASSRIGHLLPEEQVYLNQQSGTIRLDCFTHCLIVKCTADILLLDTLLGTLVKELQNKYTPGRREEAIAVTMRFLRSVARVFVILSVEMASSKKKNNFIPQPIGKCKRVFQALLPYAVEELCNVAESLIVPVRMGIARPTAPFTLASTSIDAMQGSEELFSVEPLPPRPSSDQSSSSSQSQSSYIIRNPQQRRISQSQPVRGRDEEQDDIVSADVEEVEVVEGVAGEEDHHDEQEEHGEENAEAEGQHDEHDEDGSDMELDLLAAAETESDSESNHSNQDNASGRRSVVTAATAGSEAGASSVPAFFSEDDSQSNDSSDSDSSSSQSDDIEQETFMLDEPLERTTNSSHANGAAQAPRSMQWAVRNTQHQRAASTAPSSTSTPAASSAGLIYIDPSNLRRSGTISTSAAAAAAALEASNASSYLTSASSLARAYSIVIRQISDLMGLIPKYNHLVYSQIPAAVKLTYQDAVNLQNYVEEKLIPTWNWMVSIMDSTEAQLRYGSALASAGDPGHPNHPLHASQNSARRERMTAREEASLRTLEGRRRATLLSARQGMMSARGDFLNYALSLMRSHNDEHSDVLPVLDVCSLKHVAYVFQALIYWIKAMNQQTTLDTPQLERKRTRELLELGIDNEDSEHENDDDTNQSATLNDKDDDSLPAETGQNHPFFRRSDSMTFLGCIPPNPFEVPLAEAIPLADQPHLLQPNARKEDLFGRPSQGLYSSSASSGKCLMEVTVDRNCLEVLPTKMSYAANLKNVMNMQNRQKKEGEEQPVLPEETESSKPGPSAHDLAAQLKSSLLAEIGLTESEGPPLTSFRPQCSFMGMVISHDMLLGRWRLSLELFGRVFMEDVGAEPGSILTELGGFEVKESKFRREMEKLRNQQSRDLSLEVDRDRDLLIQQTMRQLNNHFGRRCATTPMAVHRVKVTFKDEPGEGSGVARSFYTAIAQAFLSNEKLPNLECIQNANKGTHTSLMQRLRNRGERDREREREREMRRSSGLRAGSRRDRDRDFRRQLSIDTRPFRPASEGNPSDDPEPLPAHRQALGERLYPRVQAMQPAFASKITGMLLELSPAQLLLLLASEDSLRARVDEAMELIIAHGRENGADSILDLGLVDSSEKVQQENRKRHGSSRSVVDMDLDDTDDGDDNAPLFYQPGKRGFYTPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLNRHVIKVLLGRKVNWHDFAFFDPVMYESLRQLILASQSSDADAVFSAMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLVVAEQPLHAMRKGLLDVLPKNSLEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Xenopus
100
Zebrafish
100
Rabbit
100
Sheep
88
Pig
0
Bovine
0
Dog
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95071 As Substrate

Site PTM Type Enzyme
T2 Acetylation
K28 Ubiquitination
K31 Ubiquitination
Y32 Phosphorylation
S36 Phosphorylation
K49 Ubiquitination
S73 Phosphorylation
R78 Methylation
K83 Ubiquitination
S90 Phosphorylation
K91 Ubiquitination
S108 Phosphorylation
S110 Phosphorylation
T119 Phosphorylation
S130 Phosphorylation
S133 Phosphorylation
S134 Phosphorylation
S139 Phosphorylation
S143 Phosphorylation
R146 Methylation
S154 Phosphorylation
R167 Methylation
S169 Phosphorylation
S174 Phosphorylation Q13315 (ATM)
S191 Phosphorylation Q13315 (ATM)
K200 Ubiquitination
S203 Phosphorylation
T212 Phosphorylation
S287 Phosphorylation
S289 Phosphorylation
S293 Phosphorylation
S294 Phosphorylation
S313 Phosphorylation
S327 Phosphorylation
K337 Ubiquitination
K346 Ubiquitination
T348 Phosphorylation
S352 Phosphorylation
K366 Ubiquitination
K394 Ubiquitination
K423 Ubiquitination
K442 Ubiquitination
K457 Ubiquitination
S578 Phosphorylation
K583 Ubiquitination
S598 Phosphorylation
T605 Phosphorylation
S612 Phosphorylation
S615 Phosphorylation
S625 Phosphorylation
S636 Phosphorylation
T637 Phosphorylation
K663 Acetylation
K663 Ubiquitination
K669 Ubiquitination
K672 Ubiquitination
K681 Ubiquitination
K720 Ubiquitination
K726 Ubiquitination
T734 Phosphorylation
K743 Ubiquitination
K761 Ubiquitination
S808 Phosphorylation
S928 Phosphorylation
S967 Phosphorylation
S971 Phosphorylation
R977 Methylation
R984 Methylation
S985 Phosphorylation
S995 Phosphorylation
R997 Methylation
S1018 Phosphorylation
K1109 Ubiquitination
T1115 Phosphorylation
T1135 Phosphorylation
K1141 Ubiquitination
S1227 Phosphorylation
K1248 Ubiquitination
K1305 Ubiquitination
T1306 Phosphorylation
S1308 Phosphorylation
S1312 Phosphorylation
K1340 Ubiquitination
S1347 Phosphorylation
K1351 Ubiquitination
S1355 Phosphorylation
S1375 Phosphorylation
S1481 Phosphorylation
S1532 Phosphorylation Q13315 (ATM)
S1549 Phosphorylation
S1551 Phosphorylation
S1610 Phosphorylation
S1679 Phosphorylation Q13315 (ATM)
S1728 Phosphorylation
T1729 Phosphorylation
S1732 Phosphorylation
T1736 Phosphorylation
S1740 Phosphorylation
S1741 Phosphorylation
Y1746 Phosphorylation
T1760 Phosphorylation
S1761 Phosphorylation
S1780 Phosphorylation
S1782 Phosphorylation
S1789 Phosphorylation
S1875 Phosphorylation
S1878 Phosphorylation
T1902 Phosphorylation
S1905 Phosphorylation
R1914 Methylation
T1965 Phosphorylation
T1966 Phosphorylation
T1969 Phosphorylation
S1990 Phosphorylation
T1998 Phosphorylation
S2001 Phosphorylation
S2011 Phosphorylation
S2026 Phosphorylation
S2028 Phosphorylation
T2030 Phosphorylation
K2063 Ubiquitination
S2071 Phosphorylation
Y2075 Phosphorylation
S2076 Phosphorylation
K2101 Ubiquitination
S2103 Phosphorylation
K2136 Ubiquitination
S2140 Phosphorylation
R2190 Methylation
T2213 Phosphorylation
K2221 Ubiquitination
S2241 Phosphorylation
T2270 Phosphorylation
K2282 Ubiquitination
S2289 Phosphorylation
K2320 Ubiquitination
S2325 Phosphorylation
R2353 Methylation
S2369 Phosphorylation
S2379 Phosphorylation
S2434 Phosphorylation
S2437 Phosphorylation
S2460 Phosphorylation
S2469 Phosphorylation
S2470 Phosphorylation
K2472 Ubiquitination
S2483 Phosphorylation
S2484 Phosphorylation
S2486 Phosphorylation
T2495 Phosphorylation
K2510 Ubiquitination
S2586 Phosphorylation Q13315 (ATM)
K2656 Ubiquitination
S2779 Phosphorylation

Research Backgrounds

Function:

E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. Ubiquitinates acetylated PCK1. Also acts as a regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.

Subunit Structure:

Binds TOPBP1. Associates with CDK9 and TFIIS/TCEA1 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulate target gene transcription (e.g. gamma fibrinogen/FGG) by recruiting their promoters. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and DCAF1 proteins (EDVP complex). Interacts directly with DYRK2. Interacts with PIH1D1.

(Microbial infection) Interacts with human T-cell leukemia virus 1/HTLV-1 protein HBZ; this interaction modulates HBZ stability.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

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