Nanog Antibody | Affinity Biosciences

Product:	Nanog Antibody
Catalog:	AF6535
Description:	Rabbit polyclonal antibody to Nanog
Application:	ELISA(peptide)
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	35kD(Calculated).
Uniprot:	Q9H9S0
RRID:	AB_2847259

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Secondary Antibodies
Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Nanog Antibody detects endogenous levels of total Nanog.

RRID:

AB_2847259
Cite Format: Affinity Biosciences Cat# AF6535, RRID:AB_2847259.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Embryonic stem cell specific homeobox protein (Nanog); ENK; FLJ12581; hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; NANOG; Nanog homeobox; NANOG_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human Nanog.

Uniprot:

Q9H9S0(NANOG_HUMAN) >>Visit HPA database.

Gene(ID):

NANOG(79923)

Expression:

Q9H9S0 NANOG_HUMAN:

Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

Sequence:

Q9H9S0 NANOG_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSVDPACPQSLPCFEASDCKESSPMPVICGPEENYPSLQMSSAEMPHTETVSPLPSSMDLLIQDSPDSSTSPKGKQPTSAEKSVAKKEDKVPVKKQKTRTVFSSTQLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRWQKNNWPKNSNGVTQKASAPTYPSLYSSYHQGCLVNPTGNLPMWSNQTWNNSTWSNQTQNIQSWSNHSWNTQTWCTQSWNNQAWNSPFYNCGEESLQSCMQFQPNSPASDLEAALEAAGEGLNVIQQTTRYFSTPQTMDLFLNYSMNMQPEDV

PTMs - Q9H9S0 As Substrate

Q9H9S0

Site	PTM Type	Enzyme	Source
S2	Phosphorylation		Uniprot
Y35	Phosphorylation	Q05397 (PTK2)	Uniprot
T50	Phosphorylation		Uniprot
S52	Phosphorylation	P28482 (MAPK1)	Uniprot
S56	Phosphorylation		Uniprot
S57	Phosphorylation		Uniprot
S65	Phosphorylation		Uniprot
T70	Phosphorylation		Uniprot
S71	Phosphorylation	P28482 (MAPK1) , P06493 (CDK1)	Uniprot
T78	Phosphorylation	Q02156 (PRKCE) , P28482 (MAPK1)	Uniprot
S79	Phosphorylation	Q02156 (PRKCE)	Uniprot
S135	Phosphorylation	Q02156 (PRKCE)	Uniprot
Y174	Phosphorylation	Q05397 (PTK2)	Uniprot
T200	Phosphorylation	Q02156 (PRKCE)	Uniprot
S258	Phosphorylation		Uniprot
T280	Phosphorylation	Q02156 (PRKCE)	Uniprot
T286	Phosphorylation		Uniprot
T289	Phosphorylation		Uniprot

Research Backgrounds

Q9H9S0_NANOG_HUMAN

Function:

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Subcellular Location:

Nucleus.

Tissue Specificity:

Subunit Structure:

Interacts with SMAD1 and SALL4. Interacts with ZNF281/ZFP281 (By similarity). Interacts with PCGF1. Interacts with ESRRB; reciprocally modulates their transcriptional activities (By similarity).

Family&Domains:

Belongs to the Nanog homeobox family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

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For Research Use Only.
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Nanog Antibody - #AF6535