C/EBP epsilon Antibody | Affinity Biosciences

Product:	C/EBP epsilon Antibody
Catalog:	AF6951
Description:	Rabbit polyclonal antibody to C/EBP epsilon
Application:	ELISA(peptide)
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	31kD(Calculated).
Uniprot:	Q15744
RRID:	AB_2847741

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

C/EBP epsilon Antibody detects endogenous levels of total C/EBP epsilon.

RRID:

AB_2847741
Cite Format: Affinity Biosciences Cat# AF6951, RRID:AB_2847741.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C/EBP epsilon; CCAAT/enhancer binding protein (C/EBP) epsilon; CCAAT/enhancer binding protein epsilon; CCAAT/enhancer-binding protein epsilon; CEBPE; CEBPE_HUMAN; CRP 1; CRP1;

Immunogens

Immunogen:

A synthesized peptide derived from human C/EBP epsilon.

Uniprot:

Q15744(CEBPE_HUMAN) >>Visit HPA database.

Gene(ID):

CEBPE(1053)

Expression:

Q15744 CEBPE_HUMAN:

Strongest expression occurs in promyelocyte and late-myeloblast-like cell lines.

Sequence:

Q15744 CEBPE_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSHGTYYECEPRGGQQPLEFSGGRAGPGELGDMCEHEASIDLSAYIESGEEQLLSDLFAVKPAPEARGLKGPGTPAFPHYLPPDPRPFAYPPHTFGPDRKALGPGIYSSPGSYDPRAVAVKEEPRGPEGSRAASRGSYNPLQYQVAHCGQTAMHLPPTLAAPGQPLRVLKAPLATAAPPCSPLLKAPSPAGPLHKGKKAVNKDSLEYRLRRERNNIAVRKSRDKAKRRILETQQKVLEYMAENERLRSRVEQLTQELDTLRNLFRQIPEAANLIKGVGGCS

PTMs - Q15744 As Substrate

Q15744

Site	PTM Type	Source
T74	Phosphorylation	Uniprot
Y80	Phosphorylation	Uniprot
Y107	Phosphorylation	Uniprot
S108	Phosphorylation	Uniprot
S109	Phosphorylation	Uniprot
K121	Acetylation	Uniprot
K121	Sumoylation	Uniprot
S181	Phosphorylation	Uniprot
S188	Phosphorylation	Uniprot
K198	Acetylation	Uniprot
Y207	Phosphorylation	Uniprot

Research Backgrounds

Q15744_CEBPE_HUMAN

Function:

Transcriptional activator. C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Required for the promyelocyte-myelocyte transition in myeloid differentiation.

PTMs:

Phosphorylated.

Subcellular Location:

Nucleus.

Tissue Specificity:

Strongest expression occurs in promyelocyte and late-myeloblast-like cell lines.

Subunit Structure:

Binds DNA as a homodimer and as a heterodimer. Can form stable heterodimers with CEBPA, CEBPB and CEBPD (By similarity). Interacts with GATA1 AND SPI1. Interacts with SMARCD2.

Family&Domains:

Belongs to the bZIP family. C/EBP subfamily.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

C/EBP epsilon Antibody - #AF6951