CRYAB Antibody | Affinity Biosciences

Product:	CRYAB Antibody
Catalog:	AF6754
Description:	Rabbit polyclonal antibody to CRYAB
Application:	ELISA(peptide)
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	20kD; 20kD(Calculated).
Uniprot:	P02511
RRID:	AB_2847477

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Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

CRYAB Antibody detects endogenous levels of total CRYAB.

RRID:

AB_2847477
Cite Format: Affinity Biosciences Cat# AF6754, RRID:AB_2847477.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AACRYA; Alpha B crystallin; Alpha crystallin B chain; Alpha(B)-crystallin; Alpha-crystallin B chain; CRYA2; Cryab; CRYAB_HUMAN; Crystallin alpha B; Crystallin alpha polypeptide 2; CTPP2; Heat shock 20 kD like protein; Heat shock protein beta 5; Heat shock protein beta-5; HspB5; Renal carcinoma antigen NY REN 27; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component;

Immunogens

Immunogen:

A synthesized peptide derived from human CRYAB.

Uniprot:

P02511(CRYAB_HUMAN) >>Visit HPA database.

Gene(ID):

CRYAB(1410)

Expression:

P02511 CRYAB_HUMAN:

Lens as well as other tissues (PubMed:838078, PubMed:2387586). Expressed in myocardial tissue (PubMed:28493373).

Sequence:

P02511 CRYAB_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDIAIHHPWIRRPFFPFHSPSRLFDQFFGEHLLESDLFPTSTSLSPFYLRPPSFLRAPSWFDTGLSEMRLEKDRFSVNLDVKHFSPEELKVKVLGDVIEVHGKHEERQDEHGFISREFHRKYRIPADVDPLTITSSLSSDGVLTVNGPRKQVSGPERTIPITREEKPAVTAAPKK

PTMs - P02511 As Substrate

P02511

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
S19	Phosphorylation		Uniprot
S21	Phosphorylation		Uniprot
R22	Methylation		Uniprot
S43	Phosphorylation		Uniprot
S45	Phosphorylation		Uniprot
R50	Methylation		Uniprot
S53	Phosphorylation		Uniprot
S59	Phosphorylation	Q16539 (MAPK14)	Uniprot
R74	Methylation		Uniprot
S76	Phosphorylation		Uniprot
K82	Ubiquitination		Uniprot
S85	Phosphorylation		Uniprot
K90	Acetylation		Uniprot
K90	Ubiquitination		Uniprot
K92	Acetylation		Uniprot
K92	Ubiquitination		Uniprot
T132	Phosphorylation		Uniprot
T134	Phosphorylation		Uniprot
S135	Phosphorylation		Uniprot
S136	Phosphorylation		Uniprot
T144	Phosphorylation		Uniprot
K150	Ubiquitination		Uniprot
T158	Phosphorylation		Uniprot
K166	Acetylation		Uniprot
K166	Ubiquitination		Uniprot
T170	O-Glycosylation		Uniprot

Research Backgrounds

P02511_CRYAB_HUMAN

Function:

May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles (PubMed:19464326). Localizes at the Z-bands and the intercalated disk in cardiomyocytes (PubMed:28493373).

Tissue Specificity:

Lens as well as other tissues. Expressed in myocardial tissue.

Subunit Structure:

Heteropolymer composed of three CRYAA and one CRYAB subunits. Aggregates with homologous proteins, including the small heat shock protein HSPB1, to form large heteromeric complexes. Inter-subunit bridging via zinc ions enhances stability, which is crucial as there is no protein turn over in the lens. Interacts with HSPBAP1 and TTN/titin. Interacts with TMEM109. Interacts with DES; binds rapidly during early stages of DES filament assembly and a reduced binding seen in the later stages.

Family&Domains:

Belongs to the small heat shock protein (HSP20) family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species. (View pathway)

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For Research Use Only.
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CRYAB Antibody - #AF6754