AKAP10 Antibody - #DF3024
| Product: | AKAP10 Antibody |
| Catalog: | DF3024 |
| Description: | Rabbit polyclonal antibody to AKAP10 |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 73 KD; 74kD(Calculated). |
| Uniprot: | O43572 |
| RRID: | AB_2835055 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3024, RRID:AB_2835055.
Fold/Unfold
1500031L16Rik; A kinase (PRKA) anchor protein 10; A kinase anchor protein 10; A kinase anchor protein 10 mitochondrial; A-kinase anchor protein 10; AKA10_HUMAN; akap 10; AKAP-10; AKAP10; B130049N18Rik; D AKAP 2; D-AKAP-2; dakap 2; dakap2; Dual specific A kinase anchoring protein 2; Dual specificity A kinase-anchoring protein 2; mgc9410; MGC9414|; Mitochondrial A kinase PPKA anchor protein 10; mitochondrial; prka 10; PRKA10; Protein A kinase anchoring protein 10; Protein A kinase anchoring protein 10 mitochondrial precursor; Protein kinase A anchoring protein 10; Protein kinase A-anchoring protein 10;
Immunogens
- O43572 AKA10_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRGAGPSPRQSPRTLRPDPGPAMSFFRRKVKGKEQEKTSDVKSIKASISVHSPQKSTKNHALLEAAGPSHVAINAISANMDSFSSSRTATLKKQPSHMEAAHFGDLGRSCLDYQTQETKSSLSKTLEQVLHDTIVLPYFIQFMELRRMEHLVKFWLEAESFHSTTWSRIRAHSLNTVKQSSLAEPVSPSKKHETTASFLTDSLDKRLEDSGSAQLFMTHSEGIDLNNRTNSTQNHLLLSQECDSAHSLRLEMARAGTHQVSMETQESSSTLTVASRNSPASPLKELSGKLMKSIEQDAVNTFTKYISPDAAKPIPITEAMRNDIIARICGEDGQVDPNCFVLAQSIVFSAMEQEHFSEFLRSHHFCKYQIEVLTSGTVYLADILFCESALFYFSEYMEKEDAVNILQFWLAADNFQSQLAAKKGQYDGQEAQNDAMILYDKYFSLQATHPLGFDDVVRLEIESNICREGGPLPNCFTTPLRQAWTTMEKVFLPGFLSSNLYYKYLNDLIHSVRGDEFLGGNVSLTAPGSVGPPDESHPGSSDSSASQSSVKKASIKILKNFDEAIIVDAASLDPESLYQRTYAGKMTFGRVSDLGQFIRESEPEPDVRKSKGSMFSQAMKKWVQGNTDEAQEELAWKIAKMIVSDIMQQAQYDQPLEKSTKL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O43572 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R2 | Methylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| R9 | Methylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| K55 | Methylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| T90 | Phosphorylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| Y113 | Phosphorylation | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| T229 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| T272 | Phosphorylation | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| S281 | Phosphorylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| K423 | Ubiquitination | Uniprot | |
| Y442 | Phosphorylation | Uniprot | |
| T448 | Phosphorylation | Uniprot | |
| K559 | Ubiquitination | Uniprot | |
| K585 | Ubiquitination | Uniprot | |
| K611 | Ubiquitination | Uniprot | |
| K621 | Ubiquitination | Uniprot | |
| K637 | Ubiquitination | Uniprot | |
| K658 | Ubiquitination | Uniprot |
Research Backgrounds
Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A and anchors them to the mitochondria or the plasma membrane. Although the physiological relevance between PKA and AKAPS with mitochondria is not fully understood, one idea is that BAD, a proapoptotic member, is phosphorylated and inactivated by mitochondria-anchored PKA. It cannot be excluded too that it may facilitate PKA as well as G protein signal transduction, by acting as an adapter for assembling multiprotein complexes. With its RGS domain, it could lead to the interaction to G-alpha proteins, providing a link between the signaling machinery and the downstream kinase (By similarity).
Mitochondrion. Membrane. Cytoplasm.
Note: Predominantly mitochondrial but also membrane associated and cytoplasmic.
RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
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