Phospho-Tal1 (Ser122) Antibody - #AF3958
| Product: | Phospho-Tal1 (Ser122) Antibody |
| Catalog: | AF3958 |
| Description: | Rabbit polyclonal antibody to Phospho-Tal1 (Ser122) |
| Application: | ELISA(peptide) |
| Reactivity: | Human, Mouse |
| Mol.Wt.: | 34kD(Calculated). |
| Uniprot: | P17542 |
| RRID: | AB_2847681 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF3958, RRID:AB_2847681.
Fold/Unfold
bHLHa17; Class A basic helix-loop-helix protein 17; OTTHUMP00000009563; OTTHUMP00000009564; SCL; STEM CELL LEUKEMIA HEMATOPOIETIC TRANSCRIPTION FACTOR; Stem cell protein; T cell acute lymphocytic leukemia 1; T cell acute lymphocytic leukemia 1 protein; T cell acute lymphocytic leukemia 1 protein; T cell leukemia/lymphoma 5 protein; T-cell acute lymphocytic leukemia protein 1; T-cell leukemia/lymphoma protein 5; Tal 1; Tal 1 product; TAL 1 protein; TAL bHLH transcription factor 1 erythroid differentiation factor; TAL-1; tal1; TAL1_HUMAN; TCL 5; TCL5;
Immunogens
A synthesized peptide derived from human Tal1 around the phosphorylation site of Ser122.
- P17542 TAL1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTERPPSEAARSDPQLEGRDAAEASMAPPHLVLLNGVAKETSRAAAAEPPVIELGARGGPGGGPAGGGGAARDLKGRDAATAEARHRVPTTELCRPPGPAPAPAPASVTAELPGDGRMVQLSPPALAAPAAPGRALLYSLSQPLASLGSGFFGEPDAFPMFTTNNRVKRRPSPYEMEITDGPHTKVVRRIFTNSRERWRQQNVNGAFAELRKLIPTHPPDKKLSKNEILRLAMKYINFLAKLLNDQEEEGTQRAKTGKDPVVGAGGGGGGGGGGAPPDDLLQDVLSPNSSCGSSLDGAASPDSYTEEPAPKHTARSLHPAMLPAADGAGPR
PTMs - P17542 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S7 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| T90 | Phosphorylation | P31749 (AKT1) | Uniprot |
| T91 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| S122 | Phosphorylation | P27361 (MAPK3) , Q02750 (MAP2K1) , P17612 (PRKACA) | Uniprot |
| Y138 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K212 | Acetylation | Uniprot | |
| T216 | Phosphorylation | Uniprot | |
| K221 | Acetylation | Uniprot | |
| K222 | Acetylation | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K225 | Acetylation | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| S316 | Phosphorylation | Uniprot |
Research Backgrounds
Implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation (By similarity).
Phosphorylated on serine residues. Phosphorylation of Ser-122 is strongly stimulated by hypoxia (By similarity).
Ubiquitinated; subsequent to hypoxia-dependent phosphorylation of Ser-122, ubiquitination targets the protein for rapid degradation via the ubiquitin system. This process may be characteristic for microvascular endothelial cells, since it could not be observed in large vessel endothelial cells (By similarity).
Nucleus.
Leukemic stem cell.
Efficient DNA binding requires dimerization with another bHLH protein. Forms heterodimers with TCF3. Binds to the LIM domain containing protein LMO2 and to DRG1. Can assemble in a complex with LDB1 and LMO2. Component of a TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3. Interacts with SBNO2; this interaction inhibits TAL1 occupancy of the DCSTAMP promoter, leading to the activation of the DCSTAMP promoter by the transcription factor MITF.
The helix-loop-helix domain is necessary and sufficient for the interaction with DRG1.
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