RON Antibody - #AF6620
| Product: | RON Antibody |
| Catalog: | AF6620 |
| Description: | Rabbit polyclonal antibody to RON |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Mol.Wt.: | 152kD(Calculated). |
| Uniprot: | Q04912 |
| RRID: | AB_2847343 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF6620, RRID:AB_2847343.
Fold/Unfold
c met related tyrosine kinase; CD136; CD136 antigen; CDw136; Macrophage stimulating 1 receptor (c met related tyrosine kinase); Macrophage stimulating 1 receptor; Macrophage stimulating protein receptor alpha chain; MACROPHAGE STIMULATING PROTEIN RECEPTOR; Macrophage stimulating protein receptor beta chain; Macrophage-Stimulating 1 Receptor (MST1R); Macrophage-stimulating protein receptor beta chain; MSP receptor; Mst1r; MST1R variant RON30; MST1R variant RON62; NPCA3; p185 RON; p185-Ron; Protein-tyrosine kinase 8; PTK 8; ptk8; PTK8 protein tyrosine kinase 8; Recepteur d’origine nantais (RON); RON; RON protein tyrosine kinase; RON variant E2E3; RON_HUMAN; Soluble RON variant 1; Soluble RON variant 2; Soluble RON variant 3; Soluble RON variant 4; Stem cell derived tyrosine kinase;
Immunogens
A synthesized peptide derived from human RON.
- Q04912 RON_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELLPPLPQSFLLLLLLPAKPAAGEDWQCPRTPYAASRDFDVKYVVPSFSAGGLVQAMVTYEGDRNESAVFVAIRNRLHVLGPDLKSVQSLATGPAGDPGCQTCAACGPGPHGPPGDTDTKVLVLDPALPALVSCGSSLQGRCFLHDLEPQGTAVHLAAPACLFSAHHNRPDDCPDCVASPLGTRVTVVEQGQASYFYVASSLDAAVAASFSPRSVSIRRLKADASGFAPGFVALSVLPKHLVSYSIEYVHSFHTGAFVYFLTVQPASVTDDPSALHTRLARLSATEPELGDYRELVLDCRFAPKRRRRGAPEGGQPYPVLRVAHSAPVGAQLATELSIAEGQEVLFGVFVTGKDGGPGVGPNSVVCAFPIDLLDTLIDEGVERCCESPVHPGLRRGLDFFQSPSFCPNPPGLEALSPNTSCRHFPLLVSSSFSRVDLFNGLLGPVQVTALYVTRLDNVTVAHMGTMDGRILQVELVRSLNYLLYVSNFSLGDSGQPVQRDVSRLGDHLLFASGDQVFQVPIQGPGCRHFLTCGRCLRAWHFMGCGWCGNMCGQQKECPGSWQQDHCPPKLTEFHPHSGPLRGSTRLTLCGSNFYLHPSGLVPEGTHQVTVGQSPCRPLPKDSSKLRPVPRKDFVEEFECELEPLGTQAVGPTNVSLTVTNMPPGKHFRVDGTSVLRGFSFMEPVLIAVQPLFGPRAGGTCLTLEGQSLSVGTSRAVLVNGTECLLARVSEGQLLCATPPGATVASVPLSLQVGGAQVPGSWTFQYREDPVVLSISPNCGYINSHITICGQHLTSAWHLVLSFHDGLRAVESRCERQLPEQQLCRLPEYVVRDPQGWVAGNLSARGDGAAGFTLPGFRFLPPPHPPSANLVPLKPEEHAIKFEYIGLGAVADCVGINVTVGGESCQHEFRGDMVVCPLPPSLQLGQDGAPLQVCVDGECHILGRVVRPGPDGVPQSTLLGILLPLLLLVAALATALVFSYWWRRKQLVLPPNLNDLASLDQTAGATPLPILYSGSDYRSGLALPAIDGLDSTTCVHGASFSDSEDESCVPLLRKESIQLRDLDSALLAEVKDVLIPHERVVTHSDRVIGKGHFGVVYHGEYIDQAQNRIQCAIKSLSRITEMQQVEAFLREGLLMRGLNHPNVLALIGIMLPPEGLPHVLLPYMCHGDLLQFIRSPQRNPTVKDLISFGLQVARGMEYLAEQKFVHRDLAARNCMLDESFTVKVADFGLARDILDREYYSVQQHRHARLPVKWMALESLQTYRFTTKSDVWSFGVLLWELLTRGAPPYRHIDPFDLTHFLAQGRRLPQPEYCPDSLYQVMQQCWEADPAVRPTFRVLVGEVEQIVSALLGDHYVQLPATYMNLGPSTSHEMNVRPEQPQFSPMPGNVRRPRPLSEPPRPT
PTMs - Q04912 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S215 | Phosphorylation | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| N488 | N-Glycosylation | Uniprot | |
| Y1012 | Phosphorylation | Uniprot | |
| Y1017 | Phosphorylation | Uniprot | |
| S1056 | Phosphorylation | Uniprot | |
| K1071 | Ubiquitination | Uniprot | |
| Y1101 | Phosphorylation | Uniprot | |
| Y1198 | Phosphorylation | Uniprot | |
| K1223 | Ubiquitination | Uniprot | |
| Y1238 | Phosphorylation | Q04912 (MST1R) , K9JYP4 (RON) , P08581 (MET) | Uniprot |
| Y1239 | Phosphorylation | P08581 (MET) , K9JYP4 (RON) , Q04912 (MST1R) | Uniprot |
| S1240 | Phosphorylation | Uniprot | |
| Y1317 | Phosphorylation | Uniprot | |
| Y1353 | Phosphorylation | Q04912 (MST1R) , P08581 (MET) , K9JYP4 (RON) | Uniprot |
| Y1360 | Phosphorylation | P08581 (MET) , K9JYP4 (RON) , Q04912 (MST1R) | Uniprot |
| S1394 | Phosphorylation | P31749 (AKT1) | Uniprot |
PTMs - Q04912 As Enzyme
Research Backgrounds
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Plays also a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.
Proteolytic processing yields the two subunits.
Autophosphorylated in response to ligand binding on Tyr-1238 and Tyr-1239 in the kinase domain leading to further phosphorylation of Tyr-1353 and Tyr-1360 in the C-terminal multifunctional docking site.
Ubiquitinated. Ubiquitination by CBL regulates the receptor stability and activity through proteasomal degradation.
Membrane>Single-pass type I membrane protein.
Expressed in colon, skin, lung and bone marrow.
Heterodimer of an alpha chain and a beta chain which are disulfide linked. Binds PLXNB1. Associates with and is negatively regulated by HYAL2. Interacts when phosphorylated with downstream effectors including PIK3R1, PCLG1, GRB2 and GAB1. Interacts with integrin beta1/ITGB1 in a ligand-independent fashion.
Belongs to the protein kinase superfamily. Tyr protein kinase family.
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