TGF alpha Antibody - #AF0262

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Product: TGF alpha Antibody
Catalog: AF0262
Description: Rabbit polyclonal antibody to TGF alpha
Application: WB IHC IF/ICC
Cited expt.: IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse
Mol.Wt.: 10kDa; 17kD(Calculated).
Uniprot: P01135
RRID: AB_2833436

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%)
Clonality:
Polyclonal
Specificity:
TGF alpha Antibody detects endogenous levels of total TGF alpha.
RRID:
AB_2833436
Cite Format: Affinity Biosciences Cat# AF0262, RRID:AB_2833436.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EGF like TGF; EGF-like TGF; ETGF; TFGA; TGF A; TGF type 1; TGF-alpha; Tgfa; TGFA_HUMAN; Transforming growth factor alpha; Transforming growth factor alpha precursor; Wa1; Waved 1;

Immunogens

Immunogen:

A synthesized peptide derived from human TGF alpha, corresponding to a region within C-terminal amino acids.

Uniprot:

P01135(TGFA_HUMAN) >>Visit HPA database.

Gene(ID):
TGFA(7039)
Expression:
P01135 TGFA_HUMAN:

Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines.

Description:
TGFalpha TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Sequence:
MVPSAGQLALFALGIVLAACQALENSTSPLSADPPVAAAVVSHFNDCPDSHTQFCFHGTCRFLVQEDKPACVCHSGYVGARCEHADLLAVVAASQKKQAITALVVVSIVALAVLIITCVLIHCCQVRKHCEWCRALICRHEKPSALLKGRTACCHSETVV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Xenopus
70
Sheep
0
Dog
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

Subcellular Location:

Secreted>Extracellular space.

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

References

1). Germacrone ameliorates acute lung injury induced by intestinal ischemia-reperfusion by regulating macrophage M1 polarization and mitochondrial defects. Acta biochimica et biophysica Sinica, 2024 (PubMed: 39439416) [IF=3.3]

Application: IF/ICC    Species: Mouse    Sample: lung tissues

Figure 2 . Germacrone attenuated the release of inflammatory factors and inhibited apoptosis (A‒D) The mRNA expression levels of IL-1α (A), IL-6 (B), COX2 (C), and TGF-α (D) in each group were determined by qPCR. (E,F) Immunofluorescence staining of ICAM1 (E) and TGF-α (F) in lung tissues from each group. Scale bar: 20 μm. (G) TUNEL staining showing the degree of apoptosis. Scale bar: 20 μm. (H) IHC staining of CD86, CD163, and Gr-1 in lung tissues from each group. Scale bar: 20 μm. (I) Protein expression levels of IL-1β, NOS2, TLR2, CD86, CD115, CD206, ARG1, and CD163 in each group, as detected by western blot analysis. Sham: control group; I/RI: mice whose superior mesenteric artery was completely clamped; I/RI + Ger: I/RI group administered with germacrone. Data are shown as the mean ± SD. *P 
2). Germacrone Ameliorates Acute Lung Injury Induced by Intestinal Ischemia Reperfusion Via Regulating Macrophage M1 Polarization and Mitochondrial Defects. Acta biochimica et biophysica Sinica, 2024 (PubMed: 39439416) [IF=3.3]

Application: IF/ICC    Species: Mouse    Sample: lung tissues

Figure2 Germacrone attenuated the release of inflammatory factors and inhibits apoptosis. (AD) The mRNA expression levels of IL-1α (A), IL-6 (B), COX2 (C) and TGF-α (D) were determined by RT-qPCR in each group, respectively. (E-F) Immunofluorescence staining of ICAM1 (E) and TGF-α (F) in lung tissues of each group(400x), respectively. (G) TUNEL staining was employed to detect apoptosis level (400x). (H) IHC staining of CD86, CD163 and Gr-1 in lung tissues of each group. (I) The protein expression levels of IL-1β, NOS2, TLR2, CD86, CD115, CD206, ARG1, and CD163 were detected by Western blot in each group, respectively. Sham: control group, I/R+Con: chloral hydrate treated group, I/R+Ger: chloral hydrate treated group with Germacone administration. Data were shown as the mean ± SD.

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