Phospho-HMGCR (Ser872) Antibody | Affinity Biosciences

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Western blot analysis of extracts from Mouse brain, using Phospho-HMGCR (Ser872) Antibody.

Product:	Phospho-HMGCR (Ser872) Antibody
Catalog:	AF3865
Description:	Rabbit polyclonal antibody to Phospho-HMGCR (Ser872)
Application:	WB
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	97kD(Calculated).
Uniprot:	P04035
RRID:	AB_2847179

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-HMGCR (Ser872) Antibody detects endogenous levels of HMGCR only when phosphorylated at Ser872.

RRID:

AB_2847179
Cite Format: Affinity Biosciences Cat# AF3865, RRID:AB_2847179.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

3 hydroxy 3 methylglutaryl CoA reductase; 3 hydroxy 3 methylglutaryl Coenzyme A reductase; 3 hydroxymethylglutaryl CoA reductase; 3-hydroxy-3-methylglutaryl CoA reductase (NADPH); 3-hydroxy-3-methylglutaryl-coenzyme A reductase; 3H3M; HMDH_HUMAN; HMG CoA reductase; HMG CoAR; HMG-CoA reductase; Hmgcr; Hydroxymethylglutaryl CoA reductase; LDLCQ3; MGC103269; Red;

Immunogens

Immunogen:

A synthesized peptide derived from human HMGCR around the phosphorylation site of Ser872.

Uniprot:

P04035(HMDH_HUMAN) >>Visit HPA database.

Gene(ID):

HMGCR(3156)

Sequence:

P04035 HMDH_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLSRLFRMHGLFVASHPWEVIVGTVTLTICMMSMNMFTGNNKICGWNYECPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSRASTLAKFALSSNSQDEVRENIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFVFMTFFPACVSLVLELSRESREGRPIWQLSHFARVLEEEENKPNPVTQRVKMIMSLGLVLVHAHSRWIADPSPQNSTADTSKVSLGLDENVSKRIEPSVSLWQFYLSKMISMDIEQVITLSLALLLAVKYIFFEQTETESTLSLKNPITSPVVTQKKVPDNCCRREPMLVRNNQKCDSVEEETGINRERKVEVIKPLVAETDTPNRATFVVGNSSLLDTSSVLVTQEPEIELPREPRPNEECLQILGNAEKGAKFLSDAEIIQLVNAKHIPAYKLETLMETHERGVSIRRQLLSKKLSEPSSLQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPLCLDEKEFQVPMATTEGCLVASTNRGCRAIGLGGGASSRVLADGMTRGPVVRLPRACDSAEVKAWLETSEGFAVIKEAFDSTSRFARLQKLHTSIAGRNLYIRFQSRSGDAMGMNMISKGTEKALSKLHEYFPEMQILAVSGNYCTDKKPAAINWIEGRGKSVVCEAVIPAKVVREVLKTTTEAMIEVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAALAAGHLVKSHMIHNRSKINLQDLQGACTKKTA

PTMs - P04035 As Substrate

P04035

Site	PTM Type	Source
K89	Ubiquitination	Uniprot
K142	Ubiquitination	Uniprot
K248	Ubiquitination	Uniprot
S261	Phosphorylation	Uniprot
S278	Phosphorylation	Uniprot
T286	Phosphorylation	Uniprot
K288	Ubiquitination	Uniprot
K299	Ubiquitination	Uniprot
K351	Ubiquitination	Uniprot
S356	Phosphorylation	Uniprot
K362	Ubiquitination	Uniprot
K363	Ubiquitination	Uniprot
K381	Ubiquitination	Uniprot
T389	Phosphorylation	Uniprot
K396	Ubiquitination	Uniprot
K401	Ubiquitination	Uniprot
T409	Phosphorylation	Uniprot
S420	Phosphorylation	Uniprot
T425	Phosphorylation	Uniprot
S427	Phosphorylation	Uniprot
T431	Phosphorylation	Uniprot
K457	Ubiquitination	Uniprot
K460	Ubiquitination	Uniprot
S463	Phosphorylation	Uniprot
K474	Ubiquitination	Uniprot
K480	Ubiquitination	Uniprot
K502	Ubiquitination	Uniprot
S504	Phosphorylation	Uniprot
S602	Phosphorylation	Uniprot
K606	Ubiquitination	Uniprot
K619	Ubiquitination	Uniprot
K633	Ubiquitination	Uniprot
S637	Phosphorylation	Uniprot
Y644	Phosphorylation	Uniprot
K662	Ubiquitination	Uniprot
K666	Ubiquitination	Uniprot
K670	Ubiquitination	Uniprot
K692	Sumoylation	Uniprot
K692	Ubiquitination	Uniprot
K704	Ubiquitination	Uniprot
K715	Ubiquitination	Uniprot
K864	Ubiquitination	Uniprot
S872	Phosphorylation	Uniprot
K873	Ubiquitination	Uniprot
T884	Phosphorylation	Uniprot
K885	Ubiquitination	Uniprot

Research Backgrounds

P04035_HMDH_HUMAN

Function:

Transmembrane glycoprotein that is the rate-limiting enzyme in cholesterol biosynthesis as well as in the biosynthesis of nonsterol isoprenoids that are essential for normal cell function including ubiquinone and geranylgeranyl proteins.

PTMs:

N-glycosylated. Deglycosylated by NGLY1 on release from the endoplasmic reticulum (ER) in a sterol-mediated manner.

Undergoes sterol-mediated ubiquitination and ER-associated degradation (ERAD). Accumulation of sterols in the endoplasmic reticulum (ER) membrane, triggers binding of the reductase to the ER membrane protein INSIG1. This INSIG1 binding leads to the recruitment of the ubiquitin ligase, AMFR/gp78 or RNF145, initiating ubiquitination of the reductase. The ubiquitinated reductase is then extracted from the ER membrane and delivered to cytosolic 26S proteosomes by a mechanism probably mediated by the ATPase Valosin-containing protein VCP/p97. Lys-248 is the main site of ubiquitination. Ubiquitination is enhanced by the presence of a geranylgeranylated protein.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Subunit Structure:

Homodimer. Interacts (via its SSD) with INSIG1; the interaction, accelerated by sterols, leads to the recruitment of HMGCR to AMFR/gp78 for its ubiquitination by the sterol-mediated ERAD pathway. Interacts with UBIAD1.

Family&Domains:

Belongs to the HMG-CoA reductase family.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Metabolism > Metabolism of terpenoids and polyketides > Terpenoid backbone biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

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Phospho-HMGCR (Ser872) Antibody - #AF3865