EEF1A2 Antibody | Affinity Biosciences

Product:	EEF1A2 Antibody
Catalog:	AF6618
Description:	Rabbit polyclonal antibody to EEF1A2
Application:	ELISA(peptide)
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	50kD(Calculated).
Uniprot:	Q05639
RRID:	AB_2847341

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Secondary Antibodies
Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

EEF1A2 Antibody detects endogenous levels of total EEF1A2.

RRID:

AB_2847341
Cite Format: Affinity Biosciences Cat# AF6618, RRID:AB_2847341.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

eEF1A-2; Eef1a2; EEF1AL; EF 1 alpha 2; EF-1-alpha-2; EF1A; EF1A2_HUMAN; Elongation factor 1 A 2; Elongation factor 1 alpha; Elongation factor 1 alpha 2; Elongation factor 1-alpha 2; Eukaryotic elongation factor 1 A-2; Eukaryotic translation elongation factor 1 alpha 2; FLJ41696; HS1; OTTHUMP00000031776; Statin like; Statin S1; Statin-S1; STN; STNL;

Immunogens

Immunogen:

A synthesized peptide derived from human EEF1A2.

Uniprot:

Q05639(EF1A2_HUMAN) >>Visit HPA database.

Gene(ID):

EEF1A2(1917)

Expression:

Q05639 EF1A2_HUMAN:

Brain, heart, and skeletal muscle.

Sequence:

Q05639 EF1A2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETTKYYITIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPAYSEKRYDEIVKEVSAYIKKIGYNPATVPFVPISGWHGDNMLEPSPNMPWFKGWKVERKEGNASGVSLLEALDTILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGILRPGMVVTFAPVNITTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDIRRGNVCGDSKSDPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACKFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKKSGGAGKVTKSAQKAQKAGK

PTMs - Q05639 As Substrate

Q05639

Site	PTM Type	Enzyme	Source
S21	Phosphorylation	P15056 (BRAF) , P04049 (RAF1)	Uniprot
T22	Phosphorylation		Uniprot
T23	Phosphorylation		Uniprot
T24	Phosphorylation		Uniprot
Y29	Phosphorylation		Uniprot
K30	Acetylation		Uniprot
K30	Methylation		Uniprot
K30	Ubiquitination		Uniprot
K36	Acetylation		Uniprot
K36	Methylation		Uniprot
K36	Ubiquitination		Uniprot
R37	Methylation		Uniprot
K41	Acetylation		Uniprot
K41	Sumoylation		Uniprot
K41	Ubiquitination		Uniprot
K44	Acetylation		Uniprot
K44	Ubiquitination		Uniprot
K51	Methylation		Uniprot
K51	Ubiquitination		Uniprot
S53	Phosphorylation	P05771 (PRKCB)	Uniprot
K55	Acetylation		Uniprot
K55	Methylation		Uniprot
K55	Sumoylation		Uniprot
K55	Ubiquitination		Uniprot
Y56	Phosphorylation		Uniprot
K62	Methylation		Uniprot
K62	Ubiquitination		Uniprot
R69	Methylation		Uniprot
K79	Acetylation		Uniprot
K79	Methylation		Uniprot
K84	Methylation		Uniprot
Y85	Phosphorylation		Uniprot
Y86	Phosphorylation		Uniprot
T88	Phosphorylation		Uniprot
T104	Phosphorylation		Uniprot
T106	Phosphorylation		Uniprot
S128	Phosphorylation		Uniprot
K129	Ubiquitination		Uniprot
T133	Phosphorylation		Uniprot
R134	Methylation		Uniprot
Y141	Phosphorylation		Uniprot
T142	Phosphorylation		Uniprot
K146	Acetylation		Uniprot
K146	Ubiquitination		Uniprot
K154	Ubiquitination		Uniprot
S163	Phosphorylation		Uniprot
K165	Acetylation		Uniprot
K165	Methylation		Uniprot
K165	Ubiquitination		Uniprot
R166	Methylation		Uniprot
K172	Ubiquitination		Uniprot
S175	Phosphorylation		Uniprot
Y177	Phosphorylation		Uniprot
K179	Acetylation		Uniprot
S205	Phosphorylation	P45983 (MAPK8)	Uniprot
K219	Ubiquitination		Uniprot
T234	Phosphorylation		Uniprot
T239	Phosphorylation		Uniprot
K244	Ubiquitination		Uniprot
Y254	Phosphorylation		Uniprot
K255	Acetylation		Uniprot
K255	Methylation		Uniprot
K255	Ubiquitination		Uniprot
T261	Phosphorylation		Uniprot
R266	Methylation		Uniprot
T269	Phosphorylation		Uniprot
T287	Phosphorylation		Uniprot
S291	Phosphorylation		Uniprot
S300	Phosphorylation		Uniprot
K313	Sumoylation		Uniprot
K313	Ubiquitination		Uniprot
K318	Acetylation		Uniprot
K318	Methylation		Uniprot
K318	Ubiquitination		Uniprot
S358	Phosphorylation	P45983 (MAPK8)	Uniprot
S383	Phosphorylation		Uniprot
K392	Ubiquitination		Uniprot
S393	Phosphorylation		Uniprot
R430	Methylation		Uniprot
T432	Phosphorylation		Uniprot
K439	Acetylation		Uniprot
S445	Phosphorylation		Uniprot
T452	Phosphorylation		Uniprot
K453	Ubiquitination		Uniprot

Research Backgrounds

Q05639_EF1A2_HUMAN

Function:

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

PTMs:

Trimethylated at Lys-165 by EEF1AKMT3. Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4; trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs. Trimethylated at the N-terminus by EEF1AKNMT. Mono- and dimethylated at Lys-55 by EEF1AKNMT; dimethylated form is predominant.

Subcellular Location:

Nucleus.

Tissue Specificity:

Brain, heart, and skeletal muscle.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

EEF1A2 Antibody - #AF6618