AOS1 Antibody - #AF0266

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Product: AOS1 Antibody
Catalog: AF0266
Description: Rabbit polyclonal antibody to AOS1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 38kDa; 38kD(Calculated).
Uniprot: Q9UBE0
RRID: AB_2833440

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(85%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(80%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
AOS1 Antibody detects endogenous levels of total AOS1.
RRID:
AB_2833440
Cite Format: Affinity Biosciences Cat# AF0266, RRID:AB_2833440.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Activator of SUMO1; AOS1; HSPC140; Sae1; SAE1_HUMAN; Sentrin/SUMO activating protein AOS1; SUA1; SUMO 1 activating enzyme E1 N subunit; SUMO 1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; Ubiquitin like protein SUMO1 activating enzyme; Ubiquitin-like 1-activating enzyme E1A; UBL E1A; UBLE1A;

Immunogens

Immunogen:
Uniprot:

Q9UBE0(SAE1_HUMAN) >>Visit HPA database.

Gene(ID):
SAE1(10055)
Expression:
Q9UBE0 SAE1_HUMAN:

Expression level increases during S phase and drops in G2 phase (at protein level).

Description:
UBLE1A The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2. Belongs to the ubiquitin-activating E1 family. Heterodimer of SAE1 and SAE2. The complex binds SUMO proteins via SAE2.
Sequence:
MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
92
Zebrafish
85
Chicken
80
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UBE0 As Substrate

Site PTM Type Enzyme
M1 Acetylation
V2 Acetylation
S12 Phosphorylation
R24 Methylation
K32 Ubiquitination
S37 Phosphorylation
K45 Ubiquitination
K53 Ubiquitination
K61 Ubiquitination
K109 Sumoylation
K109 Ubiquitination
K141 Ubiquitination
K148 Ubiquitination
S150 Phosphorylation
K178 Ubiquitination
K183 Ubiquitination
S185 Phosphorylation
K195 Acetylation
K195 Sumoylation
K195 Ubiquitination
K198 Acetylation
K198 Ubiquitination
S201 Phosphorylation
K208 Ubiquitination
K210 Ubiquitination
K217 Ubiquitination
K228 Acetylation
K228 Ubiquitination
Y262 Phosphorylation
S266 Phosphorylation
K335 Ubiquitination

Research Backgrounds

Function:

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expression level increases during S phase and drops in G2 phase (at protein level).

Subunit Structure:

Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1. Interacts with UBE2I.

Family&Domains:

Belongs to the ubiquitin-activating E1 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

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