CacyBP Antibody | Affinity Biosciences

Product:	CacyBP Antibody
Catalog:	AF0017
Description:	Rabbit polyclonal antibody to CacyBP
Application:	WB
Reactivity:	Human
Mol.Wt.:	26kD(Calculated).
Uniprot:	Q9HB71

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

CacyBP Antibody detects endogenous levels of total CacyBP.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CacyBP;Calcyclin binding protein;Calcyclin-binding protein;CYBP_HUMAN;GIG 5;GIG5;Growth inhibiting gene 5;Growth inhibiting gene 5 protein;hCacyBP;MGC87971;PNAS 107;PNAS107;RP1 102G20.6;S100A6 binding protein;S100A6-binding protein;S100A6BP;Siah-interacting protein;SIP;

Immunogens

Immunogen:

A synthesized peptide derived from human CacyBP.

Uniprot:

Q9HB71(CYBP_HUMAN) >>Visit HPA database.

Sequence:

Q9HB71 CYBP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASEELQKDLEEVKVLLEKATRKRVRDALTAEKSKIETEIKNKMQQKSQKKAELLDNEKPAAVVAPITTGYTVKISNYGWDQSDKFVKIYITLTGVHQVPTENVQVHFTERSFDLLVKNLNGKSYSMIVNNLLKPISVEGSSKKVKTDTVLILCRKKVENTRWDYLTQVEKECKEKEKPSYDTETDPSEGLMNVLKKIYEDGDDDMKRTINKAWVESREKQAKGDTEF

Research Backgrounds

Q9HB71_CYBP_HUMAN

Function:

May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).

PTMs:

Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.

Subunit Structure:

Homodimer. Interacts with proteins of the S100 family S100A1, S100A6, S100B, S100P and S100A12 in a calcium-dependent manner (By similarity). Component of some large E3 complex at least composed of UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Interacts directly with SIAH1, SIAH2 and SKP1.

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CacyBP Antibody - #AF0017