SIRT6 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from 293 cells(heat shock treatment), using SIRT6 Antibody.

Product:	SIRT6 Antibody
Catalog:	AF6570
Description:	Rabbit polyclonal antibody to SIRT6
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	39kD(Calculated).
Uniprot:	Q8N6T7
RRID:	AB_2847294

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

SIRT6 Antibody detects endogenous levels of total SIRT6.

RRID:

AB_2847294
Cite Format: Affinity Biosciences Cat# AF6570, RRID:AB_2847294.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2810449N18Rik; AI043036; Mono ADP ribosyltransferase sirtuin 6; NAD-dependent protein deacetylase sirtuin-6; Regulatory protein SIR2 homolog 6; Regulatory protein SIR2 homolog; SIR2 like 6; SIR2 like protein 6; Sir2 related protein type 6; SIR2-like protein 6; SIR2L6; SIR6_HUMAN; SIRT 6; SIRT6; Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae); Sirtuin 6; Sirtuin type 6; Sirtuin6;

Immunogens

Immunogen:

A synthesized peptide derived from human SIRT6.

Uniprot:

Q8N6T7(SIR6_HUMAN) >>Visit HPA database.

Gene(ID):

SIRT6(51548)

Sequence:

Q8N6T7 SIR6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSVNYAAGLSPYADKGKCGLPEIFDPPEELERKVWELARLVWQSSSVVFHTGAGISTASGIPDFRGPHGVWTMEERGLAPKFDTTFESARPTQTHMALVQLERVGLLRFLVSQNVDGLHVRSGFPRDKLAELHGNMFVEECAKCKTQYVRDTVVGTMGLKATGRLCTVAKARGLRACRGELRDTILDWEDSLPDRDLALADEASRNADLSITLGTSLQIRPSGNLPLATKRRGGRLVIVNLQPTKHDRHADLRIHGYVDEVMTRLMKHLGLEIPAWDGPRVLERALPPLPRPPTPKLEPKEESPTRINGSIPAGPKQEPCAQHNGSEPASPKRERPTSPAPHRPPKRVKAKAVPS

PTMs - Q8N6T7 As Substrate

Q8N6T7

Site	PTM Type	Enzyme	Source
S2	Acetylation		Uniprot
S10	Phosphorylation		Uniprot
K17	Ubiquitination		Uniprot
K81	Ubiquitination		Uniprot
K128	Ubiquitination		Uniprot
T146	Phosphorylation		Uniprot
Y148	Phosphorylation		Uniprot
K160	Ubiquitination		Uniprot
T162	Phosphorylation		Uniprot
K170	Ubiquitination		Uniprot
K245	Ubiquitination		Uniprot
K267	Ubiquitination		Uniprot
T294	Phosphorylation		Uniprot
S303	Phosphorylation		Uniprot
T305	Phosphorylation		Uniprot
S310	Phosphorylation		Uniprot
S326	Phosphorylation		Uniprot
S330	Phosphorylation		Uniprot
T337	Phosphorylation		Uniprot
S338	Phosphorylation	P31749 (AKT1)	Uniprot

Research Backgrounds

Q8N6T7_SIR6_HUMAN

Function:

NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.

Subcellular Location:

Nucleus>Nucleoplasm.
Note: Predominantly nuclear. Associated with telomeric heterochromatin regions.

Subunit Structure:

Interacts with RELA. Interacts with RBBP8; the interaction deacetylates RBBP8.

Family&Domains:

Belongs to the sirtuin family. Class IV subfamily.

Research Fields

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)

References

1). Rosmarinic Acid Activates the Nrf2/ARE Signaling Pathway via the miR-25-3p/SIRT6 Axis to Inhibit Vascular Remodeling. Journal of agricultural and food chemistry, 2024 (PubMed: 38373191) [IF=6.1]

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SIRT6 Antibody - #AF6570