Phospho-MKK3 (Thr222) Antibody - #AF3940
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| Product: | Phospho-MKK3 (Thr222) Antibody |
| Catalog: | AF3940 |
| Description: | Rabbit polyclonal antibody to Phospho-MKK3 (Thr222) |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Mol.Wt.: | 39kD(Calculated). |
| Uniprot: | P46734 |
| RRID: | AB_2847663 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF3940, RRID:AB_2847663.
Fold/Unfold
AW212142; dual specificity mitogen activated protein kinase kinase 3; Dual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; map2k3; MAPK ERK kinase 3; MAPK/ERK kinase 3; MAPKK 3; MAPKK3; MEK 3; MEK3; Mitogen activated protein kinase kinase 3; MKK 3; MKK3; mMKK3b; MP2K3_HUMAN; PRKMK 3; PRKMK3; protein kinase, mitogen-activated, kinase 3; SAPK kinase 2; SAPKK 2; SAPKK2; Stress activated protein kinase kinase 2;
Immunogens
A synthesized peptide derived from human MKK3 around the phosphorylation site of Thr222.
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
- P46734 MP2K3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MESPASSQPASMPQSKGKSKRKKDLRISCMSKPPAPNPTPPRNLDSRTFITIGDRNFEVEADDLVTISELGRGAYGVVEKVRHAQSGTIMAVKRIRATVNSQEQKRLLMDLDINMRTVDCFYTVTFYGALFREGDVWICMELMDTSLDKFYRKVLDKNMTIPEDILGEIAVSIVRALEHLHSKLSVIHRDVKPSNVLINKEGHVKMCDFGISGYLVDSVAKTMDAGCKPYMAPERINPELNQKGYNVKSDVWSLGITMIEMAILRFPYESWGTPFQQLKQVVEEPSPQLPADRFSPEFVDFTAQCLRKNPAERMSYLELMEHPFFTLHKTKKTDIAAFVKEILGEDS
PTMs - P46734 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S3 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| S31 | Phosphorylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| T39 | Phosphorylation | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| T193 | Phosphorylation | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| Y214 | Phosphorylation | Uniprot | |
| S218 | Phosphorylation | Q99759 (MAP3K3) , P41279 (MAP3K8) , Q9UL54 (TAOK2) , Q16584 (MAP3K11) , Q7L7X3 (TAOK1) , Q99683 (MAP3K5) , P46734 (MAP2K3) | Uniprot |
| T222 | Phosphorylation | Q16584 (MAP3K11) , P46734 (MAP2K3) | Uniprot |
| Y230 | Phosphorylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| Y245 | Phosphorylation | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| K279 | Sumoylation | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K332 | Ubiquitination | Uniprot | |
| K340 | Ubiquitination | Uniprot |
PTMs - P46734 As Enzyme
Research Backgrounds
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity. Phosphorylated by TAOK2.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, ZAK and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and ZAK. Binds to DYRK1B/MIRK and increases its kinase activity. Part of a complex with MAP3K3, RAC1 and CCM2 (By similarity). Interacts with ARRB1. Interacts with Yersinia yopJ.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Research Fields
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)
· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)
References
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