MYOCD Antibody | Affinity Biosciences

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FIGURE 3 MBNL1 increases Myocardin expression by binding to UGCU at the 3'-UTR of Myocardin mRNA.

Product:	MYOCD Antibody
Catalog:	AF0023
Description:	Rabbit polyclonal antibody to MYOCD
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	100 kDa; 102kD(Calculated).
Uniprot:	Q8IZQ8

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

MYOCD Antibody detects endogenous levels of total MYOCD.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

MYCD; MYCD_HUMAN; Myocardin; Myocd;

Immunogens

Immunogen:

A synthesized peptide derived from human MYOCD, corresponding to a region within the internal amino acids.

Uniprot:

Q8IZQ8(MYCD_HUMAN) >>Visit HPA database.

Gene(ID):

MYOCD(93649)

Expression:

Q8IZQ8 MYCD_HUMAN:

Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.

Description:

Transcriptional factor that uses the canonical single or multiple CArG boxes DNA sequence. Binds CArG boxes only in the presence of serum response factor (SRF). Acts as a cofactor of SRF and modulates SRF-target genes. Regulates the expression of a set of cardiac and smooth muscle-specific genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage.

Sequence:

Q8IZQ8 MYCD_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTLLGSEHSLLIRSKFRSVLQLRLQQRRTQEQLANQGIIPPLKRPAEFHEQRKHLDSDKAKNSLKRKARNRCNSADLVNMHILQASTAERSIPTAQMKLKRARLADDLNEKIALRPGPLELVEKNILPVDSAVKEAIKGNQVSFSKSTDAFAFEEDSSSDGLSPDQTRSEDPQNSAGSPPDAKASDTPSTGSLGTNQDLASGSENDRNDSASQPSHQSDAGKQGLGPPSTPIAVHAAVKSKSLGDSKNRHKKPKDPKPKVKKLKYHQYIPPDQKAEKSPPPMDSAYARLLQQQQLFLQLQILSQQQQQQQHRFSYLGMHQAQLKEPNEQMVRNPNSSSTPLSNTPLSPVKNSFSGQTGVSSFKPGPLPPNLDDLKVSELRQQLRIRGLPVSGTKTALMDRLRPFQDCSGNPVPNFGDITTVTFPVTPNTLPNYQSSSSTSALSNGFYHFGSTSSSPPISPASSDLSVAGSLPDTFNDASPSFGLHPSPVHVCTEESLMSSLNGGSVPSELDGLDSEKDKMLVEKQKVINELTWKLQQEQRQVEELRMQLQKQKRNNCSEKKPLPFLAASIKQEEAVSSCPFASQVPVKRQSSSSECHPPACEAAQLQPLGNAHCVESSDQTNVLSSTFLSPQCSPQHSPLGAVKSPQHISLPPSPNNPHFLPSSSGAQGEGHRVSSPISSQVCTAQMAGLHSSDKVGPKFSIPSPTFSKSSSAISEVTQPPSYEDAVKQQMTRSQQMDELLDVLIESGEMPADAREDHSCLQKVPKIPRSSRSPTAVLTKPSASFEQASSGSQIPFDPYATDSDEHLEVLLNSQSPLGKMSDVTLLKIGSEEPHFDGIMDGFSGKAAEDLFNAHEILPGPLSPMQTQFSPSSVDSNGLQLSFTESPWETMEWLDLTPPNSTPGFSALTTSSPSIFNIDFLDVTDLNLNSSMDLHLQQW

PTMs - Q8IZQ8 As Substrate

Q8IZQ8

Site	PTM Type	Enzyme	Source
S74	Phosphorylation		Uniprot
K138	Sumoylation		Uniprot
S178	Phosphorylation		Uniprot
S229	Phosphorylation		Uniprot
T230	Phosphorylation		Uniprot
S242	Phosphorylation		Uniprot
K264	Methylation		Uniprot
S451	Phosphorylation	P49841 (GSK3B)	Uniprot
S455	Phosphorylation	P49841 (GSK3B)	Uniprot
S459	Phosphorylation	P49841 (GSK3B)	Uniprot
S463	Phosphorylation	P49841 (GSK3B)	Uniprot
K571	Sumoylation		Uniprot
K588	Acetylation		Uniprot
S626	Phosphorylation	P49841 (GSK3B)	Uniprot
S630	Phosphorylation	P49841 (GSK3B)	Uniprot
S634	Phosphorylation	P49841 (GSK3B)	Uniprot
S638	Phosphorylation	P49841 (GSK3B)	Uniprot
S704	Phosphorylation		Uniprot
T732	Phosphorylation		Uniprot
S734	Phosphorylation		Uniprot
S815	Phosphorylation	P28482 (MAPK1)	Uniprot
S862	Phosphorylation	P28482 (MAPK1)	Uniprot
S869	Phosphorylation	P28482 (MAPK1)	Uniprot
T896	Phosphorylation	P28482 (MAPK1)	Uniprot

Research Backgrounds

Q8IZQ8_MYCD_HUMAN

Function:

Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis) (By similarity).

PTMs:

Phosphorylation regulates negatively the intrinsic myocardin transcriptional activity.

Subcellular Location:

Nucleus.

Tissue Specificity:

Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.

Subunit Structure:

Homodimer. Interacts with SRF, its association does not depend on specific DNA sequences for ternary complex formation (By similarity). Interacts with MLLT7/FOXO4. Interacts (via C-terminal) with EP300 (via the CREB-binding domain). Interacts with HDAC4 and HDAC5 (By similarity). Interacts with MEF2C (By similarity).

Family&Domains:

The C-terminal region contains a general transcription activation domain. The N-terminal region, comprising a basic and a Gln-rich domain, confers transcriptional potency and specificity by mediating association with the MADS box of SRF. The basic domain may be required for nuclear localization. The SAP domain is important for transactivation and ternary complex formation (By similarity).

References

1). MBNL1 regulates isoproterenol‐induced myocardial remodelling in vitro and in vivo. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (PubMed: 33295096) [IF=5.3]

Application: WB Species: mice Sample: cardiomyocytes

FIGURE 3 MBNL1 increases Myocardin expression by binding to UGCU at the 3'-UTR of Myocardin mRNA. A, The 3'-UTR region of Myocardin contains the binding site of MBNL1. B, The binding of MBNL1 to Myocardin mRNA was validated using RIP. (n = 3, *, P < .05). C-F, The changes in Myocardin and MBNL1 in cardiomyocytes with overexpressed or silenced MBNL1 were measured using realtime PCR and Western blotting. (n = 3, *, P < .05, **, P < .01). G and H, Myocardin mRNA residues after 0, 20, 40 and 60 min in cardiomyocytes treated with Actinomycin D (ACD, 5 μg/mL) and with overexpressed or silenced MBNL1were measured using realtime PCR. I, The 3'-UTR region of Myocardin mRNA was transcribed in vitro and labelled with biotin. The combination of MBNL1 protein and 3'-UTR of Myocardin was detected using RNA pulldown assay. J, The RNA probes corresponding to nine prediction sites (probe # 10-18) and their corresponding antisense probes (probe # 1-9) were synthesized and biotin-labelled. The combination site of MBNL1 protein in the 3'-UTR of Myocardin was confirmed using RNA pull-down assay

2). LncRNA-Mhrt regulates cardiac hypertrophy by modulating the miR-145a-5p/KLF4/myocardin axis. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (PubMed: 31982428) [IF=5.0]

Application: WB Species: mouse Sample: primary cardiomyocytes

Fig. 2. |Mhrt regulates myocardin expression through KLF4 in primary cardiomyocytes.(D, E and F) Following knock down of endogenous KLF4 in primary cardiomyocytes, the effect of Mhrt on myocardin mRNA and protein levels was examined (n = 3, *,P < .05, **, P < .01, #, P > .05).

3). Myocardin Reverses Hypoxia-Inducible Factor-1α Mediated Phenotypic Modulation of Corpus Cavernosum Smooth Muscle Cells in Hypoxia Induced by. World Journal of Mens Health (PubMed: 35274501) [IF=4.8]

4). KLHL38 facilitates STS‐induced apoptosis in HL‐1 cells via myocardin degradation. IUBMB LIFE (PubMed: 35112472) [IF=4.6]

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MYOCD Antibody - #AF0023