Phospho-STK39 (Ser309) Antibody - #AF3941
| Product: | Phospho-STK39 (Ser309) Antibody |
| Catalog: | AF3941 |
| Description: | Rabbit polyclonal antibody to Phospho-STK39 (Ser309) |
| Application: | ELISA(peptide) |
| Reactivity: | Human |
| Mol.Wt.: | 59kD(Calculated). |
| Uniprot: | Q9UEW8 |
| RRID: | AB_2847664 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF3941, RRID:AB_2847664.
Fold/Unfold
DCHT; DKFZp686K05124; OTTHUMP00000165175; PASK; Proline alanine rich STE20 related kinase; Serine threonine kinase 39 (STE20/SPS1 homolog yeast); Serine threonine kinase 39; Serine/threonine protein kinase 39; Serine/threonine-protein kinase 39; Small intestine SPAK like kinase; SPAK; Ste 20 related kinase; Ste-20-related kinase; Ste20 like protein kinase; STE20/SPS1 homolog; STE20/SPS1 related proline alanine rich protein kinase; STE20/SPS1-related proline-alanine-rich protein kinase; STK 39; Stk39; STK39_HUMAN;
Immunogens
A synthesized peptide derived from human STK39 around the phosphorylation site of Ser309.
Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis.
- Q9UEW8 STK39_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEPSGSPVHVQLPQQAAPVTAAAAAAPAAATAAPAPAAPAAPAPAPAPAAQAVGWPICRDAYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAFSQEKSRRVKEENPEIAVSASTIPEQIQSLSVHDSQGPPNANEDYREASSCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGSEIPDEVKLIGFAQLSVS
PTMs - Q9UEW8 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y63 | Phosphorylation | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| Y182 | Phosphorylation | Uniprot | |
| T219 | Phosphorylation | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| T231 | Phosphorylation | Q9UEW8 (STK39) , Q96J92 (WNK4) , Q9H4A3 (WNK1) | Uniprot |
| T235 | Phosphorylation | Q9UEW8 (STK39) | Uniprot |
| Y273 | Phosphorylation | Uniprot | |
| Y276 | Phosphorylation | Uniprot | |
| Y306 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Q04759 (PRKCQ) | Uniprot |
| K312 | Methylation | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| K320 | Methylation | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| S323 | Phosphorylation | Q04759 (PRKCQ) | Uniprot |
| K324 | Ubiquitination | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| K349 | Acetylation | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| T352 | Phosphorylation | Uniprot | |
| T354 | Phosphorylation | Q9Y3S1 (WNK2) | Uniprot |
| S370 | Phosphorylation | Uniprot | |
| S371 | Phosphorylation | Q9H4A3 (WNK1) , Q96J92 (WNK4) , Q9Y3S1 (WNK2) | Uniprot |
| S385 | Phosphorylation | Uniprot | |
| S401 | Phosphorylation | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| S430 | Phosphorylation | Uniprot | |
| Y444 | Phosphorylation | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| R469 | Methylation | Uniprot | |
| K512 | Acetylation | Uniprot | |
| K512 | Ubiquitination | Uniprot | |
| K515 | Ubiquitination | Uniprot | |
| K520 | Ubiquitination | Uniprot |
PTMs - Q9UEW8 As Enzyme
| Substrate | Site | Source |
|---|---|---|
| P55011 (SLC12A2) | T203 | Uniprot |
| P55011 (SLC12A2) | T207 | Uniprot |
| P55011 (SLC12A2) | T212 | Uniprot |
| P55017 (SLC12A3) | T46 | Uniprot |
| P55017 (SLC12A3) | T55 | Uniprot |
| P55017 (SLC12A3) | T60 | Uniprot |
| P55017 (SLC12A3) | S91 | Uniprot |
| Q13621 (SLC12A1) | T95 | Uniprot |
| Q13621 (SLC12A1) | T100 | Uniprot |
| Q13621 (SLC12A1) | T105 | Uniprot |
| Q9H2X9 (SLC12A5) | T6 | Uniprot |
| Q9H2X9 (SLC12A5) | T1032 | Uniprot |
| Q9UEW8 (STK39) | T231 | Uniprot |
| Q9UEW8 (STK39) | T235 | Uniprot |
| Q9UHW9 (SLC12A6) | S96 | Uniprot |
| Q9UHW9 (SLC12A6) | T160 | Uniprot |
| Q9UHW9 (SLC12A6) | T1048 | Uniprot |
| Q9UP95 (SLC12A4) | T5 | Uniprot |
| Q9UP95 (SLC12A4) | T983 | Uniprot |
| Q9Y666 (SLC12A7) | T973 | Uniprot |
| Q9Y666 (SLC12A7) | T980 | Uniprot |
| Q9Y6R1 (SLC4A4) | T49 | Uniprot |
| Q9Y6R1 (SLC4A4) | S65 | Uniprot |
Research Backgrounds
May act as a mediator of stress-activated signals. Mediates the inhibition of SLC4A4, SLC26A6 as well as CFTR activities by the WNK scaffolds, probably through phosphorylation. Phosphorylates RELT.
Phosphorylated at Ser-309 by PRKCQ. Autophosphorylation at Thr-231 positively regulates its activity (By similarity).
Cytoplasm. Nucleus.
Note: Nucleus when caspase-cleaved.
Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis.
The phosphorylated form forms a complex with WNK2 (By similarity). Interacts with RELT (By similarity). Interacts with SORL1 (via cytosolic C-terminus).
PAPA box (proline-alanine repeats) may target the kinase to a specific subcellular location by facilitating interaction with intracellular proteins such as actin or actin-like proteins.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
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