Phospho-Perilipin-1 (Ser522) Antibody - #AF3834

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Product: Phospho-Perilipin-1 (Ser522) Antibody
Catalog: AF3834
Description: Rabbit polyclonal antibody to Phospho-Perilipin-1 (Ser522)
Application: IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 56kD(Calculated).
Uniprot: O60240
RRID: AB_2847148

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-Perilipin-1 (Ser522) Antibody detects endogenous levels of Perilipin-1 only when phosphorylated at Ser522.
RRID:
AB_2847148
Cite Format: Affinity Biosciences Cat# AF3834, RRID:AB_2847148.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Lipid droplet associated protein; Lipid droplet-associated protein; PERI; Perilipin A; Perilipin; Perilipin-1; PerilipinA; PLIN; PLIN1; PLIN1_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human Perilipin-1 around the phosphorylation site of Ser522.

Uniprot:

O60240(PLIN1_HUMAN) >>Visit HPA database.

Gene(ID):
PLIN1(5346)
Expression:
O60240 PLIN1_HUMAN:

Detected in adipocytes from white adipose tissue (at protein level) (PubMed:27832861). Detected in visceral adipose tissue and mammary gland (PubMed:9521880).

Sequence:
MAVNKGLTLLDGDLPEQENVLQRVLQLPVVSGTCECFQKTYTSTKEAHPLVASVCNAYEKGVQSASSLAAWSMEPVVRRLSTQFTAANELACRGLDHLEEKIPALQYPPEKIASELKDTISTRLRSARNSISVPIASTSDKVLGAALAGCELAWGVARDTAEFAANTRAGRLASGGADLALGSIEKVVEYLLPPDKEESAPAPGHQQAQKSPKAKPSLLSRVGALTNTLSRYTVQTMARALEQGHTVAMWIPGVVPLSSLAQWGASVAMQAVSRRRSEVRVPWLHSLAAAQEEDHEDQTDTEGEDTEEEEELETEENKFSEVAALPGPRGLLGGVAHTLQKTLQTTISAVTWAPAAVLGMAGRVLHLTPAPAVSSTKGRAMSLSDALKGVTDNVVDTVVHYVPLPRLSLMEPESEFRDIDNPPAEVERREAERRASGAPSAGPEPAPRLAQPRRSLRSAQSPGAPPGPGLEDEVATPAAPRPGFPAVPREKPKRRVSDSFFRPSVMEPILGRTHYSQLRKKS

PTMs - O60240 As Substrate

Site PTM Type Enzyme
T8 Phosphorylation
T40 Phosphorylation
S53 Phosphorylation
Y58 Phosphorylation
S81 Phosphorylation P17612 (PRKACA)
T85 Phosphorylation
S126 Phosphorylation
S130 Phosphorylation
S174 Phosphorylation
S183 Phosphorylation
S211 Phosphorylation
S277 Phosphorylation
T299 Phosphorylation
T301 Phosphorylation
T306 Phosphorylation
T314 Phosphorylation
S374 Phosphorylation
S382 Phosphorylation
S384 Phosphorylation
Y401 Phosphorylation
S408 Phosphorylation
S436 Phosphorylation
S440 Phosphorylation
S461 Phosphorylation
T476 Phosphorylation
S497 Phosphorylation
S499 Phosphorylation
S504 Phosphorylation
Y515 Phosphorylation
S516 Phosphorylation
S522 Phosphorylation

Research Backgrounds

Function:

Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels.

PTMs:

Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet (By similarity).

Subcellular Location:

Endoplasmic reticulum. Lipid droplet.
Note: Lipid droplet surface-associated.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in adipocytes from white adipose tissue (at protein level). Detected in visceral adipose tissue and mammary gland.

Subunit Structure:

Interacts with ABHD5 (By similarity). Interacts with CIDEC. Interacts with AQP7.

Family&Domains:

Belongs to the perilipin family.

Research Fields

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

References

1). Hormone-sensitive lipase protects adipose triglyceride lipase-deficient mice from lethal lipotoxic cardiomyopathy. JOURNAL OF LIPID RESEARCH, 2022 (PubMed: 35283217) [IF=6.5]

Application: WB    Species: Mice    Sample: cardiac tissues

Fig. 2 Expression levels of members of the PLIN family in cardiac tissues. A: Western blot analysis of PLIN1, PLIN2, PLIN3, and PLIN5 in the cardiac ventricles of Wt, AKO, cHSL, and AKO+cHSL (A+H) mice: 20 μg of protein sample was subjected to SDS-PAGE, transferred to nitrocellulose membranes, and probed with an appropriate antibody. Four mice per group were analyzed, and the representative blots are presented. Note that PLIN1 was detected after an extremely long exposure. The graphs present the densitometric analysis of protein expression normalized to that of GAPDH. Values are presented as the mean ± SEM of four mice/group. B: mRNA expression levels of PLIN1, PLIN2, PLIN3, and PLIN5 in the cardiac ventricles of Wt, AKO, cHSL, and AKO+cHSL (A+H) mice. The expression levels were determined via RT-qPCR and normalized to GAPDH expression. Values are presented as the mean ± SEM of four mice per group. ∗P < 0.05, ∗∗P < 0.01 versus Wt mice; †P < 0.05, ††P < 0.01 versus AKO mice.
2). A label-free amperometric immunosensor with improved electrocatalytic 3D braided AuPtCu-SWCNTs@MoS2-rGO for human growth differentiation factor-15 detection. Analytical Methods, 2022 (PubMed: 35315459) [IF=3.1]

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