SRF Antibody | Affinity Biosciences

IF/ICC
Cites

1/4

AF6160 staining HepG2 cells by IF/ICC.

Figure 5.

Figure 5 KIF3A knockdown increases α-SMA-positive myofibroblasts among SiO2-activated MRC-5 fibroblasts.

Fig.

Product:	SRF Antibody
Catalog:	AF6160
Description:	Rabbit polyclonal antibody to SRF
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Rabbit, Dog
Mol.Wt.:	67kDa; 52kD(Calculated).
Uniprot:	P11831
RRID:	AB_2835029

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

SRF Antibody detects endogenous levels of total SRF.

RRID:

AB_2835029
Cite Format: Affinity Biosciences Cat# AF6160, RRID:AB_2835029.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

c fos serum response element binding factor; c fos serum response element binding transcription factor; ELK3; ERP; MCM 1; MCM1; OTTHUMP00000039820; SAP2; Serum response factor; SRF; SRF serum response factor c fos serum response element binding transcription factor; SRF_HUMAN;

Immunogens

Immunogen:

Uniprot:

P11831(SRF_HUMAN) >>Visit HPA database.

Gene(ID):

SRF(6722)

Description:

This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes.

Sequence:

P11831 SRF_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLPTQAGAAAALGRGSALGGSLNRTPTGRPGGGGGTRGANGGRVPGNGAGLGPGRLEREAAAAAATTPAPTAGALYSGSEGDSESGEEEELGAERRGLKRSLSEMEIGMVVGGPEASAAATGGYGPVSGAVSGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVLLLVASETGHVYTFATRKLQPMITSETGKALIQTCLNSPDSPPRSDPTTDQRMSATGFEETDLTYQVSESDSSGETKDTLKPAFTVTNLPGTTSTIQTAPSTSTTMQVSSGPSFPITNYLAPVSASVSPSAVSSANGTVLKSTGSGPVSSGGLMQLPTSFTLMPGGAVAQQVPVQAIQVHQAPQQASPSRDSSTDLTQTSSSGTVTLPATIMTSSVPTTVGGHMMYPSPHAVMYAPTSGLGDGSLTVLNAFSQAPSTMQVSHSQVQEPGGVPQVFLTASSGTVQIPVSAVQLHQMAVIGQQAGSSSNLTELQVVNLDTAHSTKSE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Dog

100

Rabbit

100

Xenopus

Horse

Sheep

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11831 As Substrate

P11831

Site	PTM Type	Enzyme	Source
T4	Phosphorylation		Uniprot
R14	Methylation		Uniprot
S16	Phosphorylation		Uniprot
S21	Phosphorylation		Uniprot
R24	Methylation		Uniprot
T27	Phosphorylation		Uniprot
R37	Methylation		Uniprot
T71	Phosphorylation		Uniprot
Y76	Phosphorylation		Uniprot
S77	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S79	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S83	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S85	Phosphorylation	P49137 (MAPKAPK2) , P68400 (CSNK2A1)	Uniprot
S101	Phosphorylation		Uniprot
S103	Phosphorylation	Q15418 (RPS6KA1) , O75582 (RPS6KA5) , Q9UQM7 (CAMK2A) , P49137 (MAPKAPK2)	Uniprot
K147	Sumoylation		Uniprot
T159	Phosphorylation	P17612 (PRKACA) , Q13976 (PRKG1) , Q09013 (DMPK) , P17252 (PRKCA)	Uniprot
T160	Phosphorylation	Q9UQM7 (CAMK2A) , Q05655 (PRKCD)	Uniprot
S162	Phosphorylation	P17252 (PRKCA)	Uniprot
S176	Phosphorylation		Uniprot
T177	Phosphorylation		Uniprot
T199	Phosphorylation		Uniprot
S221	Phosphorylation		Uniprot
S224	Phosphorylation		Uniprot
S228	Phosphorylation		Uniprot
S251	Phosphorylation		Uniprot
S253	Phosphorylation		Uniprot
S277	O-Glycosylation		Uniprot
S307	O-Glycosylation		Uniprot
S309	O-Glycosylation		Uniprot
S313	O-Glycosylation		Uniprot
S316	O-Glycosylation		Uniprot
S370	Phosphorylation		Uniprot
S383	O-Glycosylation		Uniprot
T401	O-Glycosylation		Uniprot
S435	Phosphorylation	P78527 (PRKDC)	Uniprot
S446	Phosphorylation	P78527 (PRKDC)	Uniprot

Research Backgrounds

P11831_SRF_HUMAN

Function:

SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation.

PTMs:

Phosphorylated by PRKDC.

Subcellular Location:

Nucleus.

Subunit Structure:

Binds DNA as a multimer, probably a dimer. Interacts with MRTFA, forming the SRF-MRTFA nuclear complex which binds the 5'-CArG-3' consensus motif (CArG box) on DNA via SRF. Forms a nuclear ternary complex with MRTFA and SCAI. Interacts with MRTFB. Interacts with MLLT7/FOXO4, NKX3A and SSRP1. Interacts with ARID2 (By similarity). Interacts with SRFBP1 (By similarity). Interacts with FOXK1. Interacts with LPXN. Interacts with OLFM2; the interaction promotes dissociation of SRF from the transcriptional repressor HEY2, facilitates binding of SRF to target genes and promotes smooth muscle differentiation.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

References

1). Silica Perturbs Primary Cilia and Causes Myofibroblast Differentiation during Silicosis by Reduction of the KIF3A-Repressor GLI3 Complex. Theranostics, 2023 (PubMed: 32042332) [IF=12.4]

Application: WB Species: human Sample: MRC-5 fibroblasts

Figure 5. KIF3A knockdown increases α-SMA-positive myofibroblasts among SiO2-activated MRC-5 fibroblasts. (A) Western blot showing the effects of NC-siRNA and KIF3A-siRNA on expression of KIF3A, Ac-α-Tub, and ARL13B proteins in MRC-5 fibroblasts. GAPDH was used as a loading control (n=3). (B) Densitometric analyses of KIF3A, Ac-α-Tub, and ARL13B protein expression in MRC-5 fibroblasts. *P<0.05; **P<0.01. Data are the mean±SD. Statistical analysis was performed using one-way ANOVA and SPSS 20.0. (C) IF assay showing primary cilia in MRC-5 fibroblasts treated with NC-siRNA or KIF3A-siRNA. Primary cilia were labelled with an anti-Ac-α-Tub antibody. Scale bar=25 and 5 μm. (D) Treatment regimen of KIF3A knockdown in MRC-5 fibroblasts. MRC-5 fibroblasts were stimulated with SiO2 or serum-free medium (n=3 per group) for 12 h, and then transfected with NC-siRNA or KIF3A-siRNA until 36 h. (E) Expression of α-SMA in MRC-5 fibroblasts measured by IF. Scale bar=100 μm. (F, G) Western blot and densitometric analyses of the effects of NC-siRNA and KIF3A-siRNA on expression of COL I, α-SMA, MRTF-A and SRF proteins in MRC-5 fibroblasts with or without SiO2 stimulation. α-Tub was used as a loading control (n=3). *P<0.05; **P<0.01. Data are the mean±SD. Statistical analysis was performed using one-way ANOVA and SPSS 20.0.

Application: WB Species: Human Sample: MRC-5 fibroblasts

Figure 5 KIF3A knockdown increases α-SMA-positive myofibroblasts among SiO2-activated MRC-5 fibroblasts. (A) Western blot showing the effects of NC-siRNA and KIF3A-siRNA on expression of KIF3A, Ac-α-Tub, and ARL13B proteins in MRC-5 fibroblasts. GAPDH was used as a loading control (n=3). (B) Densitometric analyses of KIF3A, Ac-α-Tub, and ARL13B protein expression in MRC-5 fibroblasts. *P<0.05; **P<0.01. Data are the mean±SD. Statistical analysis was performed using one-way ANOVA and SPSS 20.0. (C) IF assay showing primary cilia in MRC-5 fibroblasts treated with NC-siRNA or KIF3A-siRNA. Primary cilia were labelled with an anti-Ac-α-Tub antibody. Scale bar=25 and 5 μm. (D) Treatment regimen of KIF3A knockdown in MRC-5 fibroblasts. MRC-5 fibroblasts were stimulated with SiO2 or serum-free medium (n=3 per group) for 12 h, and then transfected with NC-siRNA or KIF3A-siRNA until 36 h. (E) Expression of α-SMA in MRC-5 fibroblasts measured by IF. Scale bar=100 μm. (F, G) Western blot and densitometric analyses of the effects of NC-siRNA and KIF3A-siRNA on expression of COL I, α-SMA, MRTF-A and SRF proteins in MRC-5 fibroblasts with or without SiO2 stimulation. α-Tub was used as a loading control (n=3). *P<0.05; **P<0.01. Data are the mean±SD. Statistical analysis was performed using one-way ANOVA and SPSS 20.0.

2). Ac-SDKP promotes KIF3A-mediated β-catenin suppression through a ciliary mechanism to constrain silica-induced epithelial-myofibroblast transition. Biomedicine & Pharmacotherapy, 2023 (PubMed: 37651800) [IF=7.5]

Application: WB Species: Rat Sample:

Fig. 1. Ac-SDKP promotes extension of primary cilia and inhibits EMyT in silicotic rats (A) Sirius red staining of lung tissues and the expression levels of KIF3A measured by IHC staining in rats exposed to silica, with or without Ac-SDKP intervention (scale bar = 100 µm). (B) Primary cilia in lung tissue observed by IF staining. The primary cilia were marked by ARL13B (green), and the silicitic nodules were marked by vimentin (red) (scale bar = 50 mm). (C) Protein expression levels of COL I, α-SMA, and E-cadherin in rats exposed to silica with or without Ac-SDKP. Quantification of the Western blots normalized to the loading control, α-Tub; data are presented as mean ± SD.

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SRF Antibody - #AF6160