Phospho-MLC2 (Tyr118) Antibody - #AF3869
| Product: | Phospho-MLC2 (Tyr118) Antibody |
| Catalog: | AF3869 |
| Description: | Rabbit polyclonal antibody to Phospho-MLC2 (Tyr118) |
| Application: | IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Mol.Wt.: | 19kD(Calculated). |
| Uniprot: | P10916 |
| RRID: | AB_2847183 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF3869, RRID:AB_2847183.
Fold/Unfold
Cardiac myosin light chain-2; Cardiac ventricular myosin light chain 2; CMH10; MLC 2v; MLC-2; MLC-2v; MLC2; MLRV_HUMAN; MYL 2; MYL2; Myosin light chain 2 regulatory cardiac slow; Myosin light polypeptide 2 regulatory cardiac slow; Myosin regulatory light chain 2; Myosin regulatory light chain 2 ventricular/cardiac muscle isoform; Regulatory light chain of myosin; RLC of myosin; Slow cardiac myosin regulatory light chain 2; ventricular/cardiac muscle isoform;
Immunogens
A synthesized peptide derived from human MLC2 around the phosphorylation site of Tyr118.
- P10916 MLRV_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPKKAKKRAGGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRDTFAALGRVNVKNEEIDEMIKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGKGVLKADYVREMLTTQAERFSKEEVDQMFAAFPPDVTGNLDYKNLVHIITHGEEKD
PTMs - P10916 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S15 | Phosphorylation | Q15746 (MYLK) | Uniprot |
| S19 | Phosphorylation | Q5VT25 (CDC42BPA) | Uniprot |
| T80 | Phosphorylation | Uniprot | |
| T98 | Phosphorylation | Uniprot | |
| K104 | Acetylation | Uniprot | |
| K111 | Acetylation | Uniprot | |
| Y118 | Phosphorylation | Uniprot | |
| T124 | Phosphorylation | Uniprot | |
| Y152 | Phosphorylation | Uniprot | |
| K153 | Acetylation | Uniprot | |
| K165 | Acetylation | Uniprot |
Research Backgrounds
Contractile protein that plays a role in heart development and function (By similarity). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).
N-terminus is methylated by METTL11A/NTM1.
Phosphorylated by MYLK3 and MYLK2; promotes cardiac muscle contraction and function (By similarity). Dephosphorylated by PPP1CB complexed to PPP1R12B (By similarity). The phosphorylated form in adult is expressed as gradients across the heart from endocardium (low phosphorylation) to epicardium (high phosphorylation); regulates cardiac torsion and workload distribution (By similarity).
Cytoplasm>Myofibril>Sarcomere>A band.
Myosin is a hexamer of 2 heavy chains and 4 light chains. Interacts with MYOC.
Research Fields
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).
· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).
· Organismal Systems > Circulatory system > Cardiac muscle contraction. (View pathway)
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
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