SCD1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Hela cells(heat shock treatment), using SCD1 Antibody.

Product:	SCD1 Antibody
Catalog:	AF6781
Description:	Rabbit polyclonal antibody to SCD1
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	41 kDa; 42kD(Calculated).
Uniprot:	O00767
RRID:	AB_2847504

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

SCD1 Antibody detects endogenous levels of total SCD1.

RRID:

AB_2847504
Cite Format: Affinity Biosciences Cat# AF6781, RRID:AB_2847504.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACOD_HUMAN; Acyl-CoA desaturase; Delta(9)-desaturase; Delta-9 desaturase; Delta-9-Desaturase; FADS5; Fatty acid desaturase; PRO0998; SCD 1; SCD; SCD1; Stearoyl-CoA desaturase;

Immunogens

Immunogen:

A synthesized peptide derived from human SCD1.

Uniprot:

O00767(ACOD_HUMAN) >>Visit HPA database.

Gene(ID):

SCD(6319)

Expression:

O00767 ACOD_HUMAN:

Detected in fetal liver, lung and brain. Highly expressed in adult adipose tissue, and at lower levels in adult brain and lung.

Sequence:

O00767 ACOD_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPAHLLQDDISSSYTTTTTITAPPSRVLQNGGDKLETMPLYLEDDIRPDIKDDIYDPTYKDKEGPSPKVEYVWRNIILMSLLHLGALYGITLIPTCKFYTWLWGVFYYFVSALGITAGAHRLWSHRSYKARLPLRLFLIIANTMAFQNDVYEWARDHRAHHKFSETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGSTLDLSDLEAEKLVMFQRRYYKPGLLMMCFILPTLVPWYFWGETFQNSVFVATFLRYAVVLNATWLVNSAAHLFGYRPYDKNISPRENILVSLGAVGEGFHNYHHSFPYDYSASEYRWHINFTTFFIDCMAALGLAYDRKKVSKAAILARIKRTGDGNYKSG

PTMs - O00767 As Substrate

O00767

Site	PTM Type	Source
Y14	Phosphorylation	Uniprot
K34	Ubiquitination	Uniprot
Y41	Phosphorylation	Uniprot
K51	Sumoylation	Uniprot
K51	Ubiquitination	Uniprot
Y55	Phosphorylation	Uniprot
Y59	Phosphorylation	Uniprot
K60	Ubiquitination	Uniprot
K62	Ubiquitination	Uniprot
K68	Sumoylation	Uniprot
K68	Ubiquitination	Uniprot
S80	Phosphorylation	Uniprot
T91	Phosphorylation	Uniprot
T95	Phosphorylation	Uniprot
K162	Ubiquitination	Uniprot
K189	Ubiquitination	Uniprot
K194	Ubiquitination	Uniprot
K196	Ubiquitination	Uniprot
S198	Phosphorylation	Uniprot
T199	Phosphorylation	Uniprot
S203	Phosphorylation	Uniprot
K209	Ubiquitination	Uniprot
K278	Ubiquitination	Uniprot
K338	Ubiquitination	Uniprot
K341	Ubiquitination	Uniprot
T351	Phosphorylation	Uniprot
K357	Ubiquitination	Uniprot

Research Backgrounds

O00767_ACOD_HUMAN

Function:

Stearyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates. Catalyzes the insertion of a cis double bond at the delta-9 position into fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Gives rise to a mixture of 16:1 and 18:1 unsaturated fatty acids. Plays an important role in lipid biosynthesis. Plays an important role in regulating the expression of genes that are involved in lipogenesis and in regulating mitochondrial fatty acid oxidation (By similarity). Plays an important role in body energy homeostasis (By similarity). Contributes to the biosynthesis of membrane phospholipids, cholesterol esters and triglycerides (By similarity).

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Tissue Specificity:

Detected in fetal liver, lung and brain. Highly expressed in adult adipose tissue, and at lower levels in adult brain and lung.

Subunit Structure:

May self-associate and form homodimers.

Family&Domains:

The histidine box domains are involved in binding the catalytic metal ions.

Belongs to the fatty acid desaturase type 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Metabolism > Lipid metabolism > Biosynthesis of unsaturated fatty acids.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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SCD1 Antibody - #AF6781