Phospho-Blooms Syndrome Protein Blm (Thr99) Antibody

Product:	Phospho-Blooms Syndrome Protein Blm (Thr99) Antibody
Catalog:	AF3949
Description:	Rabbit polyclonal antibody to Phospho-Blooms Syndrome Protein Blm (Thr99)
Application:	ELISA(peptide)
Reactivity:	Human
Mol.Wt.:	159kD(Calculated).
Uniprot:	P54132
RRID:	AB_2847672

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

Phospho-Blooms Syndrome Protein Blm (Thr99) Antibody detects endogenous levels of Blooms Syndrome Protein Blm only when phosphorylated at Thr99.

RRID:

AB_2847672
Cite Format: Affinity Biosciences Cat# AF3949, RRID:AB_2847672.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Blm; BLM_HUMAN; Bloom syndrome; Bloom syndrome protein; Bloom syndrome RecQ helicase like; BS; DNA helicase; DNA helicase RecQ like type 2; MGC126616; MGC131618; MGC131620; RECQ 2; RECQ like; RecQ like type 2; RecQ protein like 3; RecQ protein-like 3; RecQ-like type 2; RecQ2; RECQL 2; RECQL 3; RECQL2; RECQL3; type 2;

Immunogens

Immunogen:

A synthesized peptide derived from human Blooms Syndrome Protein Blm around the phosphorylation site of Thr99.

Uniprot:

P54132(BLM_HUMAN) >>Visit HPA database.

Gene(ID):

BLM(641)

Sequence:

P54132 BLM_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSDNNVSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRGGSKSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDFDTSETSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQIDLTEEQKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLEEAELHSTEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDVLSTSKDLLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS

PTMs - P54132 As Substrate

P54132

Site	PTM Type	Enzyme	Source
	Phosphorylation		Uniprot
S17	Phosphorylation		Uniprot
T20	Phosphorylation		Uniprot
K24	Sumoylation		Uniprot
K24	Ubiquitination		Uniprot
S26	Phosphorylation		Uniprot
S28	Phosphorylation		Uniprot
K31	Sumoylation		Uniprot
S33	Phosphorylation		Uniprot
T36	Phosphorylation		Uniprot
K38	Methylation		Uniprot
K39	Methylation		Uniprot
K40	Sumoylation		Uniprot
S43	Phosphorylation		Uniprot
S48	Phosphorylation		Uniprot
T50	Phosphorylation		Uniprot
S53	Phosphorylation		Uniprot
K56	Sumoylation		Uniprot
T57	Phosphorylation		Uniprot
T68	Phosphorylation		Uniprot
S72	Phosphorylation		Uniprot
S74	Phosphorylation		Uniprot
K91	Sumoylation		Uniprot
K91	Ubiquitination		Uniprot
T99	Phosphorylation	Q13535 (ATR) , Q13315 (ATM)	Uniprot
K105	Sumoylation		Uniprot
K105	Ubiquitination		Uniprot
T114	Phosphorylation		Uniprot
T122	Phosphorylation	Q13535 (ATR) , Q13315 (ATM)	Uniprot
T125	Phosphorylation		Uniprot
T127	Phosphorylation		Uniprot
K129	Sumoylation		Uniprot
K129	Ubiquitination		Uniprot
S131	Phosphorylation		Uniprot
S142	Phosphorylation		Uniprot
S143	Phosphorylation		Uniprot
S144	Phosphorylation	P33981 (TTK)	Uniprot
K167	Ubiquitination		Uniprot
S168	Phosphorylation		Uniprot
T171	Phosphorylation	P49841 (GSK3B)	Uniprot
S175	Phosphorylation	P24941 (CDK2)	Uniprot
T182	Phosphorylation	O96017 (CHEK2) , O14757 (CHEK1)	Uniprot
K195	Methylation		Uniprot
K195	Sumoylation		Uniprot
Y199	Phosphorylation		Uniprot
T201	Phosphorylation		Uniprot
K225	Ubiquitination		Uniprot
K259	Ubiquitination		Uniprot
S269	Phosphorylation		Uniprot
S282	Phosphorylation		Uniprot
Y296	Phosphorylation		Uniprot
S304	Phosphorylation		Uniprot
K317	Sumoylation		Uniprot
K323	Ubiquitination		Uniprot
T327	Phosphorylation		Uniprot
S328	Phosphorylation		Uniprot
K331	Sumoylation		Uniprot
K331	Ubiquitination		Uniprot
S336	Phosphorylation		Uniprot
T337	Phosphorylation		Uniprot
S338	Phosphorylation	Q8NG66 (NEK11)	Uniprot
K339	Ubiquitination		Uniprot
S343	Phosphorylation		Uniprot
K344	Sumoylation		Uniprot
K344	Ubiquitination		Uniprot
K347	Sumoylation		Uniprot
S349	Phosphorylation		Uniprot
S358	Phosphorylation		Uniprot
T359	Phosphorylation		Uniprot
S367	Phosphorylation		Uniprot
K390	Ubiquitination		Uniprot
K392	Sumoylation		Uniprot
K409	Sumoylation		Uniprot
K409	Ubiquitination		Uniprot
T412	Phosphorylation		Uniprot
K418	Sumoylation		Uniprot
K418	Ubiquitination		Uniprot
S419	Phosphorylation		Uniprot
S422	Phosphorylation		Uniprot
S426	Phosphorylation		Uniprot
S434	Phosphorylation		Uniprot
S443	Phosphorylation		Uniprot
S449	Phosphorylation		Uniprot
S456	Phosphorylation		Uniprot
S462	Phosphorylation		Uniprot
S464	Phosphorylation		Uniprot
T473	Phosphorylation		Uniprot
K476	Ubiquitination		Uniprot
S480	Phosphorylation		Uniprot
K484	Sumoylation		Uniprot
K484	Ubiquitination		Uniprot
K498	Sumoylation		Uniprot
S499	Phosphorylation		Uniprot
S502	Phosphorylation		Uniprot
T508	Phosphorylation		Uniprot
K514	Sumoylation		Uniprot
K514	Ubiquitination		Uniprot
K531	Ubiquitination		Uniprot
K535	Ubiquitination		Uniprot
T537	Phosphorylation		Uniprot
S539	Phosphorylation	O14757 (CHEK1)	Uniprot
S550	Phosphorylation		Uniprot
Y551	Phosphorylation		Uniprot
K578	Ubiquitination		Uniprot
S579	Phosphorylation		Uniprot
K588	Sumoylation		Uniprot
K588	Ubiquitination		Uniprot
K594	Sumoylation		Uniprot
K594	Ubiquitination		Uniprot
S601	Phosphorylation		Uniprot
K604	Ubiquitination		Uniprot
K629	Ubiquitination		Uniprot
S646	Phosphorylation	O14757 (CHEK1) , PR:O14757 (hCHEK1)	Uniprot
K653	Ubiquitination		Uniprot
K657	Acetylation		Uniprot
K661	Acetylation		Uniprot
K662	Acetylation		Uniprot
K662	Sumoylation		Uniprot
K662	Ubiquitination		Uniprot
Y699	Phosphorylation		Uniprot
S714	Phosphorylation	P06493 (CDK1)	Uniprot
Y736	Phosphorylation		Uniprot
K741	Ubiquitination		Uniprot
K755	Ubiquitination		Uniprot
T766	Phosphorylation	P06493 (CDK1)	Uniprot
K769	Ubiquitination		Uniprot
S773	Phosphorylation		Uniprot
K820	Sumoylation		Uniprot
K820	Ubiquitination		Uniprot
T830	Phosphorylation		Uniprot
T832	Phosphorylation		Uniprot
K839	Sumoylation		Uniprot
K839	Ubiquitination		Uniprot
K863	Acetylation		Uniprot
K863	Ubiquitination		Uniprot
K873	Sumoylation		Uniprot
K873	Ubiquitination		Uniprot
T903	Phosphorylation		Uniprot
T907	Phosphorylation		Uniprot
K1057	Ubiquitination		Uniprot
K1082	Sumoylation		Uniprot
K1082	Ubiquitination		Uniprot
S1083	Phosphorylation		Uniprot
K1125	Sumoylation		Uniprot
K1125	Ubiquitination		Uniprot
K1133	Ubiquitination		Uniprot
S1197	Phosphorylation		Uniprot
K1199	Sumoylation		Uniprot
K1199	Ubiquitination		Uniprot
K1207	Ubiquitination		Uniprot
K1217	Ubiquitination		Uniprot
K1273	Sumoylation		Uniprot
K1273	Ubiquitination		Uniprot
S1280	Phosphorylation		Uniprot
S1290	Phosphorylation	P33981 (TTK)	Uniprot
S1295	Phosphorylation		Uniprot
S1296	Phosphorylation	P33981 (TTK)	Uniprot
S1303	Phosphorylation		Uniprot
S1304	Phosphorylation		Uniprot
S1310	Phosphorylation		Uniprot
K1329	Ubiquitination		Uniprot
K1372	Sumoylation		Uniprot
K1372	Ubiquitination		Uniprot
S1375	Phosphorylation		Uniprot
S1383	Phosphorylation		Uniprot
K1411	Acetylation		Uniprot
S1417	Phosphorylation		Uniprot

Research Backgrounds

P54132_BLM_HUMAN

Function:

ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. Participates in DNA replication and repair. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution. Binds single-stranded DNA (ssDNA), forked duplex DNA and DNA Holliday junction.

PTMs:

Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.

Subcellular Location:

Nucleus.
Note: Together with SPIDR, is redistributed in discrete nuclear DNA damage-induced foci following hydroxyurea (HU) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a RMI complex- and SPIDR-dependent manner.

Subunit Structure:

Monomer. Homodimer (via N-terminus). Homotetramer (via N-terminus); dimer of dimers. Homohexamer (via N-terminus). Self-association negatively regulates DNA unwinding amplitude and rate. Oligomeric complexes dissociate into monomer in presence of ATP. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ubiquitinated FANCD2. Interacts with RMI complex. Interacts directly with RMI1 (via N-terminal region) component of RMI complex. Interacts with SUPV3L1. Found in a complex, at least composed of BLM, RAD51 and SPIDR; the complex formation is mediated by SPIDR. Interacts with TOP3A (via N-terminal region). Interacts with SPIDR (via C-terminal region); the interaction is direct and required to target BLM to sites of DNA damage.

Family&Domains:

The N-terminal region mediates dimerization and homooligomerization (PubMed:28228481). Both the helicase ATP-binding domain and the helicase C-terminal domain form intramolecular interactions with the HRDC domain in a ATP-dependent manner (PubMed:25901030). The HRDC domain is required for single-stranded DNA (ssDNA) and DNA Holliday junction binding (PubMed:20639533).

Belongs to the helicase family. RecQ subfamily.

Research Fields

· Genetic Information Processing > Replication and repair > Homologous recombination.

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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Phospho-Blooms Syndrome Protein Blm (Thr99) Antibody - #AF3949