c-Myb Antibody - #AF6136
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| Product: | c-Myb Antibody |
| Catalog: | AF6136 |
| Description: | Rabbit polyclonal antibody to c-Myb |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 80kDa; 72kD(Calculated). |
| Uniprot: | P10242 |
| RRID: | AB_2835019 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF6136, RRID:AB_2835019.
Fold/Unfold
C myb; C myb protein; Cmyb; MYB; Myb proto oncogene; V myb myeloblastosis viral oncogene homolog;
Immunogens
- P10242 MYB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MARRPRHSIYSSDEDDEDFEMCDHDYDGLLPKSGKRHLGKTRWTREEDEKLKKLVEQNGTDDWKVIANYLPNRTDVQCQHRWQKVLNPELIKGPWTKEEDQRVIELVQKYGPKRWSVIAKHLKGRIGKQCRERWHNHLNPEVKKTSWTEEEDRIIYQAHKRLGNRWAEIAKLLPGRTDNAIKNHWNSTMRRKVEQEGYLQESSKASQPAVATSFQKNSHLMGFAQAPPTAQLPATGQPTVNNDYSYYHISEAQNVSSHVPYPVALHVNIVNVPQPAAAAIQRHYNDEDPEKEKRIKELELLLMSTENELKGQQVLPTQNHTCSYPGWHSTTIADHTRPHGDSAPVSCLGEHHSTPSLPADPGSLPEESASPARCMIVHQGTILDNVKNLLEFAETLQFIDSFLNTSSNHENSDLEMPSLTSTPLIGHKLTVTTPFHRDQTVKTQKENTVFRTPAIKRSILESSPRTPTPFKHALAAQEIKYGPLKMLPQTPSHLVEDLQDVIKQESDESGIVAEFQENGPPLLKKIKQEVESPTDKSGNFFCSHHWEGDSLNTQLFTQTSPVADAPNILTSSVLMAPASEDEDNVLKAFTVPKNRSLASPLQPCSSTWEPASCGKMEEQMTSSSQARKYVNAFSARTLVM
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P10242 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S11 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S12 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K53 | Ubiquitination | Uniprot | |
| Y69 | Phosphorylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| S116 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K120 | Ubiquitination | Uniprot | |
| C130 | S-Nitrosylation | Uniprot | |
| K143 | Sumoylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| T420 | Phosphorylation | Uniprot | |
| T433 | Phosphorylation | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| K445 | Acetylation | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| T452 | Phosphorylation | Uniprot | |
| R457 | Methylation | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| R465 | Methylation | Uniprot | |
| T466 | Phosphorylation | Uniprot | |
| T468 | Phosphorylation | Uniprot | |
| K471 | Acetylation | Uniprot | |
| K471 | Methylation | Uniprot | |
| K471 | Ubiquitination | Uniprot | |
| K480 | Acetylation | Uniprot | |
| K480 | Sumoylation | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| K485 | Acetylation | Uniprot | |
| K485 | Ubiquitination | Uniprot | |
| K503 | Sumoylation | Uniprot | |
| K527 | Sumoylation | Uniprot | |
| S532 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| T534 | Phosphorylation | Uniprot | |
| K593 | Ubiquitination | Uniprot | |
| S599 | Phosphorylation | Uniprot | |
| K615 | Ubiquitination | Uniprot | |
| T621 | Phosphorylation | Uniprot | |
| S624 | Phosphorylation | Uniprot | |
| K628 | Ubiquitination | Uniprot |
Research Backgrounds
Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
Phosphorylated by HIPK1. This phosphorylation reduces MYB transcription factor activity but not MYB protein levels.
Nucleus.
Binds MYBBP1A. Interacts with HIPK2, MAF and NLK (By similarity). Binds to HIPK1.
Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
Research Fields
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
References
Application: WB Species: human Sample: Gastric cancer cells
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