Synaptotagmin 1/2 Antibody - #AF6223

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Product: Synaptotagmin 1/2 Antibody
Catalog: AF6223
Description: Rabbit polyclonal antibody to Synaptotagmin 1/2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 65kDa; 48kD,47kD(Calculated).
Uniprot: P21579 | Q8N9I0
RRID: AB_2835040

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
Synaptotagmin 1/2 Antibody detects endogenous levels of total Synaptotagmin 1/2.
RRID:
AB_2835040
Cite Format: Affinity Biosciences Cat# AF6223, RRID:AB_2835040.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp781D2042; FLJ42519; p65; SVP65; Synaptotagmin 2; Synaptotagmin I; Synaptotagmin II; Synaptotagmin-1; SYT; Syt1; SYT1_HUMAN; Syt2; SytI; Synaptotagmin 2; Synaptotagmin II; Synaptotagmin-2; Synaptotagmin2; SynaptotagminII; SYT 2; Syt II; Syt2; SYT2_HUMAN; SytII;

Immunogens

Immunogen:
Uniprot:

P21579(SYT1_HUMAN) >>Visit HPA database.

Q8N9I0(SYT2_HUMAN) >>Visit HPA database.

Gene(ID):
SYT1(6857) | SYT2(127833)
Expression:
P21579 SYT1_HUMAN:

Expressed in melanocytes (PubMed:23999003).

Q8N9I0 SYT2_HUMAN:

Expressed in melanocytes (PubMed:23999003).

Description:
SYT2 May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.
Sequence:
MVSESHHEALAAPPVTTVATVLPSNATEPASPGEGKEDAFSKLKEKFMNELHKIPLPPWALIAIAIVAVLLVLTCCFCICKKCLFKKKNKKKGKEKGGKNAINMKDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEEEVDAMLAVKK

MRNIFKRNQEPIVAPATTTATMPIGPVDNSTESGGAGESQEDMFAKLKEKLFNEINKIPLPPWALIAIAVVAGLLLLTCCFCICKKCCCKKKKNKKEKGKGMKNAMNMKDMKGGQDDDDAETGLTEGEGEGEEEKEPENLGKLQFSLDYDFQANQLTVGVLQAAELPALDMGGTSDPYVKVFLLPDKKKKYETKVHRKTLNPAFNETFTFKVPYQELGGKTLVMAIYDFDRFSKHDIIGEVKVPMNTVDLGQPIEEWRDLQGGEKEEPEKLGDICTSLRYVPTAGKLTVCILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTVKKKTLNPYFNESFSFEIPFEQIQKVQVVVTVLDYDKLGKNEAIGKIFVGSNATGTELRHWSDMLANPRRPIAQWHSLKPEEEVDALLGKNK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
80
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P21579/Q8N9I0 As Substrate

Site PTM Type Enzyme
S24 Phosphorylation
K120 Ubiquitination
T126 Phosphorylation
T129 Phosphorylation
K134 Ubiquitination
Y181 Phosphorylation
T202 Phosphorylation
T212 Phosphorylation
Y217 Phosphorylation
Y230 Phosphorylation
K237 Acetylation
K302 Acetylation
Y312 Phosphorylation
K333 Ubiquitination
Site PTM Type Enzyme
K46 Acetylation
K48 Acetylation
K98 Ubiquitination
T122 Phosphorylation
T125 Phosphorylation
Y178 Phosphorylation
T199 Phosphorylation Q9H4A3 (WNK1)
K299 Acetylation
Y309 Phosphorylation
S378 Phosphorylation
T381 Phosphorylation
T383 Phosphorylation Q9H4A3 (WNK1)

Research Backgrounds

Function:

Calcium sensor that participates in triggering neurotransmitter release at the synapse (By similarity). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (By similarity). It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes.

PTMs:

Glycosylated.

Subcellular Location:

Cytoplasmic vesicle>Secretory vesicle membrane>Single-pass membrane protein. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane>Single-pass membrane protein. Cytoplasmic vesicle>Secretory vesicle>Chromaffin granule membrane>Single-pass membrane protein. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in melanocytes.

Subunit Structure:

Homotetramer (Probable). Interacts with SCAMP5. Interacts with STON2. Forms a complex with SV2B, syntaxin 1 and SNAP25 (By similarity). Interacts with SV2A, SV2B and SV2C (By similarity). Interacts with RIMS1 (By similarity). Interacts with PRRT2 (By similarity). Interacts with DNAJC5 in a phosphorylation-dependent manner (By similarity). Interacts (via N-terminus) with RAB3A (By similarity). Interacts with SYT12 (By similarity). Interacts with calmodulin (By similarity).

Family&Domains:

The first C2 domain mediates Ca(2+)-dependent phospholipid binding.

The second C2 domain mediates interaction with SV2A and probably with STN2.

Belongs to the synaptotagmin family.

Function:

Exhibits calcium-dependent phospholipid and inositol polyphosphate binding properties (By similarity). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (By similarity). Plays a role in dendrite formation by melanocytes.

Subcellular Location:

Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane>Single-pass membrane protein. Cytoplasmic vesicle>Secretory vesicle>Chromaffin granule membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in melanocytes.

Subunit Structure:

Homotetramer (Probable). Interacts with STON2. Interacts with SCAMP5. Interacts with PRRT2 (By similarity).

Family&Domains:

The first C2 domain mediates Ca(2+)-dependent phospholipid binding.

The second C2 domain mediates interaction with Stonin 2. The second C2 domain mediates phospholipid and inositol polyphosphate binding in a calcium-independent manner.

Belongs to the synaptotagmin family.

Research Fields

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

References

1). Fibroblast growth factor 2 contributes to the effect of salidroside on dendritic and synaptic plasticity after cerebral ischemia/reperfusion injury. Aging (Albany NY), 2020 (PubMed: 32518214) [IF=5.2]

Application: WB    Species: rat    Sample:

Figure 10.| Sal promotes increases in dendritic spine density and synaptic-associated protein expression via upregulating the FGF2-mediated cAMP/PKA/CREB signaling pathway.(E–H) Representative western blot bands of PSD95, synapsin I and synaptotagmin in each group. (I) QPCR data for PSD95. (J) Transmission electron microscopy showed the synaptic structures (the scale bar is 1 μm). Values are expressed as the mean ± SD. #p < 0.05, ##p < 0.01 vs. sham; *p < 0.05, **p < 0.01 vs. MCAO/R.
2). Electroacupuncture may alleviate neuropathic pain via suppressing P2X7R expression. Molecular Pain, 2021 (PubMed: 33626989) [IF=3.3]

Application: WB    Species: rats    Sample: spinal dorsal horn

Figure 2. EA reduced abnormal remodeling of dendritic spines/synapses, whereas BzATP had the opposite effects. (a) and (b) Representative Western blots and quantification data of the expression of Synaptotagmin 1/GAPDH. (n ¼ 3). (c) Dendritic spines. Scale bars, 10 lm. (d) Number of spines per 10 lm. (n ¼ 3). (e) The ultrastructure of synapses in the spinal dorsal horn from rats in each group (0.2 lm, 30,000 ). (f) Number of synapses per 30000x field. (n ¼ 4). (g) Width of synaptic space (nm). (n ¼ 4). (h) Vesicle density (vesicles/ mm2 ). (n ¼ 4). Columns represent the mean  SD. #p < 0.05 and ##p < 0.01 vs. the S group; $p < 0.05 and $$p < 0.01 vs. the M group; &&p < 0.01 vs. the MþEA group.

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