Phospho-p63 (Ser395) Antibody | Affinity Biosciences

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AF3976 at 1/100 staining Mouse spleen tissue by IHC-P.

Product:	Phospho-p63 (Ser395) Antibody
Catalog:	AF3976
Description:	Rabbit polyclonal antibody to Phospho-p63 (Ser395)
Application:	IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	77kD(Calculated).
Uniprot:	Q9H3D4
RRID:	AB_2847699

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-p63 (Ser395) Antibody detects endogenous levels of p63 only when phosphorylated at Ser395.

RRID:

AB_2847699
Cite Format: Affinity Biosciences Cat# AF3976, RRID:AB_2847699.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AIS; Amplified in squamous cell carcinoma; B(p51A); B(p51B); Chronic ulcerative stomatitis protein; CUSP; DN p63 alpha 1; DNp63; EEC3; id:ibd3516; Keratinocyte transcription factor; Keratinocyte transcription factor KET; KET; LMS; MGC115972; MGC192897; NBP; OFC8; OTTHUMP00000209732; OTTHUMP00000209733; OTTHUMP00000209734; OTTHUMP00000209735; OTTHUMP00000209737; OTTHUMP00000209738; OTTHUMP00000209739; OTTHUMP00000209740; OTTHUMP00000209741; OTTHUMP00000209742; OTTHUMP00000209743; OTTHUMP00000209744; p40; p51; P51/P63; p53-related protein p63; p53CP; p63; P63_HUMAN; p73H; p73L; RHS; SHFM4; TAp63alpha; TP53CP; TP53L; TP63; TP73L; Transformation related protein 63; Transformation-related protein 63; Trp53rp1; Trp63; Tumor protein 63; Tumor protein p53-competing protein; Tumor protein p53-like; Tumor protein p63; Tumor protein p63 deltaN isoform delta; Tumor protein p73; Tumor protein p73-like;

Immunogens

Immunogen:

A synthesized peptide derived from human p63 around the phosphorylation site of Ser395.

Uniprot:

Q9H3D4(P63_HUMAN) >>Visit HPA database.

Gene(ID):

TP63(8626)

Expression:

Q9H3D4 P63_HUMAN:

Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.

Sequence:

Q9H3D4 P63_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNFETSRCATLQYCPDPYIQRFVETPAHFSWKESYYRSTMSQSTQTNEFLSPEVFQHIWDFLEQPICSVQPIDLNFVDEPSEDGATNKIEISMDCIRMQDSDLSDPMWPQYTNLGLLNSMDQQIQNGSSSTSPYNTDHAQNSVTAPSPYAQPSSTFDALSPSPAIPSNTDYPGPHSFDVSFQQSSTAKSATWTYSTELKKLYCQIAKTCPIQIKVMTPPPQGAVIRAMPVYKKAEHVTEVVKRCPNHELSREFNEGQIAPPSHLIRVEGNSHAQYVEDPITGRQSVLVPYEPPQVGTEFTTVLYNFMCNSSCVGGMNRRPILIIVTLETRDGQVLGRRCFEARICACPGRDRKADEDSIRKQQVSDSTKNGDGTKRPFRQNTHGIQMTSIKKRRSPDDELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQHQHLLQKQTSIQSPSSYGNSSPPLNKMNSMNKLPSVSQLINPQQRNALTPTTIPDGMGANIPMMGTHMPMAGDMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCSIVSFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQLHEFSSPSHLLRTPSSASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDARRNKQQRIKEEGE

PTMs - Q9H3D4 As Substrate

Q9H3D4

Site	PTM Type	Enzyme	Source
Y13	Phosphorylation		Uniprot
Y18	Phosphorylation		Uniprot
Y36	Phosphorylation		Uniprot
S43	Phosphorylation	O14920 (IKBKB)	Uniprot
T46	Phosphorylation		Uniprot
S51	Phosphorylation	O14920 (IKBKB)	Uniprot
S68	Phosphorylation	P36897 (TGFBR1)	Uniprot
Y149	Phosphorylation	P00519 (ABL1)	Uniprot
S160	Phosphorylation	P36897 (TGFBR1)	Uniprot
S162	Phosphorylation		Uniprot
Y171	Phosphorylation	P00519 (ABL1)	Uniprot
Y231	Phosphorylation		Uniprot
K232	Acetylation		Uniprot
K242	Methylation		Uniprot
T281	Phosphorylation		Uniprot
Y290	Phosphorylation	P00519 (ABL1)	Uniprot
T326	Phosphorylation		Uniprot
T329	Phosphorylation		Uniprot
S358	Phosphorylation		Uniprot
S395	Phosphorylation		Uniprot
T451	Phosphorylation		Uniprot
S462	Phosphorylation		Uniprot
S463	Phosphorylation		Uniprot
S477	Phosphorylation	P23443 (RPS6KB1) , Q13315 (ATM) , P24941 (CDK2)	Uniprot
S479	Phosphorylation	Q13315 (ATM)	Uniprot
T491	Phosphorylation	Q13315 (ATM) , P24941 (CDK2) , P23443 (RPS6KB1) , Q9H2X6 (HIPK2)	Uniprot
S560	Phosphorylation	Q13315 (ATM) , P23443 (RPS6KB1) , P24941 (CDK2)	Uniprot
K588	Sumoylation		Uniprot
K599	Ubiquitination		Uniprot
K676	Sumoylation		Uniprot

Research Backgrounds

Q9H3D4_P63_HUMAN

Function:

Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter.

PTMs:

May be sumoylated.

Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.

Subcellular Location:

Nucleus.

Tissue Specificity:

Subunit Structure:

Binds DNA as a homotetramer. Isoform composition of the tetramer may determine transactivation activity. Isoforms Alpha and Gamma interact with HIPK2. Interacts with SSRP1, leading to stimulate coactivator activity. Isoform 1 and isoform 2 interact with WWP1. Interacts with PDS5A. Isoform 5 (via activation domain) interacts with NOC2L.

Family&Domains:

The transactivation inhibitory domain (TID) can interact with, and inhibit the activity of the N-terminal transcriptional activation domain of TA*-type isoforms.

Belongs to the p53 family.

Research Fields

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

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Phospho-p63 (Ser395) Antibody - #AF3976