Phospho-CAD (Thr456) Antibody | Affinity Biosciences

Product:	Phospho-CAD (Thr456) Antibody
Catalog:	AF3916
Description:	Rabbit polyclonal antibody to Phospho-CAD (Thr456)
Application:	ELISA(peptide)
Reactivity:	Human
Mol.Wt.:	243kD(Calculated).
Uniprot:	P27708
RRID:	AB_2847639

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

Phospho-CAD (Thr456) Antibody detects endogenous levels of CAD only when phosphorylated at Thr456.

RRID:

AB_2847639
Cite Format: Affinity Biosciences Cat# AF3916, RRID:AB_2847639.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Aspartate carbamoyltransferase; Aspartate transcarbamylase; CAD protein; CAD trifunctional protein; Carbamoyl phosphate synthetase 2; Carbamoyl-phosphate synthetase 2 aspartate transcarbamylase and dihydroorotase; CPSase ATCase DHOase; Dihydroorotase; Glutamine dependent carbamoyl phosphate synthase; Multifunctional protein CAD;

Immunogens

Immunogen:

A synthesized peptide derived from human CAD around the phosphorylation site of Thr456.

Uniprot:

P27708(PYR1_HUMAN) >>Visit HPA database.

Gene(ID):

CAD(790)

Sequence:

P27708 PYR1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAALVLEDGSVLRGQPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPPDEMDEFGLCKWFESSGIHVAALVVGECCPTPSHWSATRTLHEWLQQHGIPGLQGVDTRELTKKLREQGSLLGKLVQNGTEPSSLPFLDPNARPLVPEVSIKTPRVFNTGGAPRILALDCGLKYNQIRCLCQRGAEVTVVPWDHALDSQEYEGLFLSNGPGDPASYPSVVSTLSRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLVGSGRCFLTSQNHGFAVETDSLPADWAPLFTNANDGSNEGIVHNSLPFFSVQFHPEHQAGPSDMELLFDIFLETVKEATAGNPGGQTVRERLTERLCPPGIPTPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITPHYVTQVIRNERPDGVLLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASNREELSALVAPAFAHTSQVLVDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGESIVVAPSQTLNDREYQLLRQTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPELRNSVTGGTAAFEPSVDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGRSFEEAFQKALRMVDENCVGFDHTVKPVSDMELETPTDKRIFVVAAALWAGYSVDRLYELTRIDRWFLHRMKRIIAHAQLLEQHRGQPLPPDLLQQAKCLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYWGTTHDLTFRTPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCQTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVASDGVVAAIAISEHVENAGVHSGDATLVTPPQDITAKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRVIMGEEVEPVGLMTGSGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPKKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILEQLAEKNFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGITMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFALFLGASSENAGTLGTVAGSAAGLKLYLNETFSELRLDSVVQWMEHFETWPSHLPIVAHAEQQTVAAVLMVAQLTQRSVHICHVARKEEILLIKAAKARGLPVTCEVAPHHLFLSHDDLERLGPGKGEVRPELGSRQDVEALWENMAVIDCFASDHAPHTLEEKCGSRPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPPQEDTYVEVDLEHEWTIPSHMPFSKAHWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGAVPQLPPSAPATSEMTTTPERPRRGIPGLPDGRFHLPPRIHRASDPGLPAEEPKEKSSRKVAEPELMGTPDGTCYPPPPVPRQASPQNLGTPGLLHPQTSPLLHSLVGQHILSVQQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPTVRAFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSTQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF

PTMs - P27708 As Substrate

P27708

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
S10	Phosphorylation		Uniprot
K127	Ubiquitination		Uniprot
S133	Phosphorylation		Uniprot
K137	Ubiquitination		Uniprot
K165	Ubiquitination		Uniprot
T166	Phosphorylation		Uniprot
T172	Phosphorylation		Uniprot
R177	Methylation		Uniprot
K186	Acetylation		Uniprot
K186	Methylation		Uniprot
K186	Ubiquitination		Uniprot
Y267	Phosphorylation		Uniprot
T375	Phosphorylation		Uniprot
K424	Ubiquitination		Uniprot
T456	Phosphorylation	P28482 (MAPK1) , P27361 (MAPK3)	Uniprot
T475	Phosphorylation		Uniprot
K490	Ubiquitination		Uniprot
T504	Phosphorylation		Uniprot
T585	Phosphorylation		Uniprot
S586	Phosphorylation		Uniprot
K592	Ubiquitination		Uniprot
K598	Ubiquitination		Uniprot
Y602	Phosphorylation		Uniprot
K696	Ubiquitination		Uniprot
Y735	Phosphorylation		Uniprot
K739	Ubiquitination		Uniprot
K747	Acetylation		Uniprot
K747	Ubiquitination		Uniprot
K754	Ubiquitination		Uniprot
K760	Ubiquitination		Uniprot
S761	Phosphorylation		Uniprot
K778	Ubiquitination		Uniprot
K795	Ubiquitination		Uniprot
K808	Ubiquitination		Uniprot
K867	Ubiquitination		Uniprot
K874	Ubiquitination		Uniprot
T883	Phosphorylation		Uniprot
T1037	Phosphorylation	P27708 (CAD)	Uniprot
S1038	Phosphorylation		Uniprot
S1044	Phosphorylation		Uniprot
S1061	Phosphorylation		Uniprot
S1069	Phosphorylation		Uniprot
S1073	Phosphorylation		Uniprot
S1112	Phosphorylation		Uniprot
K1119	Ubiquitination		Uniprot
K1127	Ubiquitination		Uniprot
K1177	Ubiquitination		Uniprot
K1206	Ubiquitination		Uniprot
K1211	Ubiquitination		Uniprot
S1220	Phosphorylation		Uniprot
S1222	Phosphorylation		Uniprot
K1228	Ubiquitination		Uniprot
K1262	Ubiquitination		Uniprot
S1267	Phosphorylation		Uniprot
K1301	Methylation		Uniprot
K1309	Ubiquitination		Uniprot
K1313	Ubiquitination		Uniprot
K1323	Ubiquitination		Uniprot
K1325	Ubiquitination		Uniprot
K1387	Ubiquitination		Uniprot
S1406	Phosphorylation	P17612 (PRKACA)	Uniprot
S1407	Phosphorylation		Uniprot
K1411	Acetylation		Uniprot
K1411	Ubiquitination		Uniprot
S1423	Phosphorylation		Uniprot
K1431	Ubiquitination		Uniprot
K1434	Ubiquitination		Uniprot
K1450	Sumoylation		Uniprot
K1450	Ubiquitination		Uniprot
K1460	Ubiquitination		Uniprot
K1521	Ubiquitination		Uniprot
K1618	Ubiquitination		Uniprot
K1625	Ubiquitination		Uniprot
S1646	Phosphorylation		Uniprot
K1657	Ubiquitination		Uniprot
S1698	Phosphorylation		Uniprot
S1724	Phosphorylation		Uniprot
K1778	Ubiquitination		Uniprot
Y1794	Phosphorylation		Uniprot
Y1805	Phosphorylation		Uniprot
S1823	Phosphorylation		Uniprot
T1827	Phosphorylation		Uniprot
S1828	Phosphorylation		Uniprot
T1831	Phosphorylation		Uniprot
T1832	Phosphorylation		Uniprot
T1833	Phosphorylation		Uniprot
R1839	Methylation		Uniprot
H1856	Phosphorylation		Uniprot
S1859	Phosphorylation	P23443 (RPS6KB1) , P17612 (PRKACA)	Uniprot
S1873	Phosphorylation		Uniprot
T1884	Phosphorylation		Uniprot
T1888	Phosphorylation		Uniprot
C1889	S-Nitrosylation		Uniprot
Y1890	Phosphorylation		Uniprot
S1900	Phosphorylation		Uniprot
T1906	Phosphorylation		Uniprot
T1914	Phosphorylation		Uniprot
S1920	Phosphorylation		Uniprot
S1938	Phosphorylation		Uniprot
K1953	Ubiquitination		Uniprot
K1961	Ubiquitination		Uniprot
K2036	Ubiquitination		Uniprot
Y2103	Phosphorylation		Uniprot
K2125	Ubiquitination		Uniprot
S2154	Phosphorylation		Uniprot
T2155	Phosphorylation		Uniprot
Y2158	Phosphorylation		Uniprot
K2178	Ubiquitination		Uniprot
Y2203	Phosphorylation		Uniprot
Y2212	Phosphorylation		Uniprot
T2220	Phosphorylation		Uniprot

PTMs - P27708 As Enzyme

P27708

Substrate	Site	Source
P27708 (CAD)	T1037	Uniprot

Research Backgrounds

P27708_PYR1_HUMAN

Function:

This protein is a 'fusion' protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).

PTMs:

Activated by MAP kinase (Erk1/2) phosphorylation just prior to the S phase of the cell cycle, when the demand for pyrimidine nucleotides is greatest, and down-regulated as the cells emerge from S phase by protein kinase A (PKA) phosphorylation. Phosphorylation at Ser-1859 by RPS6KB1 downstream of MTOR promotes oligomerization and stimulates dihydroorotase activity. Phosphorylation at Ser-1406 reduces sensitivity to feedback inhibition by UTP.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Cytosolic and unphosphorylated in resting cells, translocates to the nucleus in response to EGF stimulation, nuclear import promotes optimal cell growth.

Subunit Structure:

Homohexamer. Interacts with CIPC.

Family&Domains:

In the central section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily.

Research Fields

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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Phospho-CAD (Thr456) Antibody - #AF3916