Phospho-PKA-R2 beta (Ser114) Antibody - #AF3952
| Product: | Phospho-PKA-R2 beta (Ser114) Antibody |
| Catalog: | AF3952 |
| Description: | Rabbit polyclonal antibody to Phospho-PKA-R2 beta (Ser114) |
| Application: | IHC |
| Reactivity: | Human, Mouse, Rat |
| Mol.Wt.: | 46kD(Calculated). |
| Uniprot: | P31323 |
| RRID: | AB_2847675 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF3952, RRID:AB_2847675.
Fold/Unfold
AI451071; AW061005; cAMP dependent protein kinase type II beta regulatory chain; cAMP dependent protein kinase type II beta regulatory subunit; cAMP-dependent protein kinase type II-beta regulatory subunit; H RG363E19.2; KAP3_HUMAN; MGC116401; Pkarb2; PRKAR 2; PRKAR2; PRKAR2B; Protein kinase cAMP dependent regulatory type II beta; RATDNA; RII beta; RII(beta); RIIbeta; WUGSC:H RG363E19.2;
Immunogens
A synthesized peptide derived from human PKA-R2 beta around the phosphorylation site of Ser114.
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
- P31323 KAP3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSIEIPAGLTELLQGFTVEVLRHQPADLLEFALQHFTRLQQENERKGTARFGHEGRTWGDLGAAAGGGTPSKGVNFAEEPMQSDSEDGEEEEAAPADAGAFNAPVINRFTRRASVCAEAYNPDEEEDDAESRIIHPKTDDQRNRLQEACKDILLFKNLDPEQMSQVLDAMFEKLVKDGEHVIDQGDDGDNFYVIDRGTFDIYVKCDGVGRCVGNYDNRGSFGELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEFSERLKVVDVIGTKVYNDGEQIIAQGDSADSFFIVESGEVKITMKRKGKSEVEENGAVEIARCSRGQYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVALFGTNMDIVEPTA
PTMs - P31323 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T69 | Phosphorylation | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | P31323 (PRKAR2B) , P17612 (PRKACA) | Uniprot |
| C116 | S-Nitrosylation | Uniprot | |
| Y120 | Phosphorylation | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| T357 | Phosphorylation | Uniprot | |
| K359 | Ubiquitination | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| T417 | Phosphorylation | Uniprot |
PTMs - P31323 As Enzyme
Research Backgrounds
Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Phosphorylated by the activated catalytic chain.
Cytoplasm. Cell membrane.
Note: Colocalizes with PJA2 in the cytoplasm and at the cell membrane.
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with the phosphorylated form of PJA2. Forms a complex composed of PRKAR2B, GSK3B and GSKIP through GSKIP interaction; facilitates PKA-induced phosphorylation and regulates GSK3B activity.
Belongs to the cAMP-dependent kinase regulatory chain family.
Research Fields
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
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