Phospho-UBF1 (Ser484) Antibody - #AF3925
(1)
| Product: | Phospho-UBF1 (Ser484) Antibody |
| Catalog: | AF3925 |
| Description: | Rabbit polyclonal antibody to Phospho-UBF1 (Ser484) |
| Application: | IHC |
| Reactivity: | Human, Mouse, Rat |
| Mol.Wt.: | 89kD(Calculated). |
| Uniprot: | P17480 |
| RRID: | AB_2847648 |
Product Info
Source:
Rabbit
Application:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-UBF1 (Ser484) Antibody detects endogenous levels of UBF1 only when phosphorylated at Ser484.
RRID:
AB_2847648
Cite Format: Affinity Biosciences Cat# AF3925, RRID:AB_2847648.
Cite Format: Affinity Biosciences Cat# AF3925, RRID:AB_2847648.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
90 kDa nucleolus organizer region autoantigen; Autoantigen NOR-90; NOR 90; Nucleolar transcription factor 1; UBF 1; UBF; UBF-1; UBF1_HUMAN; Ubtf; Upstream binding factor 1; upstream binding transcription factor, RNA polymerase I; Upstream-binding factor 1;
Immunogens
Immunogen:
A synthesized peptide derived from human UBF1 around the phosphorylation site of Ser484.
Uniprot:
Gene(ID):
Sequence:
- P17480 UBF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNGEADCPTDLEMAAPKGQDRWSQEDMLTLLECMKNNLPSNDSSKFKTTESHMDWEKVAFKDFSGDMCKLKWVEISNEVRKFRTLTELILDAQEHVKNPYKGKKLKKHPDFPKKPLTPYFRFFMEKRAKYAKLHPEMSNLDLTKILSKKYKELPEKKKMKYIQDFQREKQEFERNLARFREDHPDLIQNAKKSDIPEKPKTPQQLWYTHEKKVYLKVRPDATTKEVKDSLGKQWSQLSDKKRLKWIHKALEQRKEYEEIMRDYIQKHPELNISEEGITKSTLTKAERQLKDKFDGRPTKPPPNSYSLYCAELMANMKDVPSTERMVLCSQQWKLLSQKEKDAYHKKCDQKKKDYEVELLRFLESLPEEEQQRVLGEEKMLNINKKQATSPASKKPAQEGGKGGSEKPKRPVSAMFIFSEEKRRQLQEERPELSESELTRLLARMWNDLSEKKKAKYKAREAALKAQSERKPGGEREERGKLPESPKRAEEIWQQSVIGDYLARFKNDRVKALKAMEMTWNNMEKKEKLMWIKKAAEDQKRYERELSEMRAPPAATNSSKKMKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKERMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHLDLWVKSLSPQDRAAYKEYISNKRKSMTKLRGPNPKSSRTTLQSKSESEEDDEEDEDDEDEDEEEEDDENGDSSEDGGDSSESSSEDESEDGDENEEDDEDEDDDEDDDEDEDNESEGSSSSSSSSGDSSDSDSN
PTMs - P17480 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| M1 | Acetylation | Uniprot | |
| T9 | Phosphorylation | Uniprot | |
| K17 | Ubiquitination | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K61 | Acetylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K114 | Acetylation | Uniprot | |
| K114 | Sumoylation | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| T117 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| K126 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| Y150 | Phosphorylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K160 | Sumoylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| T201 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| Y207 | Phosphorylation | Uniprot | |
| T208 | Phosphorylation | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| K232 | Methylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| K254 | Methylation | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K284 | Acetylation | Uniprot | |
| K284 | Sumoylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| Y308 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| K352 | Acetylation | Uniprot | |
| K352 | Ubiquitination | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K385 | Acetylation | Uniprot | |
| T388 | Phosphorylation | Uniprot | |
| S389 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S392 | Phosphorylation | Uniprot | |
| K394 | Acetylation | Uniprot | |
| K401 | Acetylation | Uniprot | |
| K406 | Acetylation | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| K421 | Ubiquitination | Uniprot | |
| R429 | Methylation | Uniprot | |
| S433 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| R443 | Methylation | Uniprot | |
| S449 | Phosphorylation | Uniprot | |
| Y456 | Phosphorylation | Uniprot | |
| K464 | Sumoylation | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| S484 | Phosphorylation | P24941 (CDK2) , P11802 (CDK4) | Uniprot |
| S495 | Phosphorylation | Uniprot | |
| Y500 | Phosphorylation | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| K559 | Acetylation | Uniprot | |
| S584 | Phosphorylation | Uniprot | |
| K594 | Ubiquitination | Uniprot | |
| S602 | Phosphorylation | Uniprot | |
| S610 | Phosphorylation | Uniprot | |
| K624 | Ubiquitination | Uniprot | |
| S638 | Phosphorylation | Uniprot | |
| R642 | Methylation | Uniprot | |
| K646 | Ubiquitination | Uniprot | |
| Y648 | Phosphorylation | Uniprot | |
| S655 | Phosphorylation | Uniprot | |
| S666 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
PTMs:
Phosphorylated and activated by PIK3CA.
Subcellular Location:
Nucleus>Nucleolus.
Subunit Structure:
Homodimer (By similarity). Interacts with TBP. Interacts with TAF1A. Interacts with RASL11A (By similarity). Binds to IRS1 and PIK3CA (By similarity). Interacts with DHX33. Interacts with PHF6. Interacts with CEBPA (isoform 1 and isoform 4). Interacts with DDX11. Interacts with NOP53.
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For Research Use Only.
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