Product: Phospho-Hck (Tyr522) Antibody
Catalog: AF3915
Description: Rabbit polyclonal antibody to Phospho-Hck (Tyr522)
Application: ELISA(peptide)
Reactivity: Human, Mouse, Rat
Mol.Wt.: 60kD(Calculated).
Uniprot: P08631
RRID: AB_2847638

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 100ul $350 In stock
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Product Info

Source:
Rabbit
Application:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-Hck (Tyr522) Antibody detects endogenous levels of Hck only when phosphorylated at Tyr522.
RRID:
AB_2847638
Cite Format: Affinity Biosciences Cat# AF3915, RRID:AB_2847638.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Bmk; Hck 1; Hck; HCK_HUMAN; Hemopoietic cell kinase; JTK9; p59-HCK/p60-HCK; p59Hck; p59HCK/p60HCK; p61Hck; Tyrosine protein kinase HCK; Tyrosine-protein kinase HCK;

Immunogens

Immunogen:

A synthesized peptide derived from human Hck around the phosphorylation site of Tyr522.

Uniprot:
Gene(ID):
Expression:
P08631 HCK_HUMAN:

Detected in monocytes and neutrophils (at protein level). Expressed predominantly in cells of the myeloid and B-lymphoid lineages. Highly expressed in granulocytes. Detected in tonsil.

Sequence:
MGGRSSCEDPGCPRDEERAPRMGCMKSKFLQVGGNTFSKTETSASPHCPVYVPDPTSTIKPGPNSHNSNTPGIREAGSEDIIVVALYDYEAIHHEDLSFQKGDQMVVLEESGEWWKARSLATRKEGYIPSNYVARVDSLETEEWFFKGISRKDAERQLLAPGNMLGSFMIRDSETTKGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRSTFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLAEANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPEELYNIMMRCWKNRPEERPTFEYIQSVLDDFYTATESQYQQQP

PTMs - P08631 As Substrate

Site PTM Type Enzyme
G2 Myristoylation
K28 Ubiquitination
T36 Phosphorylation
Y51 Phosphorylation P08631 (HCK)
Y89 Phosphorylation
S111 Phosphorylation
S119 Phosphorylation
K124 Ubiquitination
Y127 Phosphorylation
Y180 Phosphorylation
S181 Phosphorylation
T202 Phosphorylation
Y209 Phosphorylation P08631 (HCK)
K228 Acetylation
S238 Phosphorylation
S261 Phosphorylation
K283 Ubiquitination
K286 Ubiquitination
T311 Phosphorylation
Y330 Phosphorylation
S340 Phosphorylation
Y411 Phosphorylation P08631 (HCK)
T412 Phosphorylation
K418 Ubiquitination
S442 Phosphorylation
S462 Phosphorylation
S520 Phosphorylation
Y522 Phosphorylation P08631 (HCK) , P42679 (MATK)

PTMs - P08631 As Enzyme

Substrate Site Source
O15350 (TP73) Y28 Uniprot
O60496 (DOK2) Y271 Uniprot
O60496 (DOK2) Y299 Uniprot
O60496 (DOK2) Y345 Uniprot
P00519 (ABL1) Y70 Uniprot
P00519 (ABL1) Y115 Uniprot
P00519 (ABL1) Y128 Uniprot
P00519 (ABL1) Y139 Uniprot
P00519 (ABL1) Y172 Uniprot
P00519 (ABL1) Y185 Uniprot
P00519 (ABL1) Y215 Uniprot
P00519 (ABL1) Y226 Uniprot
P00519 (ABL1) Y393 Uniprot
P08631 (HCK) Y51 Uniprot
P08631 (HCK) Y209 Uniprot
P08631 (HCK) Y411 Uniprot
P08631 (HCK) Y522 Uniprot
P11274-1 (BCR) Y177 Uniprot
P16885 (PLCG2) Y753 Uniprot
P16885 (PLCG2) Y759 Uniprot
P19174-1 (PLCG1) Y771 Uniprot
P19174-1 (PLCG1) Y783 Uniprot
P19174-1 (PLCG1) Y1253 Uniprot
P22681 (CBL) Y731 Uniprot
P40763-2 (STAT3) Y704 Uniprot
P40763-1 (STAT3) Y705 Uniprot
P42229 (STAT5A) Y694 Uniprot
P42768 (WAS) Y291 Uniprot
P51692 (STAT5B) Y699 Uniprot
Q05655 (PRKCD) Y313 Uniprot
Q13444 (ADAM15) Y715 Uniprot
Q13444 (ADAM15) Y735 Uniprot
Q13905 (RAPGEF1) Y504 Uniprot
Q13905-3 (RAPGEF1) Y522 Uniprot
Q92556-1 (ELMO1) Y18 Uniprot
Q92556-2 (ELMO1) Y31 Uniprot
Q92556-1 (ELMO1) Y216 Uniprot
Q92556-2 (ELMO1) Y240 Uniprot
Q92556-1 (ELMO1) Y395 Uniprot
Q92556 (ELMO1) Y511 Uniprot
Q92556 (ELMO1) Y720 Uniprot
Q99062-1 (CSF3R) Y752 Uniprot
Q99062-3 (CSF3R) Y779 Uniprot
Q99062-1 (CSF3R) Y787 Uniprot
Q99062-3 (CSF3R) Y814 Uniprot
Q9UQC2 (GAB2) Y452 Uniprot

Research Backgrounds

Function:

Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.

PTMs:

Phosphorylated on several tyrosine residues. Autophosphorylated. Becomes rapidly phosphorylated upon activation of the immunoglobulin receptors FCGR1A and FCGR2A. Phosphorylation by the BCR-ABL fusion protein mediates activation of HCK. Phosphorylation at Tyr-411 increases kinase activity. Phosphorylation at Tyr-522 inhibits kinase activity. Kinase activity is not required for phosphorylation at Tyr-522, suggesting that this site is a target of other kinases.

Ubiquitinated by CBL, leading to its degradation via the proteasome.

Isoform 2 palmitoylation at position 2 requires prior myristoylation. Palmitoylation at position 3 is required for caveolar localization of isoform 2.

Subcellular Location:

Lysosome. Membrane>Lipid-anchor. Cell projection>Podosome membrane>Lipid-anchor. Cytoplasm>Cytosol.
Note: Associated with specialized secretory lysosomes called azurophil granules. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated.

Cell membrane>Lipid-anchor. Membrane>Caveola>Lipid-anchor. Cell junction>Focal adhesion. Cytoplasm>Cytoskeleton. Golgi apparatus. Cytoplasmic vesicle. Lysosome. Nucleus.
Note: 20% of this isoform is associated with caveolae. Localization at the cell membrane and at caveolae requires palmitoylation at Cys-3. Colocalizes with the actin cytoskeleton at focal adhesions.

Cytoplasmic vesicle>Secretory vesicle. Cytoplasm>Cytosol.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in monocytes and neutrophils (at protein level). Expressed predominantly in cells of the myeloid and B-lymphoid lineages. Highly expressed in granulocytes. Detected in tonsil.

Subunit Structure:

Interacts (via SH2 domain) with FLT3 (tyrosine phosphorylated). Interacts with VAV1, WAS and RAPGEF1 (By similarity). This interaction stimulates its tyrosine-kinase activity. Interacts with ARRB1 and ARRB2. Interacts with ADAM15. Interacts with FASLG. Interacts with CBL. Interacts with FCGR1A; the interaction may be indirect. Interacts with IL6ST. Interacts (via SH3 domain) with ELMO1. Interacts (via SH3 domain) with TP73. Interacts with YAP1. Interacts with ABL1 and ITGB1, and thereby recruits ABL1 to activated ITGB1. Interacts (via SH3 domain) with WDCP.

(Microbial infection) Interacts (via SH3 domain) with HEV ORF3 protein.

(Microbial infection) Interacts (via SH3 domain) with HIV-1 Nef and Vif.

Family&Domains:

The SH3 domain mediates binding to HIV-1 Nef.

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Research Fields

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

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