Product: Phospho-BLK (Tyr501) Antibody
Catalog: AF3955
Description: Rabbit polyclonal antibody to Phospho-BLK (Tyr501)
Application: ELISA(peptide)
Reactivity: Human
Mol.Wt.: 58kD(Calculated).
Uniprot: P51451
RRID: AB_2847678

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 100ul $350 In stock
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Product Info

Source:
Rabbit
Application:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
Phospho-BLK (Tyr501) Antibody detects endogenous levels of BLK only when phosphorylated at Tyr501.
RRID:
AB_2847678
Cite Format: Affinity Biosciences Cat# AF3955, RRID:AB_2847678.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B lymphocyte kinase; B lymphoid tyrosine kinase; Blk; BLK nonreceptor tyrosine kinase; BLK_HUMAN; EC 2.7.10.2; MGC 10442; MGC10442; MODY 11; MODY11; P55 BLK; p55-BLK; Tyrosine kinase B lymphocyte specific; Tyrosine protein kinase; Tyrosine-protein kinase BLK;

Immunogens

Immunogen:

A synthesized peptide derived from human BLK around the phosphorylation site of Tyr501.

Uniprot:
Gene(ID):
Expression:
P51451 BLK_HUMAN:

Expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles.

Sequence:
MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPPPPDEHLDEDKHFVVALYDYTAMNDRDLQMLKGEKLQVLKGTGDWWLARSLVTGREGYVPSNFVARVESLEMERWFFRSQGRKEAERQLLAPINKAGSFLIRESETNKGAFSLSVKDVTTQGELIKHYKIRCLDEGGYYISPRITFPSLQALVQHYSKKGDGLCQRLTLPCVRPAPQNPWAQDEWEIPRQSLRLVRKLGSGQFGEVWMGYYKNNMKVAIKTLKEGTMSPEAFLGEANVMKALQHERLVRLYAVVTKEPIYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQIAEGMAYIERMNSIHRDLRAANILVSEALCCKIADFGLARIIDSEYTAQEGAKFPIKWTAPEAIHFGVFTIKADVWSFGVLLMEVVTYGRVPYPGMSNPEVIRNLERGYRMPRPDTCPPELYRGVIAECWRSRPEERPTFEFLQSVLEDFYTATERQYELQP

PTMs - P51451 As Substrate

Site PTM Type Enzyme
S24 Phosphorylation
Y107 Phosphorylation
K144 Ubiquitination
K157 Ubiquitination
K165 Ubiquitination
K175 Ubiquitination
Y187 Phosphorylation
Y188 Phosphorylation
S197 Phosphorylation
Y205 Phosphorylation
K208 Ubiquitination
K272 Ubiquitination
K305 Ubiquitination
Y309 Phosphorylation
Y314 Phosphorylation
K325 Ubiquitination
S341 Phosphorylation
Y350 Phosphorylation
S356 Phosphorylation
K375 Ubiquitination
Y389 Phosphorylation
K396 Ubiquitination
Y494 Phosphorylation
Y501 Phosphorylation

PTMs - P51451 As Enzyme

Substrate Site Source
P11912 (CD79A) Y188 Uniprot
P11912 (CD79A) Y199 Uniprot
P12318-2 (FCGR2A) Y280 Uniprot
P12318 (FCGR2A) Y281 Uniprot
P12318-2 (FCGR2A) Y287 Uniprot
P12318 (FCGR2A) Y288 Uniprot
P12318-2 (FCGR2A) Y303 Uniprot
P12318 (FCGR2A) Y304 Uniprot
P31994-5 (FCGR2B) Y272 Uniprot
P31994-2 (FCGR2B) Y273 Uniprot
P31994-4 (FCGR2B) Y291 Uniprot
P31994 (FCGR2B) Y292 Uniprot
P31995 (FCGR2C) Y294 Uniprot
P31995 (FCGR2C) Y310 Uniprot
P40259 (CD79B) Y196 Uniprot
P40259 (CD79B) Y207 Uniprot

Research Backgrounds

Function:

Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling (By similarity). B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors (By similarity). Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation (By similarity). Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor (By similarity). Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr-207' (By similarity). Phosphorylates also the immunoglobulin G receptors FCGR2A, FCGR2B and FCGR2C. With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation (By similarity). Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation (By similarity). In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose. Phosphorylates CGAS, promoting retention of CGAS in the cytosol.

PTMs:

Phosphorylated on tyrosine residues after antibody-mediated surface engagement of the B-cell antigen receptor (BCR).

Ubiquitination of activated BLK by the UBE3A ubiquitin protein ligase leads to its degradation by the ubiquitin-proteasome pathway.

Subcellular Location:

Cell membrane>Lipid-anchor.
Note: Present and active in lipid rafts. Membrane location is required for the phosphorylation of CD79A and CD79B (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles.

Subunit Structure:

Interacts with CBL (via SH2 domain). Interacts with CD79A and CD79B (via SH2 domain) (By similarity).

Family&Domains:

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Restrictive clause

 

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For Research Use Only.
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