PKA-R2 beta Antibody | Affinity Biosciences

Product:	PKA-R2 beta Antibody
Catalog:	AF6952
Description:	Rabbit polyclonal antibody to PKA-R2 beta
Application:	ELISA(peptide)
Reactivity:	Human
Mol.Wt.:	46kD(Calculated).
Uniprot:	P31323
RRID:	AB_2847742

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Secondary Antibodies
Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

PKA-R2 beta Antibody detects endogenous levels of total PKA-R2 beta.

RRID:

AB_2847742
Cite Format: Affinity Biosciences Cat# AF6952, RRID:AB_2847742.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AI451071; AW061005; cAMP dependent protein kinase type II beta regulatory chain; cAMP dependent protein kinase type II beta regulatory subunit; cAMP-dependent protein kinase type II-beta regulatory subunit; H RG363E19.2; KAP3_HUMAN; MGC116401; Pkarb2; PRKAR 2; PRKAR2; PRKAR2B; Protein kinase cAMP dependent regulatory type II beta; RATDNA; RII beta; RII(beta); RIIbeta; WUGSC:H RG363E19.2;

Immunogens

Immunogen:

A synthesized peptide derived from human PKA-R2 beta.

Uniprot:

P31323(KAP3_HUMAN) >>Visit HPA database.

Gene(ID):

PRKAR2B(5577)

Expression:

P31323 KAP3_HUMAN:

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Sequence:

P31323 KAP3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSIEIPAGLTELLQGFTVEVLRHQPADLLEFALQHFTRLQQENERKGTARFGHEGRTWGDLGAAAGGGTPSKGVNFAEEPMQSDSEDGEEEEAAPADAGAFNAPVINRFTRRASVCAEAYNPDEEEDDAESRIIHPKTDDQRNRLQEACKDILLFKNLDPEQMSQVLDAMFEKLVKDGEHVIDQGDDGDNFYVIDRGTFDIYVKCDGVGRCVGNYDNRGSFGELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEFSERLKVVDVIGTKVYNDGEQIIAQGDSADSFFIVESGEVKITMKRKGKSEVEENGAVEIARCSRGQYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVALFGTNMDIVEPTA

PTMs - P31323 As Substrate

P31323

Site	PTM Type	Enzyme	Source
T69	Phosphorylation		Uniprot
S71	Phosphorylation		Uniprot
S83	Phosphorylation		Uniprot
S85	Phosphorylation		Uniprot
S114	Phosphorylation	P31323 (PRKAR2B) , P17612 (PRKACA)	Uniprot
C116	S-Nitrosylation		Uniprot
Y120	Phosphorylation		Uniprot
K137	Ubiquitination		Uniprot
K150	Ubiquitination		Uniprot
T357	Phosphorylation		Uniprot
K359	Ubiquitination		Uniprot
K372	Ubiquitination		Uniprot
T417	Phosphorylation		Uniprot

PTMs - P31323 As Enzyme

P31323

Substrate	Site	Source
P31323 (PRKAR2B)	S114	Uniprot
Q14847-1 (LASP1)	S99	Uniprot
Q14847-1 (LASP1)	S146	Uniprot

Research Backgrounds

P31323_KAP3_HUMAN

Function:

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

PTMs:

Phosphorylated by the activated catalytic chain.

Subcellular Location:

Cytoplasm. Cell membrane.
Note: Colocalizes with PJA2 in the cytoplasm and at the cell membrane.

Tissue Specificity:

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Subunit Structure:

The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with the phosphorylated form of PJA2. Forms a complex composed of PRKAR2B, GSK3B and GSKIP through GSKIP interaction; facilitates PKA-induced phosphorylation and regulates GSK3B activity.

Family&Domains:

Belongs to the cAMP-dependent kinase regulatory chain family.

Research Fields

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

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PKA-R2 beta Antibody - #AF6952