PKA R2 Antibody - #AF6724

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Product: PKA R2 Antibody
Catalog: AF6724
Description: Rabbit polyclonal antibody to PKA R2
Application: IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 50kDa; 46kD(Calculated).
Uniprot: P13861
RRID: AB_2847447

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
PKA R2 Antibody detects endogenous levels of total PKA R2.
RRID:
AB_2847447
Cite Format: Affinity Biosciences Cat# AF6724, RRID:AB_2847447.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cAMP dependent protein kinase regulatory subunit alpha 2; cAMP dependent protein kinase regulatory subunit RII alpha; cAMP dependent protein kinase type II alpha regulatory chain; cAMP dependent protein kinase type II alpha regulatory subunit; cAMP-dependent protein kinase type II-alpha regulatory subunit; KAP2; KAP2_HUMAN; MGC3606; PKR 2; PKR2; PRKA R2; PRKAR 2; PRKAR2; PRKAR2A; Protein kinase A RII alpha subunit; Protein kinase cAMP dependent regulatory type II alpha;

Immunogens

Immunogen:

A synthesized peptide derived from human PKA R2.

Uniprot:

P13861(KAP2_HUMAN) >>Visit HPA database.

Gene(ID):
PRKAR2A(5576)
Expression:
P13861 KAP2_HUMAN:

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Sequence:
MSHIQIPPGLTELLQGYTVEVLRQQPPDLVEFAVEYFTRLREARAPASVLPAATPRQSLGHPPPEPGPDRVADAKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQGEKADSFYIIESGEVSILIRSRTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ

PTMs - P13861 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S48 Phosphorylation
T54 Phosphorylation
S58 Phosphorylation
S78 Phosphorylation
S80 Phosphorylation
S92 Phosphorylation
S99 Phosphorylation
C101 S-Nitrosylation
T104 Phosphorylation
Y105 Phosphorylation
T114 Phosphorylation
K122 Ubiquitination
K135 Ubiquitination
K161 Ubiquitination
Y177 Phosphorylation
K190 Ubiquitination
S196 Phosphorylation
Y200 Phosphorylation
S254 Phosphorylation
K263 Ubiquitination
S268 Phosphorylation
K272 Ubiquitination
K280 Ubiquitination
K315 Methylation
K318 Acetylation
K318 Ubiquitination
K332 Ubiquitination
T343 Phosphorylation
K345 Ubiquitination
S350 Phosphorylation
Y352 Phosphorylation
S383 Phosphorylation
Y385 Phosphorylation
S395 Phosphorylation
S396 Phosphorylation

PTMs - P13861 As Enzyme

Substrate Site Source
P13861-1 (PRKAR2A) S99 Uniprot

Research Backgrounds

Function:

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

PTMs:

Phosphorylated by the activated catalytic chain.

Subcellular Location:

Cytoplasm. Cell membrane.
Note: Colocalizes with PJA2 in the cytoplasm and the cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Subunit Structure:

The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4 and CBFA2T3. Interacts with the phosphorylated form of PJA2. Interacts with MYRIP. This interaction may link PKA to components of the exocytosis machinery, thus facilitating exocytosis, including insulin release (By similarity). Forms a complex composed of PRKAR2A, GSK3B and GSKIP through GSKIP interaction; facilitates PKA-induced phosphorylation and regulates GSK3B activity. Interacts with ADCY8; inhibits adenylate cyclase activity through PKA phosphorylation.

Family&Domains:

Belongs to the cAMP-dependent kinase regulatory chain family.

Research Fields

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

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