Phospho-ZEB2 (Ser784) Antibody - #AF3840

(3)  
Product: Phospho-ZEB2 (Ser784) Antibody
Catalog: AF3840
Description: Rabbit polyclonal antibody to Phospho-ZEB2 (Ser784)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 136kD(Calculated).
Uniprot: O60315
RRID: AB_2847154

View similar products>>

   Size Price Inventory
 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-ZEB2 (Ser784) Antibody detects endogenous levels of ZEB2 only when phosphorylated at Ser784.
RRID:
AB_2847154
Cite Format: Affinity Biosciences Cat# AF3840, RRID:AB_2847154.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ42816; HSPC082; KIAA0569; SIP 1; SIP1; Smad Interacting Protein 1; Smad-interacting protein 1; SMADIP 1; SMADIP1; ZEB 2; Zeb2; ZEB2_HUMAN; Zfhx1b; ZFHX1B protein; Zfx1b; Zinc finger E box binding protein 2; Zinc finger E-box-binding homeobox 2; Zinc finger homeobox 1b; zinc finger homeobox protein 1; Zinc finger homeobox protein 1b;

Immunogens

Immunogen:

A synthesized peptide derived from human ZEB2 around the phosphorylation site of Ser784.

Uniprot:

O60315(ZEB2_HUMAN) >>Visit HPA database.

Gene(ID):
ZEB2(9839)
Sequence:
MKQPIMADGPRCKRRKQANPRRKNVVNYDNVVDTGSETDEEDKLHIAEDDGIANPLDQETSPASVPNHESSPHVSQALLPREEEEDEIREGGVEHPWHNNEILQASVDGPEEMKEDYDTMGPEATIQTAINNGTVKNANCTSDFEEYFAKRKLEERDGHAVSIEEYLQRSDTAIIYPEAPEELSRLGTPEANGQEENDLPPGTPDAFAQLLTCPYCDRGYKRLTSLKEHIKYRHEKNEENFSCPLCSYTFAYRTQLERHMVTHKPGTDQHQMLTQGAGNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGLISVNGRMRNNIKTGSSPNSVSSSPTNSAITQLRNKLENGKPLSMSEQTGLLKIKTEPLDFNDYKVLMATHGFSGTSPFMNGGLGATSPLGVHPSAQSPMQHLGVGMEAPLLGFPTMNSNLSEVQKVLQIVDNTVSRQKMDCKAEEISKLKGYHMKDPCSQPEEQGVTSPNIPPVGLPVVSHNGATKSIIDYTLEKVNEAKACLQSLTTDSRRQISNIKKEKLRTLIDLVTDDKMIENHNISTPFSCQFCKESFPGPIPLHQHERYLCKMNEEIKAVLQPHENIVPNKAGVFVDNKALLLSSVLSEKGMTSPINPYKDHMSVLKAYYAMNMEPNSDELLKISIAVGLPQEFVKEWFEQRKVYQYSNSRSPSLERSSKPLAPNSNPPTKDSLLPRSPVKPMDSITSPSIAELHNSVTNCDPPLRLTKPSHFTNIKPVEKLDHSRSNTPSPLNLSSTSSKNSHSSSYTPNSFSSEELQAEPLDLSLPKQMKEPKSIIATKNKTKASSISLDHNSVSSSSENSDEPLNLTFIKKEFSNSNNLDNKSTNPVFSMNPFSAKPLYTALPPQSAFPPATFMPPVQTSIPGLRPYPGLDQMSFLPHMAYTYPTGAATFADMQQRRKYQRKQGFQGELLDGAQDYMSGLDDMTDSDSCLSRKKIKKTESGMYACDLCDKTFQKSSSLLRHKYEHTGKRPHQCQICKKAFKHKHHLIEHSRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEEREAAEREAREKGHLEPTELLMNRAYLQSITPQGYSDSEERESMPRDGESEKEHEKEGEDGYGKLGRQDGDEEFEEEEEESENKSMDTDPETIRDEEETGDHSMDDSSEDGKMETKSDHEEDNMEDGM

PTMs - O60315 As Substrate

Site PTM Type Enzyme
R11 Methylation
T34 Phosphorylation
S36 Phosphorylation
T38 Phosphorylation
S64 Phosphorylation
T119 Phosphorylation
S142 Phosphorylation
Y147 Phosphorylation
S162 Phosphorylation
S184 Phosphorylation
T188 Phosphorylation
T254 Phosphorylation
T274 Phosphorylation
T285 Phosphorylation
S305 Phosphorylation
S324 Phosphorylation
S327 Phosphorylation
S330 Phosphorylation
S331 Phosphorylation
T350 Phosphorylation
S352 Phosphorylation
S353 Phosphorylation
S356 Phosphorylation
S358 Phosphorylation
S359 Phosphorylation
S360 Phosphorylation
T362 Phosphorylation
K377 Acetylation
K391 Sumoylation
S410 Phosphorylation
S431 Phosphorylation
T470 Phosphorylation
S505 Phosphorylation
K632 Ubiquitination
S641 Phosphorylation
S647 Phosphorylation
S703 Phosphorylation
S705 Phosphorylation
S707 Phosphorylation
S726 Phosphorylation
S731 Phosphorylation
S741 Phosphorylation
S778 Phosphorylation
S780 Phosphorylation
T782 Phosphorylation
S784 Phosphorylation
S796 Phosphorylation
T802 Phosphorylation
S805 Phosphorylation
S819 Phosphorylation
S829 Phosphorylation
T837 Phosphorylation
S840 Phosphorylation
S841 Phosphorylation
S843 Phosphorylation
S848 Phosphorylation
S853 Phosphorylation
K866 Sumoylation
S1050 Phosphorylation
S1115 Phosphorylation
T1117 Phosphorylation
S1122 Phosphorylation
S1124 Phosphorylation
S1129 Phosphorylation
S1136 Phosphorylation
S1167 Phosphorylation
T1185 Phosphorylation
S1189 Phosphorylation
S1193 Phosphorylation
S1194 Phosphorylation
S1203 Phosphorylation

Research Backgrounds

Function:

Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.

PTMs:

Sumoylation on Lys-391 and Lys-866 is promoted by the E3 SUMO-protein ligase CBX4, and impairs interaction with CTBP1 and transcription repression activity.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds activated SMAD1, activated SMAD2 and activated SMAD3; binding with SMAD4 is not detected (By similarity). Interacts with CBX4 and CTBP1.

Family&Domains:

Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.

Research Fields

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.