Phospho-PEA15 (Ser104) Antibody | Affinity Biosciences

1/4

AF3963 at 1/100 staining Human ovarian cancer by IHC-P.

AF3963 at 1/100 staining Mouse liver tissue by IHC-P.

AF3963 at 1/100 staining Mouse kidney tissue by IHC-P.

AF3963 at 1/100 staining Rat pancreatic tissue by IHC-P.

Product:	Phospho-PEA15 (Ser104) Antibody
Catalog:	AF3963
Description:	Rabbit polyclonal antibody to Phospho-PEA15 (Ser104)
Application:	IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	15kD(Calculated).
Uniprot:	Q15121
RRID:	AB_2847686

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-PEA15 (Ser104) Antibody detects endogenous levels of PEA15 only when phosphorylated at Ser104.

RRID:

AB_2847686
Cite Format: Affinity Biosciences Cat# AF3963, RRID:AB_2847686.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

15 kDa phosphoprotein enriched in astrocytes; Astrocytic phosphoprotein PEA 15; Astrocytic phosphoprotein PEA-15; Astrocytic phosphoprotein PEA15; HMAT 1; HMAT1; Homolog of mouse MAT 1 oncogene; Homolog of mouse MAT1 oncogene; HUMMAT 1H; HUMMAT1H; MAT 1; MAT 1H; MAT1; MAT1H; PEA 15; Pea15; PEA15 protein; PEA15_HUMAN; PED; Phosphoprotein enriched in astrocytes 15; Phosphoprotein enriched in astrocytes 15kD; Phosphoprotein enriched in diabetes;

Immunogens

Immunogen:

A synthesized peptide derived from human PEA15 around the phosphorylation site of Ser104.

Uniprot:

Q15121(PEA15_HUMAN) >>Visit HPA database.

Gene(ID):

PEA15(8682)

Expression:

Q15121 PEA15_HUMAN:

Ubiquitously expressed. Most abundant in tissues such as heart, brain, muscle and adipose tissue which utilize glucose as an energy source. Lower expression in glucose-producing tissues. Higher levels of expression are found in tissues from individuals with type 2 diabetes than in controls.

Sequence:

Q15121 PEA15_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAEYGTLLQDLTNNITLEDLEQLKSACKEDIPSEKSEEITTGSAWFSFLESHNKLDKDNLSYIEHIFEISRRPDLLTMVVDYRTRVLKISEEDELDTKLTRIPSAKKYKDIIRQPSEEEIIKLAPPPKKA

PTMs - Q15121 As Substrate

Q15121

Site	PTM Type	Enzyme	Source
Y4	Phosphorylation		Uniprot
T6	Phosphorylation		Uniprot
K24	Ubiquitination		Uniprot
S25	Phosphorylation		Uniprot
C27	S-Nitrosylation		Uniprot
K28	Ubiquitination		Uniprot
S43	Phosphorylation		Uniprot
K54	Ubiquitination		Uniprot
S61	Phosphorylation		Uniprot
S90	Phosphorylation		Uniprot
T97	Phosphorylation		Uniprot
K98	Ubiquitination		Uniprot
T100	Phosphorylation		Uniprot
S104	Phosphorylation	P17252 (PRKCA)	Uniprot
Y108	Phosphorylation		Uniprot
K109	Methylation		Uniprot
S116	Phosphorylation	P31749 (AKT1) , Q9UQM7 (CAMK2A) , P54646 (PRKAA2)	Uniprot

Research Backgrounds

Q15121_PEA15_HUMAN

Function:

Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm (By similarity). Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.

PTMs:

Phosphorylated by protein kinase C and calcium-calmodulin-dependent protein kinase. These phosphorylation events are modulated by neurotransmitters or hormones.

Subcellular Location:

Cytoplasm.
Note: Associated with microtubules.

Tissue Specificity:

Subunit Structure:

Binds RPS6KA3, MAPK3 and MAPK1. Transient interaction with PLD1 and PLD2 (By similarity). Interacts with CASP8 and FADD.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-PEA15 (Ser104) Antibody - #AF3963