TIMP2 Antibody | Affinity Biosciences

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Western blot analysis on A549 cell lysates using TIMP2 Antibody,The lane on the left was treated with the antigen-specific peptide.

Product:	TIMP2 Antibody
Catalog:	AF0264
Description:	Rabbit polyclonal antibody to TIMP2
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Horse
Mol.Wt.:	24kDa; 24kD(Calculated).
Uniprot:	P16035
RRID:	AB_2833438

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Horse(100%)

Clonality:

Polyclonal

Specificity:

TIMP2 Antibody detects endogenous levels of total TIMP2.

RRID:

AB_2833438
Cite Format: Affinity Biosciences Cat# AF0264, RRID:AB_2833438.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CSC 21K; CSC-21K; CSC21K; Metalloproteinase inhibitor 2; Metalloproteinase inhibitor 2 precursor; TIMP 2; TIMP metallopeptidase inhibitor 2; TIMP-2; TIMP2; TIMP2_HUMAN; Tissue Inhibitor of Metalloproteinase 2; Tissue inhibitor of metalloproteinases 2;

Immunogens

Immunogen:

Uniprot:

P16035(TIMP2_HUMAN) >>Visit HPA database.

Gene(ID):

TIMP2(7077)

Description:

TIMP2 Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP13, MMP14, MMP15, MMP16 and MMP19.

Sequence:

P16035 TIMP2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGAAARTLRLALGLLLLATLLRPADACSCSPVHPQQAFCNADVVIRAKAVSEKEVDSGNDIYGNPIKRIQYEIKQIKMFKGPEKDIEFIYTAPSSAVCGVSLDVGGKKEYLIAGKAEGDGKMHITLCDFIVPWDTLSTTQKKSLNHRYQMGCECKITRCPMIPCYISSPDECLWMDWVTEKNINGHQAKFFACIKRSDGSCAWYRGAAPPKQEFLDIEDP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Pig

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16035 As Substrate

P16035

Site	PTM Type	Source
K53	Ubiquitination	Uniprot
S57	Phosphorylation	Uniprot
Y62	Phosphorylation	Uniprot
K67	Ubiquitination	Uniprot
K74	Ubiquitination	Uniprot
Y90	Phosphorylation	Uniprot
Y165	Phosphorylation	Uniprot

Research Backgrounds

P16035_TIMP2_HUMAN

Function:

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.

PTMs:

The activity of TIMP2 is dependent on the presence of disulfide bonds.

Subcellular Location:

Secreted.

Subunit Structure:

Interacts (via the C-terminal) with MMP2 (via the C-terminal PEX domain); the interaction inhibits the MMP2 activity.

Family&Domains:

Belongs to the protease inhibitor I35 (TIMP) family.

References

1). Quercetin induces MGMT+ glioblastoma cells apoptosis via dual inhibition of Wnt3a/β-Catenin and Akt/NF-κB signaling pathways. Phytomedicine, 2023 (PubMed: 37451151) [IF=7.9]

2). Low-Frequency Vibration Promotes Tumor Necrosis Factor-α Production to Increase Cartilage Degeneration in Knee Osteoarthritis. Cartilage, 2021 (PubMed: 32532183) [IF=2.8]

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For Research Use Only.
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TIMP2 Antibody - #AF0264