Product: Phospho-p63 (Ser395) Antibody
Catalog: AF3976
Description: Rabbit polyclonal antibody to Phospho-p63 (Ser395)
Application: IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 77kD(Calculated).
Uniprot: Q9H3D4
RRID: AB_2847699

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-p63 (Ser395) Antibody detects endogenous levels of p63 only when phosphorylated at Ser395.
RRID:
AB_2847699
Cite Format: Affinity Biosciences Cat# AF3976, RRID:AB_2847699.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AIS; Amplified in squamous cell carcinoma; B(p51A); B(p51B); Chronic ulcerative stomatitis protein; CUSP; DN p63 alpha 1; DNp63; EEC3; id:ibd3516; Keratinocyte transcription factor; Keratinocyte transcription factor KET; KET; LMS; MGC115972; MGC192897; NBP; OFC8; OTTHUMP00000209732; OTTHUMP00000209733; OTTHUMP00000209734; OTTHUMP00000209735; OTTHUMP00000209737; OTTHUMP00000209738; OTTHUMP00000209739; OTTHUMP00000209740; OTTHUMP00000209741; OTTHUMP00000209742; OTTHUMP00000209743; OTTHUMP00000209744; p40; p51; P51/P63; p53-related protein p63; p53CP; p63; P63_HUMAN; p73H; p73L; RHS; SHFM4; TAp63alpha; TP53CP; TP53L; TP63; TP73L; Transformation related protein 63; Transformation-related protein 63; Trp53rp1; Trp63; Tumor protein 63; Tumor protein p53-competing protein; Tumor protein p53-like; Tumor protein p63; Tumor protein p63 deltaN isoform delta; Tumor protein p73; Tumor protein p73-like;

Immunogens

Immunogen:

A synthesized peptide derived from human p63 around the phosphorylation site of Ser395.

Uniprot:
Gene(ID):
Expression:
Q9H3D4 P63_HUMAN:

Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.

Sequence:
MNFETSRCATLQYCPDPYIQRFVETPAHFSWKESYYRSTMSQSTQTNEFLSPEVFQHIWDFLEQPICSVQPIDLNFVDEPSEDGATNKIEISMDCIRMQDSDLSDPMWPQYTNLGLLNSMDQQIQNGSSSTSPYNTDHAQNSVTAPSPYAQPSSTFDALSPSPAIPSNTDYPGPHSFDVSFQQSSTAKSATWTYSTELKKLYCQIAKTCPIQIKVMTPPPQGAVIRAMPVYKKAEHVTEVVKRCPNHELSREFNEGQIAPPSHLIRVEGNSHAQYVEDPITGRQSVLVPYEPPQVGTEFTTVLYNFMCNSSCVGGMNRRPILIIVTLETRDGQVLGRRCFEARICACPGRDRKADEDSIRKQQVSDSTKNGDGTKRPFRQNTHGIQMTSIKKRRSPDDELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQHQHLLQKQTSIQSPSSYGNSSPPLNKMNSMNKLPSVSQLINPQQRNALTPTTIPDGMGANIPMMGTHMPMAGDMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCSIVSFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQLHEFSSPSHLLRTPSSASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDARRNKQQRIKEEGE

PTMs - Q9H3D4 As Substrate

Site PTM Type Enzyme
Y13 Phosphorylation
Y18 Phosphorylation
Y36 Phosphorylation
S43 Phosphorylation O14920 (IKBKB)
T46 Phosphorylation
S51 Phosphorylation O14920 (IKBKB)
S68 Phosphorylation P36897 (TGFBR1)
Y149 Phosphorylation P00519 (ABL1)
S160 Phosphorylation P36897 (TGFBR1)
S162 Phosphorylation
Y171 Phosphorylation P00519 (ABL1)
Y231 Phosphorylation
K232 Acetylation
K242 Methylation
T281 Phosphorylation
Y290 Phosphorylation P00519 (ABL1)
T326 Phosphorylation
T329 Phosphorylation
S358 Phosphorylation
S395 Phosphorylation
T451 Phosphorylation
S462 Phosphorylation
S463 Phosphorylation
S477 Phosphorylation P23443 (RPS6KB1) , Q13315 (ATM) , P24941 (CDK2)
S479 Phosphorylation Q13315 (ATM)
T491 Phosphorylation Q13315 (ATM) , P24941 (CDK2) , P23443 (RPS6KB1) , Q9H2X6 (HIPK2)
S560 Phosphorylation Q13315 (ATM) , P23443 (RPS6KB1) , P24941 (CDK2)
K588 Sumoylation
K599 Ubiquitination
K676 Sumoylation

Research Backgrounds

Function:

Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter.

PTMs:

May be sumoylated.

Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.

Subunit Structure:

Binds DNA as a homotetramer. Isoform composition of the tetramer may determine transactivation activity. Isoforms Alpha and Gamma interact with HIPK2. Interacts with SSRP1, leading to stimulate coactivator activity. Isoform 1 and isoform 2 interact with WWP1. Interacts with PDS5A. Isoform 5 (via activation domain) interacts with NOC2L.

Family&Domains:

The transactivation inhibitory domain (TID) can interact with, and inhibit the activity of the N-terminal transcriptional activation domain of TA*-type isoforms.

Belongs to the p53 family.

Research Fields

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

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