Product: C/EBP epsilon Antibody
Catalog: AF6951
Description: Rabbit polyclonal antibody to C/EBP epsilon
Application: ELISA(peptide)
Reactivity: Human, Mouse, Rat
Mol.Wt.: 31kD(Calculated).
Uniprot: Q15744
RRID: AB_2847741

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
C/EBP epsilon Antibody detects endogenous levels of total C/EBP epsilon.
RRID:
AB_2847741
Cite Format: Affinity Biosciences Cat# AF6951, RRID:AB_2847741.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C/EBP epsilon; CCAAT/enhancer binding protein (C/EBP) epsilon; CCAAT/enhancer binding protein epsilon; CCAAT/enhancer-binding protein epsilon; CEBPE; CEBPE_HUMAN; CRP 1; CRP1;

Immunogens

Immunogen:

A synthesized peptide derived from human C/EBP epsilon.

Uniprot:
Gene(ID):
Expression:
Q15744 CEBPE_HUMAN:

Strongest expression occurs in promyelocyte and late-myeloblast-like cell lines.

Sequence:
MSHGTYYECEPRGGQQPLEFSGGRAGPGELGDMCEHEASIDLSAYIESGEEQLLSDLFAVKPAPEARGLKGPGTPAFPHYLPPDPRPFAYPPHTFGPDRKALGPGIYSSPGSYDPRAVAVKEEPRGPEGSRAASRGSYNPLQYQVAHCGQTAMHLPPTLAAPGQPLRVLKAPLATAAPPCSPLLKAPSPAGPLHKGKKAVNKDSLEYRLRRERNNIAVRKSRDKAKRRILETQQKVLEYMAENERLRSRVEQLTQELDTLRNLFRQIPEAANLIKGVGGCS

PTMs - Q15744 As Substrate

Site PTM Type Enzyme
T74 Phosphorylation
Y80 Phosphorylation
Y107 Phosphorylation
S108 Phosphorylation
S109 Phosphorylation
K121 Acetylation
K121 Sumoylation
S181 Phosphorylation
S188 Phosphorylation
K198 Acetylation
Y207 Phosphorylation

Research Backgrounds

Function:

Transcriptional activator. C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Required for the promyelocyte-myelocyte transition in myeloid differentiation.

PTMs:

Phosphorylated.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Strongest expression occurs in promyelocyte and late-myeloblast-like cell lines.

Subunit Structure:

Binds DNA as a homodimer and as a heterodimer. Can form stable heterodimers with CEBPA, CEBPB and CEBPD (By similarity). Interacts with GATA1 AND SPI1. Interacts with SMARCD2.

Family&Domains:

Belongs to the bZIP family. C/EBP subfamily.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.