MUNC-18a Antibody | Affinity Biosciences

Product:	MUNC-18a Antibody
Catalog:	AF6640
Description:	Rabbit polyclonal antibody to MUNC-18a
Application:	ELISA(peptide)
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	68kD(Calculated).
Uniprot:	P61764
RRID:	AB_2847363

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Secondary Antibodies
Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

MUNC-18a Antibody detects endogenous levels of total MUNC-18a.

RRID:

AB_2847363
Cite Format: Affinity Biosciences Cat# AF6640, RRID:AB_2847363.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ37475; Munc 18 1; Munc 18a; MUNC18 1; N-Sec1; Neuronal SEC1; NSec1; p67; Protein unc-18 homolog 1; Protein unc-18 homolog A; Rb sec1; RBSEC1; STXB1_HUMAN; STXBP1; Syntaxin binding protein 1; Syntaxin-binding protein 1; Unc 18 homolog; Unc 18A; Unc-18A; Unc18 1; UNC18; Unc18-1;

Immunogens

Immunogen:

A synthesized peptide derived from human MUNC-18a.

Uniprot:

P61764(STXB1_HUMAN) >>Visit HPA database.

Gene(ID):

STXBP1(6812)

Expression:

P61764 STXB1_HUMAN:

Brain and spinal cord. Highly enriched in axons.

Sequence:

P61764 STXB1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAPIGLKAVVGEKIMHDVIKKVKKKGEWKVLVVDQLSMRMLSSCCKMTDIMTEGITIVEDINKRREPLPSLEAVYLITPSEKSVHSLISDFKDPPTAKYRAAHVFFTDSCPDALFNELVKSRAAKVIKTLTEINIAFLPYESQVYSLDSADSFQSFYSPHKAQMKNPILERLAEQIATLCATLKEYPAVRYRGEYKDNALLAQLIQDKLDAYKADDPTMGEGPDKARSQLLILDRGFDPSSPVLHELTFQAMSYDLLPIENDVYKYETSGIGEARVKEVLLDEDDDLWIALRHKHIAEVSQEVTRSLKDFSSSKRMNTGEKTTMRDLSQMLKKMPQYQKELSKYSTHLHLAEDCMKHYQGTVDKLCRVEQDLAMGTDAEGEKIKDPMRAIVPILLDANVSTYDKIRIILLYIFLKNGITEENLNKLIQHAQIPPEDSEIITNMAHLGVPIVTDSTLRRRSKPERKERISEQTYQLSRWTPIIKDIMEDTIEDKLDTKHYPYISTRSSASFSTTAVSARYGHWHKNKAPGEYRSGPRLIIFILGGVSLNEMRCAYEVTQANGKWEVLIGSTHILTPQKLLDTLKKLNKTDEEISS

PTMs - P61764 As Substrate

P61764

Site	PTM Type	Enzyme	Source
K7	Ubiquitination		Uniprot
K13	Ubiquitination		Uniprot
S70	Phosphorylation		Uniprot
K92	Ubiquitination		Uniprot
Y145	Phosphorylation		Uniprot
K184	Ubiquitination		Uniprot
K196	Ubiquitination		Uniprot
K208	Ubiquitination		Uniprot
K294	Ubiquitination		Uniprot
S306	Phosphorylation		Uniprot
K308	Ubiquitination		Uniprot
S313	Phosphorylation		Uniprot
K314	Ubiquitination		Uniprot
K321	Methylation		Uniprot
K321	Ubiquitination		Uniprot
K332	Ubiquitination		Uniprot
K333	Ubiquitination		Uniprot
Y337	Phosphorylation		Uniprot
K339	Ubiquitination		Uniprot
K364	Ubiquitination		Uniprot
T376	Phosphorylation		Uniprot
K382	Ubiquitination		Uniprot
S454	Phosphorylation		Uniprot
T455	Phosphorylation		Uniprot
Y473	Phosphorylation		Uniprot
T479	Phosphorylation	Q13627 (DYRK1A)	Uniprot
T489	Phosphorylation		Uniprot
S507	Phosphorylation		Uniprot
S509	Phosphorylation		Uniprot
S516	Phosphorylation		Uniprot
Y531	Phosphorylation		Uniprot
T574	Phosphorylation		Uniprot
K587	Ubiquitination		Uniprot
S593	Phosphorylation		Uniprot
S594	Phosphorylation		Uniprot

Research Backgrounds

P61764_STXB1_HUMAN

Function:

Participates in the regulation of synaptic vesicle docking and fusion through interaction with GTP-binding proteins (By similarity). Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.

Subcellular Location:

Cytoplasm>Cytosol. Membrane>Peripheral membrane protein.

Tissue Specificity:

Brain and spinal cord. Highly enriched in axons.

Subunit Structure:

Interacts with SYTL4 (By similarity). Interacts with STX1A. Interacts with alpha-synuclein/SNCA; this interaction controls SNCA self-replicating aggregation. Interacts with RAB3A; this interaction promotes RAB3A dissociation from the vesicle membrane (By similarity). Interacts with CABP5 (By similarity).

Family&Domains:

Belongs to the STXBP/unc-18/SEC1 family.

Research Fields

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

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MUNC-18a Antibody - #AF6640