Phospho-ZO-2 (Tyr1118) Antibody - #AF3874

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Product: Phospho-ZO-2 (Tyr1118) Antibody
Catalog: AF3874
Description: Rabbit polyclonal antibody to Phospho-ZO-2 (Tyr1118)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 134kD(Calculated).
Uniprot: Q9UDY2
RRID: AB_2847188

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-ZO-2 (Tyr1118) Antibody detects endogenous levels of ZO-2 only when phosphorylated at Tyr1118.
RRID:
AB_2847188
Cite Format: Affinity Biosciences Cat# AF3874, RRID:AB_2847188.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C9DUPq21.11; DFNA51; DUP9q21.11; Friedreich ataxia region gene X104 (tight junction protein ZO-2); MGC26306; PFIC4; Tight junction protein 2; Tight junction protein ZO 2; Tight junction protein ZO-2; TJP2; X104; ZO 2; ZO-2; ZO2; ZO2_HUMAN; Zona occludens 2; Zona occludens protein 2; Zonula occludens protein 2;

Immunogens

Immunogen:

A synthesized peptide derived from human ZO-2 around the phosphorylation site of Tyr1118.

Uniprot:

Q9UDY2(ZO2_HUMAN) >>Visit HPA database.

Gene(ID):
TJP2(9414)
Expression:
Q9UDY2 ZO2_HUMAN:

This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues.

Sequence:
MPVRGDRGFPPRRELSGWLRAPGMEELIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKVAAIVVKRPRKVQVAALQASPPLDQDDRAFEVMDEFDGRSFRSGYSERSRLNSHGGRSRSWEDSPERGRPHERARSRERDLSRDRSRGRSLERGLDQDHARTRDRSRGRSLERGLDHDFGPSRDRDRDRSRGRSIDQDYERAYHRAYDPDYERAYSPEYRRGARHDARSRGPRSRSREHPHSRSPSPEPRGRPGPIGVLLMKSRANEEYGLRLGSQIFVKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSQQTLINIPSLNDSDSEIEDISEIESNRSFSPEERRHQYSDYDYHSSSEKLKERPSSREDTPSRLSRMGATPTPFKSTGDIAGTVVPETNKEPRYQEDPPAPQPKAAPRTFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGEMVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGNWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRDNAGDRADFWRMRGQRSGVKKNLRKSREDLTAVVSVSTKFPAYERVLLREAGFKRPVVLFGPIADIAMEKLANELPDWFQTAKTEPKDAGSEKSTGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTMRQRLNPTSNKSSRKLFDQANKLKKTCAHLFTATINLNSANDSWFGSLKDTIQHQQGEAVWVSEGKMEGMDDDPEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELDEPAEEPLVSSITRSSEPVQHEESIRKPSPEPRAQMRRAASSDQLRDNSPPPAFKPEPPKAKTQNKEESYDFSKSYEYKSNPSAVAGNETPGASTKGYPPPVAAKPTFGRSILKPSTPIPPQEGEEVGESSEEQDNAPKSVLGKVKIFEKMDHKARLQRMQELQEAQNARIEIAQKHPDIYAVPIKTHKPDPGTPQHTSSRPPEPQKAPSRPYQDTRGSYGSDAEEEEYRQQLSEHSKRGYYGQSARYRDTEL

PTMs - Q9UDY2 As Substrate

Site PTM Type Enzyme
S16 Phosphorylation
T91 Phosphorylation
S130 Phosphorylation
S150 Phosphorylation
S153 Phosphorylation
S156 Phosphorylation
S163 Phosphorylation
S168 Phosphorylation
S170 Phosphorylation
S174 Phosphorylation
S186 Phosphorylation
S192 Phosphorylation
S196 Phosphorylation
S200 Phosphorylation
T212 Phosphorylation
S216 Phosphorylation
S220 Phosphorylation
S232 Phosphorylation
S240 Phosphorylation
S244 Phosphorylation
Y249 Phosphorylation
Y253 Phosphorylation
Y257 Phosphorylation
Y261 Phosphorylation
Y265 Phosphorylation
S266 Phosphorylation
Y269 Phosphorylation
S279 Phosphorylation
S284 Phosphorylation
S286 Phosphorylation
S292 Phosphorylation
S294 Phosphorylation
S296 Phosphorylation
Y319 Phosphorylation
S325 Phosphorylation
T333 Phosphorylation
T335 Phosphorylation
T339 Phosphorylation
S385 Phosphorylation
S394 Phosphorylation
S398 Phosphorylation
S400 Phosphorylation
S406 Phosphorylation
S413 Phosphorylation
S415 Phosphorylation
Y423 Phosphorylation
S424 Phosphorylation
Y426 Phosphorylation
Y428 Phosphorylation
S430 Phosphorylation
S431 Phosphorylation
S432 Phosphorylation
S440 Phosphorylation
S441 Phosphorylation
T445 Phosphorylation
S450 Phosphorylation
T455 Phosphorylation
T457 Phosphorylation
K460 Methylation
S461 Phosphorylation
T462 Phosphorylation
T468 Phosphorylation
K475 Ubiquitination
Y479 Phosphorylation
T494 Phosphorylation
S499 Phosphorylation
Y506 Phosphorylation
T510 Phosphorylation
S520 Phosphorylation
Y574 Phosphorylation
T585 Phosphorylation
S590 Phosphorylation
S606 Phosphorylation
Y638 Phosphorylation
S702 Phosphorylation
T707 Phosphorylation
S711 Phosphorylation
S713 Phosphorylation
K730 Methylation
T760 Phosphorylation
S767 Phosphorylation
K769 Ubiquitination
S770 Phosphorylation
T771 Phosphorylation
T778 Phosphorylation
T831 Phosphorylation
S832 Phosphorylation
S836 Phosphorylation
S886 Phosphorylation
S902 Phosphorylation
Y903 Phosphorylation
T905 Phosphorylation
Y911 Phosphorylation
S913 Phosphorylation
S916 Phosphorylation
S920 Phosphorylation
T925 Phosphorylation
Y932 Phosphorylation
T933 Phosphorylation
S953 Phosphorylation
S961 Phosphorylation
S966 Phosphorylation
S978 Phosphorylation
S979 Phosphorylation
S986 Phosphorylation
S1006 Phosphorylation
Y1007 Phosphorylation
S1012 Phosphorylation
Y1013 Phosphorylation
Y1015 Phosphorylation
K1016 Ubiquitination
T1027 Phosphorylation
S1031 Phosphorylation
S1048 Phosphorylation
S1053 Phosphorylation
T1054 Phosphorylation
S1067 Phosphorylation
S1068 Phosphorylation
Y1118 Phosphorylation
T1124 Phosphorylation
T1131 Phosphorylation
T1135 Phosphorylation
S1137 Phosphorylation
S1147 Phosphorylation
Y1150 Phosphorylation
S1156 Phosphorylation
Y1157 Phosphorylation
S1159 Phosphorylation
Y1166 Phosphorylation
S1171 Phosphorylation
S1174 Phosphorylation
R1176 Methylation
Y1178 Phosphorylation
Y1179 Phosphorylation
S1182 Phosphorylation

Research Backgrounds

Function:

Plays a role in tight junctions and adherens junctions.

Subcellular Location:

Cell junction>Adherens junction. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Tight junction. Nucleus.
Note: Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues.

Subunit Structure:

Homodimer, and heterodimer with ZO1. Interacts with occludin, SAFB and UBN1. Interaction with SAFB occurs in the nucleus. Interacts with SCRIB. Interacts with USP53 (via the C-terminal region) (By similarity).

Family&Domains:

Belongs to the MAGUK family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

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