CAD Antibody - #AF6916
(1)
| Product: | CAD Antibody |
| Catalog: | AF6916 |
| Description: | Rabbit polyclonal antibody to CAD |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Mol.Wt.: | 243kD(Calculated). |
| Uniprot: | P27708 |
| RRID: | AB_2847706 |
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
CAD Antibody detects endogenous levels of total CAD.
RRID:
AB_2847706
Cite Format: Affinity Biosciences Cat# AF6916, RRID:AB_2847706.
Cite Format: Affinity Biosciences Cat# AF6916, RRID:AB_2847706.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Aspartate carbamoyltransferase; Aspartate transcarbamylase; CAD protein; CAD trifunctional protein; Carbamoyl phosphate synthetase 2; Carbamoyl-phosphate synthetase 2 aspartate transcarbamylase and dihydroorotase; CPSase ATCase DHOase; Dihydroorotase; Glutamine dependent carbamoyl phosphate synthase; Multifunctional protein CAD;
Immunogens
Immunogen:
A synthesized peptide derived from human CAD.
Uniprot:
Gene(ID):
Sequence:
- P27708 PYR1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAALVLEDGSVLRGQPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPPDEMDEFGLCKWFESSGIHVAALVVGECCPTPSHWSATRTLHEWLQQHGIPGLQGVDTRELTKKLREQGSLLGKLVQNGTEPSSLPFLDPNARPLVPEVSIKTPRVFNTGGAPRILALDCGLKYNQIRCLCQRGAEVTVVPWDHALDSQEYEGLFLSNGPGDPASYPSVVSTLSRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLVGSGRCFLTSQNHGFAVETDSLPADWAPLFTNANDGSNEGIVHNSLPFFSVQFHPEHQAGPSDMELLFDIFLETVKEATAGNPGGQTVRERLTERLCPPGIPTPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITPHYVTQVIRNERPDGVLLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASNREELSALVAPAFAHTSQVLVDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGESIVVAPSQTLNDREYQLLRQTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPELRNSVTGGTAAFEPSVDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGRSFEEAFQKALRMVDENCVGFDHTVKPVSDMELETPTDKRIFVVAAALWAGYSVDRLYELTRIDRWFLHRMKRIIAHAQLLEQHRGQPLPPDLLQQAKCLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYWGTTHDLTFRTPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCQTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVASDGVVAAIAISEHVENAGVHSGDATLVTPPQDITAKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRVIMGEEVEPVGLMTGSGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPKKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILEQLAEKNFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGITMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFALFLGASSENAGTLGTVAGSAAGLKLYLNETFSELRLDSVVQWMEHFETWPSHLPIVAHAEQQTVAAVLMVAQLTQRSVHICHVARKEEILLIKAAKARGLPVTCEVAPHHLFLSHDDLERLGPGKGEVRPELGSRQDVEALWENMAVIDCFASDHAPHTLEEKCGSRPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPPQEDTYVEVDLEHEWTIPSHMPFSKAHWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGAVPQLPPSAPATSEMTTTPERPRRGIPGLPDGRFHLPPRIHRASDPGLPAEEPKEKSSRKVAEPELMGTPDGTCYPPPPVPRQASPQNLGTPGLLHPQTSPLLHSLVGQHILSVQQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPTVRAFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSTQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF
PTMs - P27708 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S10 | Phosphorylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| T166 | Phosphorylation | Uniprot | |
| T172 | Phosphorylation | Uniprot | |
| R177 | Methylation | Uniprot | |
| K186 | Acetylation | Uniprot | |
| K186 | Methylation | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| Y267 | Phosphorylation | Uniprot | |
| T375 | Phosphorylation | Uniprot | |
| K424 | Ubiquitination | Uniprot | |
| T456 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| T475 | Phosphorylation | Uniprot | |
| K490 | Ubiquitination | Uniprot | |
| T504 | Phosphorylation | Uniprot | |
| T585 | Phosphorylation | Uniprot | |
| S586 | Phosphorylation | Uniprot | |
| K592 | Ubiquitination | Uniprot | |
| K598 | Ubiquitination | Uniprot | |
| Y602 | Phosphorylation | Uniprot | |
| K696 | Ubiquitination | Uniprot | |
| Y735 | Phosphorylation | Uniprot | |
| K739 | Ubiquitination | Uniprot | |
| K747 | Acetylation | Uniprot | |
| K747 | Ubiquitination | Uniprot | |
| K754 | Ubiquitination | Uniprot | |
| K760 | Ubiquitination | Uniprot | |
| S761 | Phosphorylation | Uniprot | |
| K778 | Ubiquitination | Uniprot | |
| K795 | Ubiquitination | Uniprot | |
| K808 | Ubiquitination | Uniprot | |
| K867 | Ubiquitination | Uniprot | |
| K874 | Ubiquitination | Uniprot | |
| T883 | Phosphorylation | Uniprot | |
| T1037 | Phosphorylation | P27708 (CAD) | Uniprot |
| S1038 | Phosphorylation | Uniprot | |
| S1044 | Phosphorylation | Uniprot | |
| S1061 | Phosphorylation | Uniprot | |
| S1069 | Phosphorylation | Uniprot | |
| S1073 | Phosphorylation | Uniprot | |
| S1112 | Phosphorylation | Uniprot | |
| K1119 | Ubiquitination | Uniprot | |
| K1127 | Ubiquitination | Uniprot | |
| K1177 | Ubiquitination | Uniprot | |
| K1206 | Ubiquitination | Uniprot | |
| K1211 | Ubiquitination | Uniprot | |
| S1220 | Phosphorylation | Uniprot | |
| S1222 | Phosphorylation | Uniprot | |
| K1228 | Ubiquitination | Uniprot | |
| K1262 | Ubiquitination | Uniprot | |
| S1267 | Phosphorylation | Uniprot | |
| K1301 | Methylation | Uniprot | |
| K1309 | Ubiquitination | Uniprot | |
| K1313 | Ubiquitination | Uniprot | |
| K1323 | Ubiquitination | Uniprot | |
| K1325 | Ubiquitination | Uniprot | |
| K1387 | Ubiquitination | Uniprot | |
| S1406 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S1407 | Phosphorylation | Uniprot | |
| K1411 | Acetylation | Uniprot | |
| K1411 | Ubiquitination | Uniprot | |
| S1423 | Phosphorylation | Uniprot | |
| K1431 | Ubiquitination | Uniprot | |
| K1434 | Ubiquitination | Uniprot | |
| K1450 | Sumoylation | Uniprot | |
| K1450 | Ubiquitination | Uniprot | |
| K1460 | Ubiquitination | Uniprot | |
| K1521 | Ubiquitination | Uniprot | |
| K1618 | Ubiquitination | Uniprot | |
| K1625 | Ubiquitination | Uniprot | |
| S1646 | Phosphorylation | Uniprot | |
| K1657 | Ubiquitination | Uniprot | |
| S1698 | Phosphorylation | Uniprot | |
| S1724 | Phosphorylation | Uniprot | |
| K1778 | Ubiquitination | Uniprot | |
| Y1794 | Phosphorylation | Uniprot | |
| Y1805 | Phosphorylation | Uniprot | |
| S1823 | Phosphorylation | Uniprot | |
| T1827 | Phosphorylation | Uniprot | |
| S1828 | Phosphorylation | Uniprot | |
| T1831 | Phosphorylation | Uniprot | |
| T1832 | Phosphorylation | Uniprot | |
| T1833 | Phosphorylation | Uniprot | |
| R1839 | Methylation | Uniprot | |
| H1856 | Phosphorylation | Uniprot | |
| S1859 | Phosphorylation | P23443 (RPS6KB1) , P17612 (PRKACA) | Uniprot |
| S1873 | Phosphorylation | Uniprot | |
| T1884 | Phosphorylation | Uniprot | |
| T1888 | Phosphorylation | Uniprot | |
| C1889 | S-Nitrosylation | Uniprot | |
| Y1890 | Phosphorylation | Uniprot | |
| S1900 | Phosphorylation | Uniprot | |
| T1906 | Phosphorylation | Uniprot | |
| T1914 | Phosphorylation | Uniprot | |
| S1920 | Phosphorylation | Uniprot | |
| S1938 | Phosphorylation | Uniprot | |
| K1953 | Ubiquitination | Uniprot | |
| K1961 | Ubiquitination | Uniprot | |
| K2036 | Ubiquitination | Uniprot | |
| Y2103 | Phosphorylation | Uniprot | |
| K2125 | Ubiquitination | Uniprot | |
| S2154 | Phosphorylation | Uniprot | |
| T2155 | Phosphorylation | Uniprot | |
| Y2158 | Phosphorylation | Uniprot | |
| K2178 | Ubiquitination | Uniprot | |
| Y2203 | Phosphorylation | Uniprot | |
| Y2212 | Phosphorylation | Uniprot | |
| T2220 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
This protein is a 'fusion' protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).
PTMs:
Activated by MAP kinase (Erk1/2) phosphorylation just prior to the S phase of the cell cycle, when the demand for pyrimidine nucleotides is greatest, and down-regulated as the cells emerge from S phase by protein kinase A (PKA) phosphorylation. Phosphorylation at Ser-1859 by RPS6KB1 downstream of MTOR promotes oligomerization and stimulates dihydroorotase activity. Phosphorylation at Ser-1406 reduces sensitivity to feedback inhibition by UTP.
Subcellular Location:
Cytoplasm. Nucleus.
Note: Cytosolic and unphosphorylated in resting cells, translocates to the nucleus in response to EGF stimulation, nuclear import promotes optimal cell growth.
Subunit Structure:
Homohexamer. Interacts with CIPC.
Family&Domains:
In the central section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily.
Research Fields
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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