mGluR5 Antibody | Affinity Biosciences

Product:	mGluR5 Antibody
Catalog:	AF6669
Description:	Rabbit polyclonal antibody to mGluR5
Application:	ELISA(peptide)
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	132kD(Calculated).
Uniprot:	P41594
RRID:	AB_2847392

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Secondary Antibodies
Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

mGluR5 Antibody detects endogenous levels of total mGluR5.

RRID:

AB_2847392
Cite Format: Affinity Biosciences Cat# AF6669, RRID:AB_2847392.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Glutamate receptor metabotropic 5; GPRC1E; Grm5; GRM5_HUMAN; Metabotropic glutamate receptor 5; Metabotropic glutamate receptor 5 variant F; Metabotropic glutamate receptor 5 variant G; Metabotropic glutamate receptor 5 variant H; mGlu5; mGluR5; PPP1R86; Protein phosphatase 1 regulatory subunit 86;

Immunogens

Immunogen:

A synthesized peptide derived from human mGluR5.

Uniprot:

P41594(GRM5_HUMAN) >>Visit HPA database.

Gene(ID):

GRM5(2915)

Sequence:

P41594 GRM5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVLLLILSVLLLKEDVRGSAQSSERRVVAHMPGDIIIGALFSVHHQPTVDKVHERKCGAVREQYGIQRVEAMLHTLERINSDPTLLPNITLGCEIRDSCWHSAVALEQSIEFIRDSLISSEEEEGLVRCVDGSSSSFRSKKPIVGVIGPGSSSVAIQVQNLLQLFNIPQIAYSATSMDLSDKTLFKYFMRVVPSDAQQARAMVDIVKRYNWTYVSAVHTEGNYGESGMEAFKDMSAKEGICIAHSYKIYSNAGEQSFDKLLKKLTSHLPKARVVACFCEGMTVRGLLMAMRRLGLAGEFLLLGSDGWADRYDVTDGYQREAVGGITIKLQSPDVKWFDDYYLKLRPETNHRNPWFQEFWQHRFQCRLEGFPQENSKYNKTCNSSLTLKTHHVQDSKMGFVINAIYSMAYGLHNMQMSLCPGYAGLCDAMKPIDGRKLLESLMKTNFTGVSGDTILFDENGDSPGRYEIMNFKEMGKDYFDYINVGSWDNGELKMDDDEVWSKKSNIIRSVCSEPCEKGQIKVIRKGEVSCCWTCTPCKENEYVFDEYTCKACQLGSWPTDDLTGCDLIPVQYLRWGDPEPIAAVVFACLGLLATLFVTVVFIIYRDTPVVKSSSRELCYIILAGICLGYLCTFCLIAKPKQIYCYLQRIGIGLSPAMSYSALVTKTNRIARILAGSKKKICTKKPRFMSACAQLVIAFILICIQLGIIVALFIMEPPDIMHDYPSIREVYLICNTTNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITMCFSVSLSATVALGCMFVPKVYIILAKPERNVRSAFTTSTVVRMHVGDGKSSSAASRSSSLVNLWKRRGSSGETLRYKDRRLAQHKSEIECFTPKGSMGNGGRATMSSSNGKSVTWAQNEKSSRGQHLWQRLSIHINKKENPNQTAVIKPFPKSTESRGLGAGAGAGGSAGGVGATGGAGCAGAGPGGPESPDAGPKALYDVAEAEEHFPAPARPRSPSPISTLSHRAGSASRTDDDVPSLHSEPVARSSSSQGSLMEQISSVVTRFTANISELNSMMLSTAAPSPGVGAPLCSSYLIPKEIQLPTTMTTFAEIQPLPAIEVTGGAQPAAGAQAAGDAARESPAAGPEAAAAKPDLEELVALTPPSPFRDSVDSGSTTPNSPVSESALCIPSSPKYDTLIIRDYTQSSSSL

PTMs - P41594 As Substrate

P41594

Site	PTM Type	Source
S8	Phosphorylation	Uniprot
S23	Phosphorylation	Uniprot
S98	Phosphorylation	Uniprot
S250	Phosphorylation	Uniprot
S256	Phosphorylation	Uniprot
K259	Ubiquitination	Uniprot
S266	Phosphorylation	Uniprot
T282	Phosphorylation	Uniprot
T607	Phosphorylation	Uniprot
Y619	Phosphorylation	Uniprot
S654	Phosphorylation	Uniprot
K828	Acetylation	Uniprot
S888	Phosphorylation	Uniprot
T894	Phosphorylation	Uniprot
S955	Phosphorylation	Uniprot
T956	Phosphorylation	Uniprot
S958	Phosphorylation	Uniprot
S970	Phosphorylation	Uniprot
T977	Phosphorylation	Uniprot
Y1001	Phosphorylation	Uniprot
S1018	Phosphorylation	Uniprot
S1020	Phosphorylation	Uniprot

Research Backgrounds

P41594_GRM5_HUMAN

Function:

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Subunit Structure:

The PPXXF motif binds HOMER1, HOMER2 and HOMER3. Interacts with SIAH1, RYR1, RYR2, ITPR1, SHANK1, SHANK3 and GRASP. Interacts with NCDN (By similarity). Isoform 2 interacts with NECAB2. Interacts with CAMK2A.

Family&Domains:

Belongs to the G-protein coupled receptor 3 family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

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For Research Use Only.
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mGluR5 Antibody - #AF6669