Product: BACE1 Antibody
Catalog: AF6650
Description: Rabbit polyclonal antibody to BACE1
Application: ELISA(peptide)
Reactivity: Human, Mouse, Rat
Mol.Wt.: 56kD(Calculated).
Uniprot: P56817
RRID: AB_2847373

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Product Info

Source:
Rabbit
Application:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
BACE1 Antibody detects endogenous levels of total BACE1.
RRID:
AB_2847373
Cite Format: Affinity Biosciences Cat# AF6650, RRID:AB_2847373.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

APP beta secretase; Asp 2; ASP2; Aspartyl protease 2; BACE 1; BACE; BACE1; BACE1_HUMAN; Beta secretase 1; Beta secretase; Beta site amyloid beta A4 precursor protein cleaving enzyme; Beta site amyloid precursor protein cleaving enzyme 1; Beta site amyloid precursor protein cleaving enzyme; Beta site APP cleaving enzyme 1; Beta site APP cleaving enzyme; Beta-secretase 1; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; FLJ90568; HSPC104; Memapsin 2; Memapsin-2; Memapsin2; Membrane associated aspartic protease 2; Membrane-associated aspartic protease 2; Transmembrane aspartic proteinase Asp2;

Immunogens

Immunogen:

A synthesized peptide derived from human BACE1.

Uniprot:
Gene(ID):
Expression:
P56817 BACE1_HUMAN:

Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.

Sequence:
MAQALPWLLLWMGAGVLPAHGTQHGIRLPLRSGLGGAPLGLRLPRETDEEPEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRQQHDDFADDISLLK

PTMs - P56817 As Substrate

Site PTM Type Enzyme
T47 Phosphorylation
S59 Phosphorylation
S71 Phosphorylation
S83 Phosphorylation
K126 Acetylation
K136 Acetylation
T252 Phosphorylation Q00535 (CDK5)
Y260 Phosphorylation
K275 Acetylation
K279 Acetylation
K285 Acetylation
K285 Ubiquitination
K299 Acetylation
K300 Acetylation
K300 Ubiquitination
K307 Acetylation
S308 Phosphorylation
S498 Phosphorylation P48729 (CSNK1A1) , O75116 (ROCK2) , P48730 (CSNK1D)
K501 Ubiquitination

Research Backgrounds

Function:

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. Cleaves CHL1 (By similarity).

PTMs:

N-Glycosylated. Addition of a bisecting N-acetylglucosamine by MGAT3 blocks lysosomal targeting, further degradation and is required for maintaining stability under stress conditions (By similarity).

Acetylated in the endoplasmic reticulum at Lys-126, Lys-275, Lys-279, Lys-285, Lys-299, Lys-300 and Lys-307. Acetylation by NAT8 and NAT8B is transient and deacetylation probably occurs in the Golgi. Acetylation regulates the maturation, the transport to the plasma membrane, the stability and the expression of the protein.

Palmitoylation mediates lipid raft localization.

Ubiquitinated at Lys-501, ubiquitination leads to lysosomal degradation. Monoubiquitinated and 'Lys-63'-linked polyubitinated. Deubiquitnated by USP8; inhibits lysosomal degradation.

Phosphorylation at Ser-498 is required for interaction with GGA1 and retrograded transport from endosomal compartments to the trans-Golgi network. Non-phosphorylated BACE1 enters a direct recycling route to the cell surface.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Golgi apparatus>trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Cytoplasmic vesicle membrane>Single-pass type I membrane protein. Membrane raft. Lysosome. Late endosome. Early endosome. Recycling endosome. Cell projection>Axon. Cell projection>Dendrite.
Note: Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface (PubMed:17425515, PubMed:11466313). Colocalization with APP in early endosomes is due to addition of bisecting N-acetylglucosamine wich blocks targeting to late endosomes and lysosomes (By similarity). Retrogradly transported from endosomal compartments to the trans-Golgi network in a phosphorylation- and GGA1- dependent manner (PubMed:15886016).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.

Subunit Structure:

Monomer. Interacts (via DXXLL motif) with GGA1, GGA2 and GGA3 (via their VHS domain); the interaction highly increases when BACE1 is phosphorylated at Ser-498. Interacts with RTN3 and RTN4. Interacts with SNX6. Interacts with PCSK9. Interacts with NAT8 and NAT8B. Interacts with BIN1. Interacts (via extracellular domain) with ADAM10 (via extracellular domain) (By similarity). Interacts with SORL1; this interaction may affect binding with APP and hence reduce APP cleavage.

Family&Domains:

DXXLL motif is required for a proper endocytosis and retrograde transport to the trans-Golgi network, as well as for regulation of lysosomal degradation.

The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.

Belongs to the peptidase A1 family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

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