Product: Phospho-STK39 (Ser309) Antibody
Catalog: AF3941
Description: Rabbit polyclonal antibody to Phospho-STK39 (Ser309)
Application: ELISA(peptide)
Reactivity: Human
Mol.Wt.: 59kD(Calculated).
Uniprot: Q9UEW8
RRID: AB_2847664

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Product Info

Source:
Rabbit
Application:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
Phospho-STK39 (Ser309) Antibody detects endogenous levels of STK39 only when phosphorylated at Ser309.
RRID:
AB_2847664
Cite Format: Affinity Biosciences Cat# AF3941, RRID:AB_2847664.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DCHT; DKFZp686K05124; OTTHUMP00000165175; PASK; Proline alanine rich STE20 related kinase; Serine threonine kinase 39 (STE20/SPS1 homolog yeast); Serine threonine kinase 39; Serine/threonine protein kinase 39; Serine/threonine-protein kinase 39; Small intestine SPAK like kinase; SPAK; Ste 20 related kinase; Ste-20-related kinase; Ste20 like protein kinase; STE20/SPS1 homolog; STE20/SPS1 related proline alanine rich protein kinase; STE20/SPS1-related proline-alanine-rich protein kinase; STK 39; Stk39; STK39_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human STK39 around the phosphorylation site of Ser309.

Uniprot:
Gene(ID):
Expression:
Q9UEW8 STK39_HUMAN:

Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis.

Sequence:
MAEPSGSPVHVQLPQQAAPVTAAAAAAPAAATAAPAPAAPAAPAPAPAPAAQAVGWPICRDAYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAFSQEKSRRVKEENPEIAVSASTIPEQIQSLSVHDSQGPPNANEDYREASSCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGSEIPDEVKLIGFAQLSVS

PTMs - Q9UEW8 As Substrate

Site PTM Type Enzyme
Y63 Phosphorylation
K83 Ubiquitination
K98 Ubiquitination
K160 Ubiquitination
Y182 Phosphorylation
T219 Phosphorylation
T224 Phosphorylation
K230 Ubiquitination
T231 Phosphorylation Q9UEW8 (STK39) , Q96J92 (WNK4) , Q9H4A3 (WNK1)
T235 Phosphorylation Q9UEW8 (STK39)
Y273 Phosphorylation
Y276 Phosphorylation
Y306 Phosphorylation
S309 Phosphorylation Q04759 (PRKCQ)
K312 Methylation
K312 Ubiquitination
K320 Methylation
K320 Ubiquitination
S323 Phosphorylation Q04759 (PRKCQ)
K324 Ubiquitination
K333 Ubiquitination
K341 Ubiquitination
K349 Acetylation
K349 Ubiquitination
T352 Phosphorylation
T354 Phosphorylation Q9Y3S1 (WNK2)
S370 Phosphorylation
S371 Phosphorylation Q9H4A3 (WNK1) , Q96J92 (WNK4) , Q9Y3S1 (WNK2)
S385 Phosphorylation
S401 Phosphorylation
K404 Ubiquitination
S430 Phosphorylation
Y444 Phosphorylation
S448 Phosphorylation
R469 Methylation
K512 Acetylation
K512 Ubiquitination
K515 Ubiquitination
K520 Ubiquitination

PTMs - Q9UEW8 As Enzyme

Substrate Site Source
P55011 (SLC12A2) T203 Uniprot
P55011 (SLC12A2) T207 Uniprot
P55011 (SLC12A2) T212 Uniprot
P55017 (SLC12A3) T46 Uniprot
P55017 (SLC12A3) T55 Uniprot
P55017 (SLC12A3) T60 Uniprot
P55017 (SLC12A3) S91 Uniprot
Q13621 (SLC12A1) T95 Uniprot
Q13621 (SLC12A1) T100 Uniprot
Q13621 (SLC12A1) T105 Uniprot
Q9H2X9 (SLC12A5) T6 Uniprot
Q9H2X9 (SLC12A5) T1032 Uniprot
Q9UEW8 (STK39) T231 Uniprot
Q9UEW8 (STK39) T235 Uniprot
Q9UHW9 (SLC12A6) S96 Uniprot
Q9UHW9 (SLC12A6) T160 Uniprot
Q9UHW9 (SLC12A6) T1048 Uniprot
Q9UP95 (SLC12A4) T5 Uniprot
Q9UP95 (SLC12A4) T983 Uniprot
Q9Y666 (SLC12A7) T973 Uniprot
Q9Y666 (SLC12A7) T980 Uniprot
Q9Y6R1 (SLC4A4) T49 Uniprot
Q9Y6R1 (SLC4A4) S65 Uniprot

Research Backgrounds

Function:

May act as a mediator of stress-activated signals. Mediates the inhibition of SLC4A4, SLC26A6 as well as CFTR activities by the WNK scaffolds, probably through phosphorylation. Phosphorylates RELT.

PTMs:

Phosphorylated at Ser-309 by PRKCQ. Autophosphorylation at Thr-231 positively regulates its activity (By similarity).

Subcellular Location:

Cytoplasm. Nucleus.
Note: Nucleus when caspase-cleaved.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis.

Subunit Structure:

The phosphorylated form forms a complex with WNK2 (By similarity). Interacts with RELT (By similarity). Interacts with SORL1 (via cytosolic C-terminus).

Family&Domains:

PAPA box (proline-alanine repeats) may target the kinase to a specific subcellular location by facilitating interaction with intracellular proteins such as actin or actin-like proteins.

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Restrictive clause

 

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For Research Use Only.
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