Product: Phospho-Mitofusin 2 (Ser442) Antibody
Catalog: AF3838
Description: Rabbit polyclonal antibody to Phospho-Mitofusin 2 (Ser442)
Application: IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 86kD(Calculated).
Uniprot: O95140
RRID: AB_2847152

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Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-Mitofusin 2 (Ser442) Antibody detects endogenous levels of Mitofusin 2 only when phosphorylated at Ser442.
RRID:
AB_2847152
Cite Format: Affinity Biosciences Cat# AF3838, RRID:AB_2847152.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CMT2A; CMT2A2; CPRP 1; CPRP1; EC 3.6.5.-; Fzo; HSG; hyperplasia suppressor gene; Hypertension related protein 1; KIAA0214; MARF; MFN 2; Mfn2; MFN2_HUMAN; Mitochondrial assembly regulatory factor; Mitofusin-2; Mitofusin2; Transmembrane GTPase MFN2;

Immunogens

Immunogen:

A synthesized peptide derived from human Mitofusin 2 around the phosphorylation site of Ser442.

Uniprot:
Gene(ID):
Expression:
O95140 MFN2_HUMAN:

Ubiquitous; expressed at low level. Highly expressed in heart and kidney.

Sequence:
MSLLFSRCNSIVTVKKNKRHMAEVNASPLKHFVTAKKKINGIFEQLGAYIQESATFLEDTYRNAELDPVTTEEQVLDVKGYLSKVRGISEVLARRHMKVAFFGRTSNGKSTVINAMLWDKVLPSGIGHTTNCFLRVEGTDGHEAFLLTEGSEEKRSAKTVNQLAHALHQDKQLHAGSLVSVMWPNSKCPLLKDDLVLMDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKHFFHKVSERLSRPNIFILNNRWDASASEPEYMEEVRRQHMERCTSFLVDELGVVDRSQAGDRIFFVSAKEVLNARIQKAQGMPEGGGALAEGFQVRMFEFQNFERRFEECISQSAVKTKFEQHTVRAKQIAEAVRLIMDSLHMAAREQQVYCEEMREERQDRLKFIDKQLELLAQDYKLRIKQITEEVERQVSTAMAEEIRRLSVLVDDYQMDFHPSPVVLKVYKNELHRHIEEGLGRNMSDRCSTAITNSLQTMQQDMIDGLKPLLPVSVRSQIDMLVPRQCFSLNYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRALMGYNDQVQRPIPLTPANPSMPPLPQGSLTQEEFMVSMVTGLASLTSRTSMGILVVGGVVWKAVGWRLIALSFGLYGLLYVYERLTWTTKAKERAFKRQFVEHASEKLQLVISYTGSNCSHQVQQELSGTFAHLCQQVDVTRENLEQEIAAMNKKIEVLDSLQSKAKLLRNKAGWLDSELNMFTHQYLQPSR

PTMs - O95140 As Substrate

Site PTM Type Enzyme
Ubiquitination
S10 Phosphorylation
S27 Phosphorylation P45984 (MAPK9)
K30 Ubiquitination
K36 Acetylation
K36 Ubiquitination
K79 Ubiquitination
K84 Ubiquitination
K98 Ubiquitination
T111 Phosphorylation
K154 Ubiquitination
K158 Ubiquitination
K171 Ubiquitination
K192 Ubiquitination
K243 Acetylation
K243 Ubiquitination
K307 Ubiquitination
K316 Ubiquitination
K355 Acetylation
K355 Ubiquitination
K357 Ubiquitination
K366 Ubiquitination
K406 Ubiquitination
K416 Ubiquitination
K420 Ubiquitination
T423 Phosphorylation
S442 Phosphorylation
K460 Ubiquitination
K463 Ubiquitination
K560 Ubiquitination
K662 Ubiquitination
K719 Ubiquitination
K720 Ubiquitination
K730 Ubiquitination
K737 Ubiquitination
S756 Phosphorylation

Research Backgrounds

Function:

Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains (Probable). Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes (By similarity). Plays an important role in the regulation of vascular smooth muscle cell proliferation (By similarity). Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PRKN recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress (By similarity). Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions (By similarity).

PTMs:

Phosphorylated by PINK1.

Ubiquitinated by non-degradative ubiquitin by PRKN, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion.

Subcellular Location:

Mitochondrion outer membrane>Multi-pass membrane protein.
Note: Colocalizes with BAX during apoptosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous; expressed at low level. Highly expressed in heart and kidney.

Subunit Structure:

Forms homomultimers and heteromultimers with MFN1. Oligomerization is essential for mitochondrion fusion (Probable). Interacts with VAT1 (By similarity). Interacts with STOML2; may form heterooligomers. Interacts (phosphorylated) with PRKN. Interacts with EIF2AK3 (By similarity).

Family&Domains:

A helix bundle is formed by helices from the N-terminal and the C-terminal part of the protein. The GTPase domain cannot be expressed by itself, without the helix bundle. Rearrangement of the helix bundle and/or of the coiled coil domains may bring membranes from adjacent mitochondria into close contact, and thereby play a role in mitochondrial fusion.

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.

Research Fields

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

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