ONECUT2 Antibody - #DF13779
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Protocols
Product Info
Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
ONECUT2 Antibody detects endogenous levels of total ONECUT2.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
HGNC:8139; MGC120377; MGC120378; OC 2; OC2; One cut domain family member 2; ONECUT 2; ONECUT 2 homeodomain transcription factor; ONECUT 2 protein; ONECUT2 homeodomain transcription factor; ONECUT2 protein; Transcription factor ONECUT 2; Transcription factor ONECUT2;
Immunogens
Immunogen:
A synthesized peptide derived from Human ONECUT2.
Uniprot:
Gene(ID):
Sequence:
- O95948 ONEC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKAAYTAYRCLTKDLEGCAMNPELTMESLGTLHGPAGGGSGGGGGGGGGGGGGGPGHEQELLASPSPHHAGRGAAGSLRGPPPPPTAHQELGTAAAAAAAASRSAMVTSMASILDGGDYRPELSIPLHHAMSMSCDSSPPGMGMSNTYTTLTPLQPLPPISTVSDKFHHPHPHHHPHHHHHHHHQRLSGNVSGSFTLMRDERGLPAMNNLYSPYKEMPGMSQSLSPLAATPLGNGLGGLHNAQQSLPNYGPPGHDKMLSPNFDAHHTAMLTRGEQHLSRGLGTPPAAMMSHLNGLHHPGHTQSHGPVLAPSRERPPSSSSGSQVATSGQLEEINTKEVAQRITAELKRYSIPQAIFAQRVLCRSQGTLSDLLRNPKPWSKLKSGRETFRRMWKWLQEPEFQRMSALRLAACKRKEQEPNKDRNNSQKKSRLVFTDLQRRTLFAIFKENKRPSKEMQITISQQLGLELTTVSNFFMNARRRSLEKWQDDLSTGGSSSTSSTCTKA
Research Backgrounds
Function:
Transcriptional activator. Activates the transcription of a number of liver genes such as HNF3B.
Subcellular Location:
Nucleus.
Family&Domains:
Belongs to the CUT homeobox family.
References
1). ONECUT2 which is targeted by hsa-miR-15a-5p enhances stemness maintenance of gastric cancer stem cells. Experimental Biology and Medicine, 2021
(PubMed: 34365839)
[IF=3.2]
Application: IHC Species: Human Sample: GCSCs
Figure 1.
ONECUT2 is an oncogene in gastric cancer. (a) LIMMA R package was used for analyzing differential gene expression of STAD samples and adjacent normal tissues in TCGA Database. ONECUT2 and DEGs whose absLogFC ranked top 10 were exhibited in a histogram. P value < 0.001. (b) qPCR analysis of hsa-miR-15a-5p expression between STAD samples and adjacent normal tissues. (c, d) Hsa-miR-15a-5p expression in 30 pairs of STAD samples and their corresponding non-tumorous tissues. (e, f) Immunohistochemistry detected the expression and distribution of ONECUT2 in the STAD samples and adjacent normal tissues and photos were taken. (g, h) GEPIA website analyzed the correlation between ONECUT2 expression and the survival of STAD patients. (A color version of this figure is available in the online journal.)
Application: WB Species: Human Sample: GCSCs
Figure 4.
Hsa-miR-15a-5p regulates the transcription and translation of ONECUT2 in GCSCs. (a) qPCR analysis of hsa-miR-15a-5p and ONECUT2 in human gastric adenocarcinoma cell lines HGC-27, AGS, SGC-7901, MKN45 and normal human gastric mucosal epithelial cell line GES-1. *P<0.05, ***P<0.001, ##P<0.01, ###P<0.001, ∼ versus GES-1. (b) qPCR analysis of hsa-miR-15a-5p and ONECUT2 in parental, CD133+/CD44+ and CD133-/CD44- MKN45. **P<0.01, ###P<0.001, ∼ versus parental MKN45. (c) Western blot detected ONECUT2 expression in parental, CD133+/CD44+ and CD133-/CD44- MKN45. (d) Western blot detected ONECUT2 expression in CD133+/CD44+ MKN45 transfected with hsa-miR-15a-5p mimics, and in CD133-/CD44- MKN45 transfected with hsa-miR-15a-5p inhibitors. (e) The sequence of hsa-miR-15a-5p and its target ONECUT2. (f) We constructed pGL3-ONECUT2-WT and pGL3-ONECUT2-MUT. Then, CD133+/CD44+ MKN45 were transfected with hsa-miR-15a-5p mimics. (g, h) qPCR and Western blot verified the efficiency of ONECUT2 overexpression (OE) vector. (A color version of this figure is available in the online journal.)
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