UBF1 Antibody | Affinity Biosciences

Product:	UBF1 Antibody
Catalog:	AF6925
Description:	Rabbit polyclonal antibody to UBF1
Application:	ELISA(peptide)
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	89kD(Calculated).
Uniprot:	P17480
RRID:	AB_2847715

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Secondary Antibodies
Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

UBF1 Antibody detects endogenous levels of total UBF1.

RRID:

AB_2847715
Cite Format: Affinity Biosciences Cat# AF6925, RRID:AB_2847715.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

90 kDa nucleolus organizer region autoantigen; Autoantigen NOR-90; NOR 90; Nucleolar transcription factor 1; UBF 1; UBF; UBF-1; UBF1_HUMAN; Ubtf; Upstream binding factor 1; upstream binding transcription factor, RNA polymerase I; Upstream-binding factor 1;

Immunogens

Immunogen:

A synthesized peptide derived from human UBF1.

Uniprot:

P17480(UBF1_HUMAN) >>Visit HPA database.

Gene(ID):

UBTF(7343)

Sequence:

P17480 UBF1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNGEADCPTDLEMAAPKGQDRWSQEDMLTLLECMKNNLPSNDSSKFKTTESHMDWEKVAFKDFSGDMCKLKWVEISNEVRKFRTLTELILDAQEHVKNPYKGKKLKKHPDFPKKPLTPYFRFFMEKRAKYAKLHPEMSNLDLTKILSKKYKELPEKKKMKYIQDFQREKQEFERNLARFREDHPDLIQNAKKSDIPEKPKTPQQLWYTHEKKVYLKVRPDATTKEVKDSLGKQWSQLSDKKRLKWIHKALEQRKEYEEIMRDYIQKHPELNISEEGITKSTLTKAERQLKDKFDGRPTKPPPNSYSLYCAELMANMKDVPSTERMVLCSQQWKLLSQKEKDAYHKKCDQKKKDYEVELLRFLESLPEEEQQRVLGEEKMLNINKKQATSPASKKPAQEGGKGGSEKPKRPVSAMFIFSEEKRRQLQEERPELSESELTRLLARMWNDLSEKKKAKYKAREAALKAQSERKPGGEREERGKLPESPKRAEEIWQQSVIGDYLARFKNDRVKALKAMEMTWNNMEKKEKLMWIKKAAEDQKRYERELSEMRAPPAATNSSKKMKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKERMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHLDLWVKSLSPQDRAAYKEYISNKRKSMTKLRGPNPKSSRTTLQSKSESEEDDEEDEDDEDEDEEEEDDENGDSSEDGGDSSESSSEDESEDGDENEEDDEDEDDDEDDDEDEDNESEGSSSSSSSSGDSSDSDSN

PTMs - P17480 As Substrate

P17480

Site	PTM Type	Enzyme	Source
	Phosphorylation		Uniprot
M1	Acetylation		Uniprot
T9	Phosphorylation		Uniprot
K17	Ubiquitination		Uniprot
S23	Phosphorylation		Uniprot
K35	Ubiquitination		Uniprot
S44	Phosphorylation		Uniprot
K45	Ubiquitination		Uniprot
K47	Ubiquitination		Uniprot
K57	Ubiquitination		Uniprot
K61	Acetylation		Uniprot
K61	Ubiquitination		Uniprot
K69	Ubiquitination		Uniprot
K71	Ubiquitination		Uniprot
K97	Ubiquitination		Uniprot
K107	Ubiquitination		Uniprot
K114	Acetylation		Uniprot
K114	Sumoylation		Uniprot
K114	Ubiquitination		Uniprot
T117	Phosphorylation	P28482 (MAPK1) , P27361 (MAPK3)	Uniprot
K126	Ubiquitination		Uniprot
K132	Ubiquitination		Uniprot
K144	Ubiquitination		Uniprot
S147	Phosphorylation		Uniprot
Y150	Phosphorylation		Uniprot
K151	Ubiquitination		Uniprot
K160	Sumoylation		Uniprot
K160	Ubiquitination		Uniprot
K169	Ubiquitination		Uniprot
K191	Ubiquitination		Uniprot
K198	Ubiquitination		Uniprot
K200	Ubiquitination		Uniprot
T201	Phosphorylation	P28482 (MAPK1) , P27361 (MAPK3)	Uniprot
Y207	Phosphorylation		Uniprot
T208	Phosphorylation		Uniprot
K211	Ubiquitination		Uniprot
K216	Ubiquitination		Uniprot
K224	Ubiquitination		Uniprot
K232	Methylation		Uniprot
K232	Ubiquitination		Uniprot
S238	Phosphorylation		Uniprot
K240	Ubiquitination		Uniprot
K244	Ubiquitination		Uniprot
K248	Ubiquitination		Uniprot
K254	Methylation		Uniprot
K254	Ubiquitination		Uniprot
K266	Ubiquitination		Uniprot
S273	Phosphorylation		Uniprot
K279	Ubiquitination		Uniprot
K284	Acetylation		Uniprot
K284	Sumoylation		Uniprot
K284	Ubiquitination		Uniprot
S306	Phosphorylation		Uniprot
Y308	Phosphorylation		Uniprot
S329	Phosphorylation		Uniprot
K333	Ubiquitination		Uniprot
S336	Phosphorylation		Uniprot
K338	Ubiquitination		Uniprot
K352	Acetylation		Uniprot
K352	Ubiquitination		Uniprot
S364	Phosphorylation		Uniprot
K378	Ubiquitination		Uniprot
K384	Ubiquitination		Uniprot
K385	Acetylation		Uniprot
T388	Phosphorylation		Uniprot
S389	Phosphorylation	P24941 (CDK2)	Uniprot
S392	Phosphorylation		Uniprot
K394	Acetylation		Uniprot
K401	Acetylation		Uniprot
K406	Acetylation		Uniprot
S412	Phosphorylation		Uniprot
K421	Ubiquitination		Uniprot
R429	Methylation		Uniprot
S433	Phosphorylation		Uniprot
S435	Phosphorylation		Uniprot
R443	Methylation		Uniprot
S449	Phosphorylation		Uniprot
Y456	Phosphorylation		Uniprot
K464	Sumoylation		Uniprot
K480	Ubiquitination		Uniprot
S484	Phosphorylation	P24941 (CDK2) , P11802 (CDK4)	Uniprot
S495	Phosphorylation		Uniprot
Y500	Phosphorylation		Uniprot
S546	Phosphorylation		Uniprot
K559	Acetylation		Uniprot
S584	Phosphorylation		Uniprot
K594	Ubiquitination		Uniprot
S602	Phosphorylation		Uniprot
S610	Phosphorylation		Uniprot
K624	Ubiquitination		Uniprot
S638	Phosphorylation		Uniprot
R642	Methylation		Uniprot
K646	Ubiquitination		Uniprot
Y648	Phosphorylation		Uniprot
S655	Phosphorylation		Uniprot
S666	Phosphorylation		Uniprot

Research Backgrounds

P17480_UBF1_HUMAN

Function:

Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.

PTMs:

Phosphorylated and activated by PIK3CA.

Subcellular Location:

Nucleus>Nucleolus.

Subunit Structure:

Homodimer (By similarity). Interacts with TBP. Interacts with TAF1A. Interacts with RASL11A (By similarity). Binds to IRS1 and PIK3CA (By similarity). Interacts with DHX33. Interacts with PHF6. Interacts with CEBPA (isoform 1 and isoform 4). Interacts with DDX11. Interacts with NOP53.

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For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

UBF1 Antibody - #AF6925