ASF1A Antibody - #AF6510
| Product: | ASF1A Antibody |
| Catalog: | AF6510 |
| Description: | Rabbit polyclonal antibody to ASF1A |
| Application: | ELISA(peptide) |
| Reactivity: | Human, Mouse, Rat |
| Mol.Wt.: | 20~25kD; 23kD(Calculated). |
| Uniprot: | Q9Y294 |
| RRID: | AB_2847234 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF6510, RRID:AB_2847234.
Fold/Unfold
Anti silencing function 1A; Anti-silencing function protein 1 homolog A; ASF1 anti silencing function 1 homolog A (S. cerevisiae); ASF1 anti silencing function 1 homolog A; asf1a; ASF1A_HUMAN; CCG1 interacting factor A; CCG1-interacting factor A; CGI 98; CIA; hAsf1; hAsf1a; hCIA; Histone chaperone ASF1A; HSPC146;
Immunogens
A synthesized peptide derived from human ASF1A.
- Q9Y294 ASF1A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAKVQVNNVVVLDNPSPFYNPFQFEITFECIEDLSEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRHMFVFQADAPNPGLIPDADAVGVTVVLITCTYRGQEFIRVGYYVNNEYTETELRENPPVKPDFSKLQRNILASNPRVTRFHINWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCM
PTMs - Q9Y294 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T120 | Phosphorylation | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| T157 | Phosphorylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| T176 | Phosphorylation | Uniprot | |
| S192 | Phosphorylation | Q86UE8 (TLK2) | Uniprot |
Research Backgrounds
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase. TLK2-mediated phosphorylation at Ser-192 prevents proteasome-dependent degradation.
Nucleus.
Ubiquitously expressed.
Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with CABIN1, HAT1, HIRA, NASP, TAF1, TLK1, TLK2 and UBN1. Interacts with CDAN1. Found in a cytosolic complex with CDAN1, ASF1B, IPO4 and histones H3.1 and H4. Interacts with CREBBP.
Belongs to the ASF1 family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.