Smad5 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Mouse lung, using Smad5 Antibody.

Product:	Smad5 Antibody
Catalog:	AF6899
Description:	Rabbit polyclonal antibody to Smad5
Application:	WB
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	52kD(Calculated).
Uniprot:	Q99717
RRID:	AB_2847622

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Smad5 Antibody detects endogenous levels of total Smad5.

RRID:

AB_2847622
Cite Format: Affinity Biosciences Cat# AF6899, RRID:AB_2847622.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DKFZp781C1895; DKFZp781O1323; Dwfc; hSmad5; JV5 1; JV5-1; MAD homolog 5; MAD, mothers against decapentaplegic homolog 5; MADH 5; MADH5; Mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; Mothers against DPP homolog 5; MusMLP; SMA and MAD related protein 5; SMAD 5; SMAD family member 5; SMAD, mothers against DPP homolog 5; Smad5; SMAD5_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human Smad5.

Uniprot:

Q99717(SMAD5_HUMAN) >>Visit HPA database.

Gene(ID):

SMAD5(4090)

Expression:

Q99717 SMAD5_HUMAN:

Ubiquitous.

Sequence:

Q99717 SMAD5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTSMASLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSSPGQPSKCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLDICEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHNEFNPQHSLLVQFRNLSHNEPHMPQNATFPDSFHQPNNTPFPLSPNSPYPPSPASSTYPNSPASSGPGSPFQLPADTPPPAYMPPDDQMGQDNSQPMDTSNNMIPQIMPSISSRDVQPVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKSRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNFHHGFHPTTVCKIPSSCSLKIFNNQEFAQLLAQSVNHGFEAVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPLNPISSVS

PTMs - Q99717 As Substrate

Q99717

Site	PTM Type	Source
T2	Acetylation	Uniprot
S12	Phosphorylation	Uniprot
K22	Ubiquitination	Uniprot
K33	Ubiquitination	Uniprot
S57	Phosphorylation	Uniprot
S58	Phosphorylation	Uniprot
S63	Phosphorylation	Uniprot
K64	Ubiquitination	Uniprot
S79	Phosphorylation	Uniprot
K82	Ubiquitination	Uniprot
Y89	Phosphorylation	Uniprot
K117	Sumoylation	Uniprot
K119	Sumoylation	Uniprot
K119	Ubiquitination	Uniprot
Y128	Phosphorylation	Uniprot
S133	Phosphorylation	Uniprot
S152	Phosphorylation	Uniprot
S188	Phosphorylation	Uniprot
K306	Ubiquitination	Uniprot
S315	Phosphorylation	Uniprot
K418	Ubiquitination	Uniprot
S462	Phosphorylation	Uniprot
S463	Phosphorylation	Uniprot
S465	Phosphorylation	Uniprot

Research Backgrounds

Q99717_SMAD5_HUMAN

Function:

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

PTMs:

Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.

Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

May form trimers with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit and SMURF1. Interacts with SUV39H1 and SUV39H2. Interacts (via MH2 domain) with LEMD3. Interacts with WWP1. Interacts with TMEM119 (By similarity). Interacts with ZNF8. Interacts with RANBP3L.

Family&Domains:

Belongs to the dwarfin/SMAD family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)

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For Research Use Only.
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Smad5 Antibody - #AF6899