Phospho-WWOX (Tyr33) Antibody | Affinity Biosciences

Product:	Phospho-WWOX (Tyr33) Antibody
Catalog:	AF3928
Description:	Rabbit polyclonal antibody to Phospho-WWOX (Tyr33)
Application:	ELISA(peptide)
Reactivity:	Human, Mouse
Mol.Wt.:	47kD(Calculated).
Uniprot:	Q9NZC7
RRID:	AB_2847651

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

Phospho-WWOX (Tyr33) Antibody detects endogenous levels of WWOX only when phosphorylated at Tyr33.

RRID:

AB_2847651
Cite Format: Affinity Biosciences Cat# AF3928, RRID:AB_2847651.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

A synthesized peptide derived from human WWOX around the phosphorylation site of Tyr33.

Uniprot:

Q9NZC7(WWOX_HUMAN) >>Visit HPA database.

Expression:

Q9NZC7 WWOX_HUMAN:

Widely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines.

Sequence:

Q9NZC7 WWOX_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAALRYAGLDDTDSEDELPPGWEERTTKDGWVYYANHTEEKTQWEHPKTGKRKRVAGDLPYGWEQETDENGQVFFVDHINKRTTYLDPRLAFTVDDNPTKPTTRQRYDGSTTAMEILQGRDFTGKVVVVTGANSGIGFETAKSFALHGAHVILACRNMARASEAVSRILEEWHKAKVEAMTLDLALLRSVQHFAEAFKAKNVPLHVLVCNAATFALPWSLTKDGLETTFQVNHLGHFYLVQLLQDVLCRSAPARVIVVSSESHRFTDINDSLGKLDFSRLSPTKNDYWAMLAYNRSKLCNILFSNELHRRLSPRGVTSNAVHPGNMMYSNIHRSWWVYTLLFTLARPFTKSMQQGAATTVYCAAVPELEGLGGMYFNNCCRCMPSPEAQSEETARTLWALSERLIQERLGSQSG

PTMs - Q9NZC7 As Substrate

Q9NZC7

Site	PTM Type	Enzyme	Source
Y6	Phosphorylation		Uniprot
T12	Phosphorylation		Uniprot
S14	Phosphorylation		Uniprot
K28	Ubiquitination		Uniprot
Y33	Phosphorylation	P12931 (SRC)	Uniprot
K48	Ubiquitination		Uniprot
Y61	Phosphorylation		Uniprot
K100	Ubiquitination		Uniprot
T111	Phosphorylation		Uniprot
S134	Phosphorylation		Uniprot
S162	Phosphorylation		Uniprot
K274	Ubiquitination		Uniprot
Y287	Phosphorylation	Q07912 (TNK2)	Uniprot
S312	Phosphorylation		Uniprot

Research Backgrounds

Q9NZC7_WWOX_HUMAN

Function:

Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.

PTMs:

Phosphorylated upon genotoxic stress. Phosphorylation of Tyr-33 regulates interaction with TP53, TP73 and MAPK8. May also regulate proapoptotic activity. Phosphorylation by TNK2 is associated with polyubiquitination and degradation.

Ubiquitinated when phosphorylated by TNK2, leading to its degradation.

Subcellular Location:

Cytoplasm. Nucleus. Mitochondrion. Golgi apparatus.
Note: Partially localizes to the mitochondria (PubMed:14695174). Translocates to the nucleus upon genotoxic stress or TNF stimulation (By similarity). Translocates to the nucleus in response to TGFB1 (PubMed:19366691). Isoform 5 and isoform 6 may localize in the nucleus.

Tissue Specificity:

Widely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines.

Subunit Structure:

Interacts with TP53, p73/TP73 and MAPK8. Interacts with MAPT/TAU, RUNX2 and HYAL2 (By similarity). Forms a ternary complex with TP53 and MDM2. Interacts with ERBB4, LITAF and WBP1. Interacts with DVL1, DVL2 and DVL3. May interact with FAM189B and SCOTIN. Interacts with TNK2. Interacts with TMEM207.

Family&Domains:

The WW 1 domain mediates interaction with TP53, and probably TP73, TFAP2C, LITAF and WBP1.

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Restrictive clause

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Phospho-WWOX (Tyr33) Antibody - #AF3928