Integrin beta 4 Antibody - #AF6871

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Product: Integrin beta 4 Antibody
Catalog: AF6871
Description: Rabbit polyclonal antibody to Integrin beta 4
Application: WB
Reactivity: Human, Mouse, Rat
Mol.Wt.: 200kD; 202kD(Calculated).
Uniprot: P16144
RRID: AB_2847594

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 100ul $280 In stock
 200ul $350 In stock

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Integrin beta 4 Antibody detects endogenous levels of total Integrin beta 4.
RRID:
AB_2847594
Cite Format: Affinity Biosciences Cat# AF6871, RRID:AB_2847594.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD 104; CD104; CD104 antigen; gp150; Integrin beta 4 subunit; Integrin beta-4; ITB4_HUMAN; ITG B4; ITGB 4; Itgb4;

Immunogens

Immunogen:

A synthesized peptide derived from human Integrin beta 4.

Uniprot:

P16144(ITB4_HUMAN) >>Visit HPA database.

Gene(ID):
ITGB4(3691)
Expression:
P16144 ITB4_HUMAN:

Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.

Sequence:
MAGPRPSPWARLLLAALISVSLSGTLANRCKKAPVKSCTECVRVDKDCAYCTDEMFRDRRCNTQAELLAAGCQRESIVVMESSFQITEETQIDTTLRRSQMSPQGLRVRLRPGEERHFELEVFEPLESPVDLYILMDFSNSMSDDLDNLKKMGQNLARVLSQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTEDVDEFRNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRPDSTHLLVFSTESAFHYEADGANVLAGIMSRNDERCHLDTTGTYTQYRTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHTYFPVSSLGVLQEDSSNIVELLEEAFNRIRSNLDIRALDSPRGLRTEVTSKMFQKTRTGSFHIRRGEVGIYQVQLRALEHVDGTHVCQLPEDQKGNIHLKPSFSDGLKMDAGIICDVCTCELQKEVRSARCSFNGDFVCGQCVCSEGWSGQTCNCSTGSLSDIQPCLREGEDKPCSGRGECQCGHCVCYGEGRYEGQFCEYDNFQCPRTSGFLCNDRGRCSMGQCVCEPGWTGPSCDCPLSNATCIDSNGGICNGRGHCECGRCHCHQQSLYTDTICEINYSAIHPGLCEDLRSCVQCQAWGTGEKKGRTCEECNFKVKMVDELKRAEEVVVRCSFRDEDDDCTYSYTMEGDGAPGPNSTVLVHKKKDCPPGSFWWLIPLLLLLLPLLALLLLLCWKYCACCKACLALLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPMLRSGNLKGRDVVRWKVTNNMQRPGFATHAASINPTELVPYGLSLRLARLCTENLLKPDTRECAQLRQEVEENLNEVYRQISGVHKLQQTKFRQQPNAGKKQDHTIVDTVLMAPRSAKPALLKLTEKQVEQRAFHDLKVAPGYYTLTADQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPAGTATLGRRLVNITIIKEQARDVVSFEQPEFSVSRGDQVARIPVIRRVLDGGKSQVSYRTQDGTAQGNRDYIPVEGELLFQPGEAWKELQVKLLELQEVDSLLRGRQVRRFHVQLSNPKFGAHLGQPHSTTIIIRDPDELDRSFTSQMLSSQPPPHGDLGAPQNPNAKAAGSRKIHFNWLPPSGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNDDNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQSGEDYDSFLMYSDDVLRSPSGSQRPSVSDDTGCGWKFEPLLGEELDLRRVTWRLPPELIPRLSASSGRSSDAEAPHGPPDDGGAGGKGGSLPRSATPGPPGEHLVNGRMDFAFPGSTNSLHRMTTTSAAAYGTHLSPHVPHRVLSTSSTLTRDYNSLTRSEHSHSTTLPRDYSTLTSVSSHDSRLTAGVPDTPTRLVFSALGPTSLRVSWQEPRCERPLQGYSVEYQLLNGGELHRLNIPNPAQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDIVGYLVTCEMAQGGGPATAFRVDGDSPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQDGGPFPQLGSRAGLFQHPLQSEYSSITTTHTSATEPFLVDGLTLGAQHLEAGGSLTRHVTQEFVSRTLTTSGTLSTHMDQQFFQT

PTMs - P16144 As Substrate

Site PTM Type Enzyme
S7 Phosphorylation
S21 Phosphorylation
Y50 Phosphorylation
S102 Phosphorylation
T164 Phosphorylation
S165 Phosphorylation
Y167 Phosphorylation
K173 Ubiquitination
K177 Ubiquitination
K191 Ubiquitination
T210 Phosphorylation
K219 Ubiquitination
S368 Phosphorylation
S377 Phosphorylation
T383 Phosphorylation
S387 Phosphorylation
K388 Ubiquitination
S397 Phosphorylation
Y408 Phosphorylation
K437 Sumoylation
K437 Ubiquitination
K662 Ubiquitination
T681 Phosphorylation
N695 N-Glycosylation
K758 Ubiquitination
Y762 Phosphorylation
S771 Phosphorylation
T776 Phosphorylation
K785 Ubiquitination
S809 Phosphorylation
S821 Phosphorylation
T829 Phosphorylation
K834 Ubiquitination
T837 Phosphorylation
Y855 Phosphorylation
K863 Ubiquitination
K868 Ubiquitination
T886 Phosphorylation
S893 Phosphorylation
K895 Ubiquitination
K904 Ubiquitination
K915 Ubiquitination
Y920 Phosphorylation
Y921 Phosphorylation
T922 Phosphorylation
K958 Ubiquitination
T971 Phosphorylation
T973 Phosphorylation
T982 Phosphorylation
S1000 Phosphorylation
K1021 Ubiquitination
Y1039 Phosphorylation
K1055 Ubiquitination
K1060 Ubiquitination
S1069 Phosphorylation
S1084 Phosphorylation
S1097 Phosphorylation
T1098 Phosphorylation
T1099 Phosphorylation
S1140 Phosphorylation
S1167 Phosphorylation
S1180 Phosphorylation
Y1187 Phosphorylation
Y1189 Phosphorylation
Y1199 Phosphorylation
Y1207 Phosphorylation
S1208 Phosphorylation
S1209 Phosphorylation
T1246 Phosphorylation
Y1257 Phosphorylation
T1297 Phosphorylation
T1318 Phosphorylation
S1325 Phosphorylation
Y1343 Phosphorylation
S1345 Phosphorylation
Y1349 Phosphorylation
S1350 Phosphorylation
S1356 Phosphorylation P27361 (MAPK3) , P17252 (PRKCA)
S1360 Phosphorylation P17252 (PRKCA)
S1364 Phosphorylation Q15418 (RPS6KA1) , P17612 (PRKACA) , P17252 (PRKCA)
S1403 Phosphorylation
S1428 Phosphorylation
T1434 Phosphorylation
S1454 Phosphorylation
T1455 Phosphorylation
S1457 Phosphorylation
S1465 Phosphorylation
Y1469 Phosphorylation
T1471 Phosphorylation
S1474 Phosphorylation
S1483 Phosphorylation
T1484 Phosphorylation
S1485 Phosphorylation
S1486 Phosphorylation
T1487 Phosphorylation
T1489 Phosphorylation
Y1492 Phosphorylation
S1494 Phosphorylation P17252 (PRKCA)
T1496 Phosphorylation
S1498 Phosphorylation
S1501 Phosphorylation
S1503 Phosphorylation
T1504 Phosphorylation
T1505 Phosphorylation
Y1510 Phosphorylation
S1511 Phosphorylation
T1512 Phosphorylation
T1514 Phosphorylation
S1515 Phosphorylation
S1517 Phosphorylation
S1518 Phosphorylation
S1521 Phosphorylation
T1524 Phosphorylation
T1530 Phosphorylation
S1537 Phosphorylation
S1547 Phosphorylation
Y1564 Phosphorylation P11362 (FGFR1)
S1595 Phosphorylation
Y1596 Phosphorylation
S1604 Phosphorylation
S1653 Phosphorylation
Y1674 Phosphorylation
T1688 Phosphorylation
S1696 Phosphorylation
S1707 Phosphorylation
T1720 Phosphorylation
T1721 Phosphorylation
S1747 Phosphorylation
S1758 Phosphorylation
Y1760 Phosphorylation
S1761 Phosphorylation
S1762 Phosphorylation
T1764 Phosphorylation
T1765 Phosphorylation
T1766 Phosphorylation
T1771 Phosphorylation
S1791 Phosphorylation
T1793 Phosphorylation
T1797 Phosphorylation
T1804 Phosphorylation
T1806 Phosphorylation
T1807 Phosphorylation

Research Backgrounds

Function:

Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling.

PTMs:

Palmitoylated by DHHC3 at several cysteines of the membrane-proximal region, enhancing stability and cell surface expression. Palmitoylation also promotes secondary association with tertaspanins.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cell membrane>Lipid-anchor. Cell junction>Hemidesmosome.
Note: Colocalizes with DST at the leading edge of migrating keratinocytes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.

Subunit Structure:

Heterodimer of an alpha and a beta subunit. Beta-4 associates with alpha-6. Interacts (via cytoplasmic region) with COL17A1 (via cytoplasmic region). Interacts (via cytoplasmic region) with DST isoform 3 (via N-terminus). Isoform beta-4a interacts (via cytoplasmic domain) with DST (via N-terminus). Interacts with RAC1. ITGA6:ITGB4 is found in a ternary complex with NRG1 and ERBB3. ITGA6:ITGB4 is found in a ternary complex with IGF1 and IGF1R. ITGA6:ITGB4 interacts with IGF2.

Family&Domains:

The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.

Belongs to the integrin beta chain family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

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